Female Fig (Ficus carica var. domestica) Studies on Genetic Diversity

Abstract

Turkey's female fig gene resources is valuable both economically and culturally. It is important to protect this wealth and transfer it for generations. However, as in other agricultural products; there is a genetic erosion risk in figs due to climate, human activities and environmental reasons. In order to ensure the sustainability of female fig genetic resources in the world countries as in Turkey, studies are carried out for the protection and enrichment of these resources. Collection, characterization, evaluation etc. are in these studies. The places where fig genetic resources are protected; botanical gardens, fig field gene banks, producer gardens or natural habitats. For example, fig field gene bank in Turkey, 354 fig genotypes are conserved. Studies on figs are very important in terms of creating a source for new studies, and subjects such as passport and characterization information are also recorded. In this article, studies and national approaches on the protection of female fig biodiversity in the world and in Turkey have been tried to be revealed and some recent studies on female fig genetic diversity have been summarized. Keywords: Selection, characterization, climate, molecular, breeding

Keywords

• Selection
• characterization
• climate
• molecular
• breeding

DİŞİ İNCİR (Ficus carica var. domestica) GENETİK ÇEŞİTLİLİĞİ ÜZERİNE YAPILAN ÇALIŞMALAR

Öz

Türkiye’nin incir genetik kaynakları, hem ekonomik hem de kültürel yönden değerlidir. Bu zenginliğin korunması ve kuşaklar boyu aktarılması önem taşımaktadır. Ancak, diğer tarımsal ürünlerde olduğu gibi; iklim, insan faaliyetleri ve çevresel nedenlerle incirde de genetik erozyon riski bulunmaktadır. Türkiye’de olduğu gibi Dünya ülkelerinde de dişi incir genetik kaynaklarının sürdürülebilirliğinin sağlanması için, bu kaynakların korunmasına, zenginleştirilmesine yönelik çalışmalar yapılmaktadır. Bu çalışmalar içinde; toplama, karakterizasyon, değerlendirilme gibi konular yer almaktadır. İncir genetik kaynaklarının korunduğu yerler; botanik bahçeleri, incir arazi gen bankaları, üretici bahçeleri ya da doğal habitat olabilir. Örneğin Türkiye incir arazi gen bankasında, 354 incir genotipi muhafaza altına alınmıştır. İncir üzerine yapılan çalışmalar yeni yapılacak olan çalışmalara da kaynak oluşturması açısından çok önemli olup, pasaport, karakterizasyon bilgileri gibi konular da kayıt altına alınmaktadır. Bu makalede, Dünya’da ve Türkiye’de dişi incir biyoçeşitliliğinin korunması üzerine, yapılan çalışmalar ile ülkesel yaklaşımlar ortaya konmaya çalışılmış ve dişi incir genetik çeşitliliği üzerine şimdiye kadar yapılan güncel bazı Ar-Ge çalışmaları özetlenmiştir.

Anahtar Kelimeler

• Seleksiyon
• Karakterizasyon
• iklim
• moleküler
• ıslah

Kaynakça

1. Abbasi, K. ve Arji, I. (2014). Pomological diversity of fig (Ficus carica L.) accessions of Kermanshah, Iran. J. Bio. & Env. Sci.(JBES). 5(3), 202-209. ISSN: 2220-6663. https://www.yumpu.com/en/document/read/59648091/pomological-diversity-of-fig-ficus-carica-l-accessions-of-kermanshah-iran.
2. Abdolinejad, R., Shekafandeh, A. ve Jowkar, A. (2021). In vitro tetraploidy induction creates enhancements in morphological, physiological and phytochemical characteristics in the fig tree (Ficus carica L.). Plant Physiology and Biochemistry. 166, 191-202. https://doi.org/10.1016/j.plaphy.2021.05.047.
3. Achtak, H., Oukabli, A., Ater, M., Satoni, S., Kjellberg, F. ve Khadari,. B. (2009). Microsatellite markers as reliable tools for fig cultivar identification. J. Amer. Soc. Hort. Sci. 134(6), 624–631. https://doi.org/10.21273/JASHS.134.6.624.
4. Achtak, H., Ater, M., Oukabli, A., Santoni, S., Kjellberg, F. ve Khadari, B. (2010). Traditional agroecosystems as conservatories and incubators of cultivated plant varietal diversity: the case of fig (Ficus carica L.) in Morocco. BMC Plant Biology. 10, 28. https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-28#auth-Sylvain-Santoni
5. Akbulut, M., Ercisli, S. ve Karlidag, H. (2009). RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey. Genet. Mol. Res. 8(3), 1109–1115. https://doi.org/ 10.4238/vol8-3gmr634.
6. Akin, M., Poljuha, D., Eyduran, SP., Ercisli, S. ve Radunic, M. (2021). SSR based molecular characterization of local fig (Ficus carica L.) germplasm in Northeastern Turkey. Erwerbs-Obstbau. 63, 387–392. https://doi.org/10.1007/s10341-021-00596-0.
7. Aksoy, U., Şahin, N., Ürel, N. ve Özkan, R. (1994). İncir araştırmaları projesi Sarılop klon seleksiyonu uygulama projesi. İncir Araştırma Enstitüsü, İncirliova-Aydın
8. Aksoy, U., Can, H.Z., Misirli, A., Kara, S., Seferoglu, H.G. ve Sahin, N. (7 Mayıs 2003). Fig (Ficus carica L.) selectıon study for fresh market in Western Turkey. II International Symposium on Fig.( ss. 197-203), Caceres, Spain. https://doi.org/10.17660/ActaHortic.2003.605.29

9. Alaklabi, A. (2021). Ficus species genetic diversity based on ınternal transcribed spacer (ITS) region analysis. Egyptian Academic Journal of Biological Sciences. 12(1), 21-27. https://doi.org/10.21608/eajbsh.2021.148134.
10. Alba, V., Montemurro, C., Sabetta, W., Pasqualone A. ve Blanco, A. (2009). SSR-based identification key of cultivars of Olea europaea L. diffused in Southern-Italy. Scientia Hort.123(1),11–16. https://doi.org/ 10.1016/j.scienta.2009.07.007.
11. Aljane, F., Ferchichi, A. ve Boukhris, M. (16 Mayıs 2008). Pomological characteristics of local fig (Ficus carica) cultivars in Southern Tunisia. III International Symposium on Fig. (ss. 123-128), Vilamouran, Portugal. https://doi.org/ 10.17660/ActaHortic.2008.798.15
12. Aljane, F. ve Essid, A. (2017). Characterization and assessment of fig (Ficus carica L.) genetic resources in Tunisia: an overview. Euro-Mediterranean Conference for Environmental Integration. Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions, Advances in Science, Technology & Innovation.1355-1356. https://doi.org/10.1007/978-3-319-70548-4_395.
13. Ammar, A., Aissa, I.B., Mars, M. ve Gouiaa, M. (2020 a.).Comparative physiological behavior of fig (Ficus carica L.) cultivars in response to water stress and recovery. Scientia Horticulturae. 260(27),108881. https://doi.org/10.1016/j.scienta.2019.108881.
14. Anwar, UH., Shazia, S. ve Alia, A. (2021). Species diversity and ethnobotanical inventory of wild flora used by the folk community of Shinghar. Nusantara Bioscience. 13(.2),148-157. https://doi.org/10.13057/nusbiosci/n130203.
15. Aradhya, MK., Stover, E., Velasco, D. ve Koehmstedt, A. (2010). Genetic structure and differentiation in cultivated fig (Ficus carica L.). Genetica. 138(6), 681–694. https://doi.org/10.1007/s10709-010-9442-3.
16. Ayar, A., Belge, A., Gülce, M., Ertan, B., Birol, D. ve Kargıcak, M. (2019). Tescile esas Sarılop klonlarının meyve kalite parametreleri yönünden incelenmesi. İncirde çeşit geliştirme projesi. Sonuç Raporu No: TAGEM/BBAD/16/A0B/P07/02. İncir Araştırma Enstitüsü Müdürlüğü, AYDIN.
17. Ayar, A. ve Seferoğlu, H.G. (2021). Investigations on yield and dried quality of Sarılop fig clones for further registration.In M Pakyürek (Ed.). Current Studies on Fruit Size. Chapter 2.(ss:39-72). IKSAD Publications, ISBN:978-625-8423-25-9.
18. Ayar, A., Şahin, B., Mutlu, D., Doğan, Ö. ve Özen, M. (2021). Studies on local Fethiye Kaya fig clones and other fig genotypes ın Fethiye and Seydikemer Districts of Muğla Province. In M Pakyürek (Ed.). Current Studies on Fruit Science. (ss:73-94). IKSAD Publications, ISBN:978-625-8423-25-9.
19. Ayar, A., (2022). İncir (Ficus carica L.) arazi gen bankaları ve çalışmaları. Biyolojik Çeşitlilik ve Koruma. 15(3),308-319. https://doi.org/10.46309/biodicon.2022.1144871.
20. Ayar, A., Şahin, B., Mutlu, D., Özen, M., Belge, A. ve Karacaoğlan, Ç. (2022). Fig (Ficus carica var. domestica L.) genetic resources conservation and characterization. Uluslararası Doğu Anadolu Fen ve Mühendislik Tasarım Dergisi. 5 (1), 1-19. https://doi.org/10.47898/ijeased.1197081.
21. Ayar, A., Özen, M., Şahin, B., Mutlu, D., Belge, A., Özkul, M., Özkul (Dağlı), M., Karataş, K. ve Aksu, Ü. (2015-2023). İncir (Ficus carica L.) genetik kaynakları ve muhafazası. Sürekli Proje. İncir Araştırma Enstitüsü. AYDIN.
22. Ayar, A. ve Karacaoğlan, Ç. (2023). İncir Sütü-Fisin-Lateks. Uluslararası Anadolu Ziraat Mühendisliği Bilimleri Dergisi. 5(1), 12-20. https://dergipark.org.tr/tr/pub/uazimder/issue/76026/1121294.
23. Badami, A. (2021). Managing the historical agricultural landscape in the Sicilian Anthropocene context. The landscape of the valley of the temples as a time capsule. Special Issue Historical Ecology and Landscape, Archaeology: Cross-Disciplinary Approaches to the Long Anthropocene. 13(8), 4480. https://doi.org/10.3390/su13084480
24. Balas, F. C., Osuna, M. D., Domínguez, G., Pérez-Gragera, F. ve López-Corrales, M. (2014). Ex situ conservation of underutilised fruit tree species: establishment of a core collection for Ficus carica L. using microsatellite markers (SSRs). Tree Genetics & Genomes. 10(3), 703–710. https://doi.org/10.1007/s11295-014-0715-3
25. Baraket, G., Chatti, K., Saddoud, O., Abdelkarim, A.B., Mars, M., Trifi, M. ve Hannachi, A.S. (2011). Comparative assessment of SSR and AFLP markers for evaluation of genetic diversity and conservation of fig, Ficus carica L., genetic resources in Tunisia. In O. Debbabi (Ed.), Plant Molecular Biology Reporter. 29, 171–184. https://doi.org/10.1007/s11105-010-0217-x
26. Basheer-Salimia, R., Awad, M., Hamdan, Y.ve. Shtaya, M. (2012). Genetic variability of some palestinian fig (Ficus carica L.) genotypes based on pomological and morphological descriptors. An - Najah Univ. J. Res. 27(2013),83-110. https://doi.org/10.35552/anujr.a.27.1.5
27. Bellon, MR. (2009). Do we need crop landcraces fort he future? Realazing the global option value of in situ conservation. In A. Konteleon U.Pascual and M. Smale (Ed.) Agrobiodeversity and Economic Development, (ss. 51-61) London and New York Routledge.
28. Bibliothèque Louvre (2017). Nina M.Davis, plate 3. https://www.lessingimages.com (Erişim: Haziran 2022).
29. Bona, A. ve Iordănescu, OA. (2019). Researches regarding fruit quality in some fig (Ficus carica L.) Genotypes, grown in Orșova, University of Agricultural Science and Veterinary Medicine, Mehedinți County. JOURNAL of Horticulture, Forestry and Biotechnology. 23(4), 18-22.
30. Bubola, K., Brkić Šetić, E., Krapac, M ve Novoselić, A. (31 Mayıs 2021). Preliminary characterization of morphological and sensorial profile of the fresh fig (Ficus carica L.) fruits obtained from four croatian cultivars. VI International Symposium on Fig, Rovinj, Croatia.
31. Cabrita, L.F., Aksoy, U., Hepaksoy, S. ve Leit~ao, J.M. (2001). Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Scientia Hort. 87(4), 261–273. https://doi.org/10.1016/S0304-4238(00)00181-3.
32. Chalak, L., Baydoun, S.A. ve Jaradat, A.A. (18 Eylül 2020). Genetic resources of fruit trees in the fertile crescent: a hotspot heritage. International Symposium on Survey of Uses of Plant Genetic Resources to the Benefit of Local Populations.(ss.77-84), Madagascar. https://doi.org/10.17660/ActaHortic.2020.1267.13.
33. Chatti, K., Saddoud, O., Salhi-Hannachi, A., Mars, M., Marrakchi, M. ve Trifi, M. (2007). Analysis of genetic diversity and relationships in a Tunisian Fig (Ficus carica) germplasm collection by random amplified microsatellite polymorphisms. Journal of Integrative Plant Biology. https://doi.org/10.1111/j.1744-7909.2007.00396.x.
34. Chatti, K., Baraket, G., Abdelkrim, A.B., Saddoud, O., Mars, M., Trifi, M. ve Hannachi, AM. (2010). Development of molecular tools for characterization and genetic diversity analysis in Tunisian Fig (Ficus carica) cultivars. Biochemical Genetics. 48(9-10), 789–806. https://doi.org/ 10.1007/s10528-010-9360-1
35. Chessa, I. ve Nieddu, G. (2003). Evaluation of fruit tree genetic resources in a Mediterranean Secondary Center of biodiversity. XXVI Int. Hort. Congress. Acta. Hort. 623,239-247.https://doi.org/ 10.17660/ActaHortic.2003.623.26
36. Chessa, I. ve Nieddu, G. (2005). Analysis of diversity in the fruit tree genetic resources from a Mediterranean Island. Genetic Resources and Crop Evolution. 52(3), 267–276. https://doi.org/10.1007/s10722-005-7906-6
37. Ciarmiello, L.F., Piccirillo P., Carillo P., Luca, A. ve Woodrow, P. (2015). Determination of the genetic relatedness of fig (Ficus carica L.) accessions using RAPD fingerprint and their agro-morphological characterization. South African Journal of Botany. 97, 40-47. https://doi.org/10.1016/j.sajb.2014.11.012
38. Crisosto, C. H., Bremer, V., Ferguson, L. ve Crisosto, M.G. (2010). Evaluating quality attributes of four fresh fig (Ficus carica L.) cultivars harvested at two maturity stages. American Society for Horticultural Science 45(4), 707-710. https://doi.org/10.21273/HORTSCI.45.4.707
39. Çalışkan, O. (2012). Türkiye'de sofralık incir yetiştiriciliğinin mevcut durumu ve geleceği. Uludağ Üniversitesi Ziraat Fakültesi Dergisi , 26 (2) , 71-87 . https://dergipark.org.tr/tr/pub/ziraatuludag/issue/16760/174262.
40. Çalişkan, O. ve Polat, A. (2011). Phytochemical and antioxidant properties of selected fig (Ficus carica L.) accessions from the eastern Mediterranean region of Turkey. Sci. Hort. 128(4), 473-478. https://doi.org/10.1016/j.scienta.2011.02.023.
41. Caliskan, O., Polat, A.A., Celikkol, P. ve Bakir, M. (2012b). Molecular characterization of autochtho-nous Turkish fig accessions. J. Agr. Res.10(1), 130–140. https://doi.org/10.5424/sjar/2012101-094-11.
42. Dalkilic, Z., Mestav, H.O. ve Dalkilic, G.G. (2011). Genetic diversity of male fig (Ficus carica caprificus L.) genotypes with random amplified polymorphic DNA (RAPD) markers. Afr. J. Biot. 10 (4), 519–526. https://aperta.ulakbim.gov.tr/record/21105.
43. Darjazi, B.B. (2011). Morphological and pomological characteristics of fig (Ficus carica L.) cultivars from Varamin, Iran. African Journal of Biotechnology. 10(82), 19096-19105. https://doi.org/10.5897/AJB.9000367
44. Dean, R., Dahlberg, J., Hopkins, M., Mitchell, S. ve Kresovich, S. (1999). Genetic redundancy and diversity among ‘orange’ accessions in the us national sorghum collection as assessed with simple sequence repeat (SSR) markers. Crop Sci. 39(4),1215–1221. https://doi.org/10.2135/cropsci1999.0011183X003900040043x
45. Debbabi, O.S., Mnasri, S., Abedelaali, S.B. ve Mars, M. (2016). Fruit genetic resources facing ıncreasing climate uncertainty. Chapter. Book Applied Mathematics and Omics to Assess Crop Genetic Resources for Climate Change Adaptive Traits. p:7 eBook ISBN 9781315369006.
46. Debbabi, O.S., Khanfir, E., Mohamed, A.D. ve Massaoud, M.M. (2021). Ethnobotanical and on farm genetic surveys of Fig (Ficus carica L.) genetic resources in Kerkennah Islands. https://doi.org/10.22059/ijhst.2020.310191.396
47. Delgado-Paredes, G E., Díaz, CV., Ibañez, BE., Sernaqué, PB. ve Idrogo, CR. (2021). In vitro tissue culture in plants propagation and germplasm conservation of economically important species in Peru. Sci. Agropecuaria. 12 (3). http://dx.doi.org/10.17268/sci.agropecu.2021.037.
48. Djuric, G., Ilic, P., Stanivukovic, S., Micic, N., Vego, D., Saravanja, P. ve Ivankovic, A. (23-26 October 2014). Preliminary pomological and biochemical characterization of fig (Ficus carica L) germplasm collected in Herzegovina. Fifth International Scientific Agricultural Symposium Agrosym. (ss.257-267). Bosnia and Herzegovina.
49. Doğan, A., Kurt, H. ve Özrenk, K. (2021). İncir (Ficus carica L.) genotipleri arasındaki fenotipik varyasyonun kümeleme ve temel bileşen analizi metodu ile belirlenmesi. Turk J Agric Res. 8(3), 282-294. https://doi.org/10.19159/tutad.978186
50. Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. ve Adıgüzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı. Ankara, Türkiye: Türkiye Tabiatını Koruma Derneği ve Yüzüncü Yıl Üniversitesi.
51. El Oualkadi, A. ve Hajjaj, B. (2019a). Assessment of the local varieties of the fig (Ficus carica L) tree in Moukrisset area of Morocco. Agriculture and Biotechnology. 4-6, 1642-1647. https://doi.org/10.22161/ijeab.46.1
52. El Oualkadi, A. ve Hajjaj, B. (2019b). Characterization of some local varieties of fig tree (Ficus carica L.) in Oued Laou region of Morocco. International Journal of Environment, Agriculture and Biotechnology. 4-5, 1554-1558. https://doi.org/ 10.22161/ijeab.45.41
53. Elwakil, H., Fathallah Zaitoun, A., Weheda, B., Mohamed Abushady, A., Elsaid Khalid, A. ve Abo Elmaaty, A.E., (2021). Genetical and morphological studies on Ficus trees. Journal of the Advances in Agricultural Researches. 26 (2), 60-73. https://doi.org/10.21608/jalexu.2021.171573.
54. Ercisli, S. (2004). A short review of the fruit germplasm resources of Turkey, Genetic Resources and Crop Evolution. 51(4), 419–435. http://dx.doi.org/10.1023/B:GRES.0000023458.60138.79.
55. Ergül, A., Büyük, BP., Hazrati, N., Yılmaz, F., Kazan, K., Arslan, N., Özmen, CY., Aydın, SS., Bakır, M., Tan, N., Kösoğlu, İ. ve Çobanoğlu, F. (2021). Genetic characterisation and population structure analysis of Anatolian figs (Ficus carica L.) by SSR markers. Folia Horticulturae. 33(1), 49–78. https://doi.org/10.2478/fhort-2021-0005.
56. Eroğlu, A. Ş. (1982). İncir seleksiyonu incir araştırmaları projesi. Erbeyli Zirai Araştırma Enstitüsü Müdürlüğü, Aydın.
57. Esquinas-Alc_azar, J. (2005). Protecting crop genetic diversity for food security: Political, ethical and technical challenges. Nat. Rev. Genet. 6, 946– 953. http://dx.doi.org/10.1038/nrg1729.
58. Gaaliche, B., Saddoud, O. ve Mars, M. (2012). Morphological and pomological diversity of fig (Ficus carica L.) cultivars in Northwest of Tunisia. Agronomy. 2012(326461), 9. https://doi.org/ 10.5402/2012/326461
59. Gautam, I.P. ve Gotame, T.P. (2020). Diversity of native and exotic fruit genetic resources in Nepal. Journal of Nepal Agricultural Research Counci., 6(1), 44-55. https://doi.org/10.3126/jnarc.v6i0.28114
60. Gebauer, J., Luedeling, E., Hammer,K., Nagieb, M. ve Buerkert, A. (2007). Mountain oases in northern Oman: An environment for evolution and in situ conservation of plant genetic resources. Genetic Resources and Crop Evolution. 54, 465-481. https://doi.org/10.1007/s10722-006-9205-2
61. GIZ. (2013). Mise en place d’un systeme de suivi evaluation de I’adaptation au changement climatique cas del’agriculture.
62. Giraldo, E., Viruel, M.A., Corrales, ML. ve Hormaza, J.I. (2005). Characterisation and cross-species transferability of microsatellites in the common fig (Ficus carica L.). J. The Journal of Hort. Sci. Biotechnol. 80(2), 217–224. https://doi.org/ 10.1080/14620316.2005.11511920
63. Giraldo, E., Corrales, ML. ve Hormaza, JI. (2008). Optimization of the management of an ex-situ germplasm bank in common fig with SSRs. Journal of the American Society for Horticultural Science. 133(1), 69-77. https://doi.org/ 10.21273/JASHS.133.1.69
64. Giraldo, E., Corrales, M.L. ve Hormaza, J.I. (2010). Selection of the most discriminating morphological qualitative variables for characterization of fig germplasm. J. Amer. Soc. Hort. Sci. 135(3), 240–249.
65. Gözlekçi, Ş. (2011). Pomologıcal traits of fıg (Ficus carica l.) Genotypes collected in the West Mediterranean Region in Turkey. The Journal of Animal and Plant Sciences. 21(4),646-652. https://aperta.ulakbim.gov.tr/record/20323
66. Haffar, S., Baraket, G., Usai, G., Aounallah, A., Mustapha,SB., Abdelkrim, AA. ve Hannachi, AS. (2022). Conserved DNA-derived polymorphism as a useful molecular marker to explore genetic diversity and relationships of wild and cultivated Tunisian figs (Ficus carica L.). Trees. 36, 1-13. https://doi.org/10.1007/s00468-021-02244-2
67. Halewood, M., Lopez Noriega, I. ve Louafi, S. (2013a). The global crop commons and access and benefit-sharing laws: Examining the limits of ınternational policy support for the collective pooling and management of plant genetic resources. Crop genetic resources as a global commons: Challenges in international law and governance. In M Halewood, I. Lopez Noriega and S. Louafi (Eds.). Earthscan Publications, 1-36. ISBN:978-1-84407-893-6,
68. Halewood, M., Sood, R., Hamilton, R. S., Amri, A., den Houwe, I. V, Roux, N., Dumet, D., Hanson, J., Upadhyaya, H. D., Jorge, A. ve Tay, D. (2013b). Changing rates of acquisition of plant genetic resources by International genebanks: setting the scene to monitor an ımpact of the international treaty. In Crop Genetic Resources as a Global Commons, ISBN:9781849776813, Routledge.
69. Hazarhun, G. ve Kumral, N.A. (2021). Hasat öncesi incir meyvelerinde ethephone kullanımının oluşturduğu kalıntı riskinin belirlenmesi, Bursa Uludag Üniv. Ziraat Fak. Derg. 35(2), 299-312. http://www.uludag.edu.tr/ziraatdergi.
70. Hernandez, P. (2005). Comparison among available marker systems for cereal introgression breeding: A practical perspective. Euphytica. 146(1-2), 95–100. https://doi.org/10.1007/s10681-005-7676-4
71. Hoshino, A.A., Bravo, J.P., Nobile, P.M. ve Morelli, K.A. (2012). Microsatellites as tools for genetic diversity analysis, genetic diversity in microorganisms. https://doi.org/10.5772/35363
72. Hssaini, L., Charafi J., Hanina, H., Ennahli, S., Mekaoui, A., Mamouni, A. ve Razouk, R. (2019a). Comparative analysis and physio-biochemical screening of an ex-situ fig (Ficus carica L.) collection. Horticulture, Environment, and Biotechnology. 60(5), 671–683. https://doi.org/10.1007/s13580-019-00170-4
73. Hssaini, L., Hanine, H., Razouk, R., Ennahli, S., Mekaoui, A. ve Guirrou, I. (2019b). Diversity Screening of Fig (Ficus carica L.) Germplasm through Integration of Morpho-agronomic and Biochemical Traits. 939-958. https://doi.org/10.1080/15538362.2019.1700871
74. Hssaini, L., Hanine, H., Razouk, R., Ennahli, S., Mekaoui, A., Ejjilani, A. ve Charafi, J. (2019c). Assessment of genetic diversity in Moroccan fig (Ficus carica L.) collection by combining morphological and physicochemical descriptors. Genetic Resources and Crop Evolution. 67(2), 457–474. https://doi.org/10.1007/s10722-019-00838-x
75. Hssaini, L., Charafi, J., Razouk, R., Hernández, F., Fauconnier, M.L. ve Ennahli, S. (2020). Assessment of morphological traits and fruit metabolites in eleven fig varieties (Ficus carica L.), 8, 28. https://doi.org/10.1080/15538362.2019.1701615.
76. Ikegami, H., Nogata, H., Hirashima, K.ve Awamura, M. (2009). Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers. Genet. Resources Crop Evol. 56(2), 201– 209. https://doi.org/10.1007/s10722-008-9355-5
77. Karacaoğlan, Ç. ve Can, H. Z. (2023). Farklı potasyum dozları ile gübrelemenin incirde meyve kalitesine etkisi (Ficus carica L. Cv Sarılop). Uluslararası Anadolu Ziraat Mühendisliği Bilimleri Dergisi, 5(1), 1-11. https://dergipark.org.tr/tr/pub/uazimder/issue/76026/1170061.
78. Khadari, B., Hochu, I., Santoni, S. ve Kjellberg, F. (2001). Identification and characterization of microsatellite loci in the common fig (Fcus carica L.) and representative species of the genus Ficus. Mol. Eco. Res. 1, 191–193. https://doi.org/10.1046/J.1471-8278.2001.00072.X
79. Khadari, B., Oukabli, A., Ater, M., Mamouni, A., Roger, J.P. ve Kjellberg, F. (2004). Molecular characterization of Moroccan fig germplasm using intersimple sequence repeat and simple sequence repeat markers to establish a reference collection. Hort. Science. 40(1), 29–32. https://doi.org/10.21273/HORTSCI.40.1.29
80. Khadari, B., Harci, C., Santoni, S.ve Kjellberg, F. (2005). Contrasted genetic diversity and differentiation among Mediterranean populations of Ficus carica L. A study using mtDNA RFLP. Genetic Resources and Crop Evolution. 52(1), 97-109. https://doi.org/10.1007/s10722-005-0290-4
81. Khadari, B., Roger, J.P., Ater, M., Achtak, H., Oukabli, A. ve Kjellberg, F. (16 Mayıs 2008). Moroccan Fig presents specific genetic resources: A high potential of local selection. III International Symposium on Fig. (ss. 33-37). Portugal.
82. Khadari, B. (22 August 2010). Ex situ management of fig (Ficus carica L.) Genetic resources: towards the establishment of the Mediterranean reference collectıon. XXVIII International Horticultural Congress on Science and Horticulture for People Fruits and Nuts.(ss 67-74). Lisbon.
83. Khadivi , A., Anjam, R. ve Anjam, K. (2018). Morphological and pomological characterization of edible fig (Ficus carica L.) to select the superior trees. Scientia Horticulturae. 238, 66-74. https://doi.org/10.1016/j.scienta.2018.04.031
84. Knap, T., Baruca Arbeiter, A., Jakše, J., Čizmović, M., Adakalić, M., Popović, R., Lazović, B., Strikić, F., Podgornik, M. ve Bandelj, D. (31 Ağustos 2017). Diversity of figs (Ficus carica L.) from the east Adriatic coast. V International Symposium on Fig. (ss 11-16), Italy.
85. Kokaj, T.K. (28 Nisan 2003). Studies on local fig (Ficus carica L.) germplasm in Albania. II International Symposium on Fig. ActaHort.605. Spain. https://doi.org/ 10.17660/ActaHortic.2003.605.11
86. Kokaj, T.K. (2018). Genotype identifıcation of Roshnik Variety. International Journal of Agriculture, Environment and Bioresearch. 3(06).
87. Kokaj, T. (2019). Clonal selection in Cipull cv, Kraps I Zı cv, Melacak cv at Tirana Region in Albania. International Journal of Agriculture, Environment and Bioresearch. 4(02),41-46. ISSN:2456-8643.
88. Kokaj, T. (2021). Investigation and value of qualitative and quantitative characteristics 10 of genotypes of figs gene bank, Institute of Plant Genetic Resource, Albania, Tirane. International Journal of Food Science and Agriculture. 5(3), 448-454. http://dx.doi.org/10.26855/ijfsa.2021.09.015
89. Koyuncu, M. A. (2004). Promising fig (Ficus carica L.) genetic resources from Birecik (Urfa) Region of Turkey Süleyman Demirel University, Faculty of Agriculture, Department of Horticulture, Isparta, Turkey. Europ.J.Hort.Sci. 69(4), 153–158. ISSN 1611-4426.
90. Krishna, J.B. ve Adhikari, Rc. (21-22 June 2018). Economical plant and crop species behind the eyes of researchers and policy makers. Conference: Working Groups of Agricultural Plant Genetic Resources (APGRs) in Nepal BK Joshi and R Shrestha,(Ed.). Proc. National Workshop,( s.26), Kathmandu. NAGRC, Nepal
91. Kutlu, E. ve Aksoy, U. (1998). Further evaluation of selected Sarilop (=Calimyrna) clones. Ege University Faculty of Agriculture. Department of Horticulture. 265-269.
92. Küden, A.B. ve Tanriver, E. (1 December 1998). Plant genetic resources and selection studies on figs in The East Mediterranean and South East Anatolia Regions I International Symposium on Fig. İzmir, Turkey. https://doi.org/10.17660/ActaHortic.1998.480.6.
93. Labaioui, A. ve Bouchoufi, K. (2021). Assessing the impact of climate change on land suitability for crops in El Hajeb province Morocco. AFRIMED Agricultural Journal AJ-Al Awamia. (132),65-90.
94. Limera, C., Sabbadine, S., Sweet, J.B. ve Mezzetti, B. (2017). New biotechnological tools for the genetic improvement of major woody fruit species. Frontiers in Plant Sciences. 15(8), 1418. https://doi.org/10.3389/fpls.2017.01418.
95. Mafrica, R., De Bruno, A., Piscopo, A., Poiana, M., Bruno, M. ve Caruso, T. (2 Eylül 2021). Cultivar and accessions of fig (Ficus carica L.) for breba production selected within the autochthonous germplasm of Calabria. South Italy.VI International Symposium on Fig. (ss. 29-34), Rovinj. https://doi.org/10.17660/ActaHortic.2021.1310.5.
96. Mars, M., Chebli, T. ve Marrakchi, M. (1998). Multivariate analysis of fıg (Ficus carica L.) germplasm in Southern Tunisia. I International Symposium on Fig. https://doi.org/10.17660/ActaHortic.1998.480.10.
97. Mir, MM., Kumar, A.,Umar, I., Mir, S.A., Rehman, M.U., Banday, S.A., Rather, G.H. ve Sibat, F. (2018). Characterization of fig (Ficus carica L.) germplasm in Central Kashmir of North Western Himalayan Region. Indian Journal of Plant Genetic Resources. 3(1), 57- 63, https://doi.org/10.5958/0976-1926.2018.00009.8
98. Morton, J.F. (2007). The impact of climate change on smallholder and subsistence agriculture. Proceedings of the National Academy of Sciences.104, 19680-19685. https://doi.org/ 10.1073/pnas.0701855104
99. Mkedder, I., Chahbar, M., Ilias, F. ve Gaouar, SBS. (2021). Characterization and morphological typology of fig variety (Ficus carica) in the Tlemcen Region. Genetics and Biodiversity Journal (GABJ). 6(1). https://doi.org/10.46325/gabj.v5i2.60
100. Nader, R.A., Rehab, M.A., Hayssam, MA., Mohamed, Z.M.S., Kamal, FA. ve Mohamed, SE. (2019). Morphological, pomological, and specific molecular marker resources for genetic diversity analyses in fig (Ficus carica l.) Hort. Sci. 54(8):1299–1309. https://doi.org/10.21273/HORTSCI14091-19
101. Nobre, J. ve Romano, A.( 24 June 1998). In vitro cloning of Ficus carica l. adult trees. I International Symposium on Fig. (ss.161-164). İzmir, Turkey. https://doi.org/10.17660/ActaHortic.1998.480.27
102. O’Donnell, K. ve Sharrock, S. (2017-2018). The contribution of botanic gardens to ex situ conservation through seed banking. Plant Diversity. 39(6). https://doi.org/10.1016/j.pld.2017.11.005
103. Oukabli, A., Mekaoui, A., Ibnouali-El-Aloui, M. ve Bari, A. (2008). Contribution to identifıcation of fıg (Ficus carica) genotypes tolerant to drought. III International Symposium on Fig. https://doi.org/10.17660/ActaHortic.2008.798.10.
104. Papadopoulou, K., Ehaliotis, C., Tourna, M., Kastanis, P., Karydis I. ve Zervakis, G. (2002). Genetic relatedness among dioecious Ficus carica L. cultivars by random amplified poly- morphic DNA analysis, and evaluation of agronomic and morphological characters. Genetica. 114(2),183–194. https://doi.org/10.1023/a:1015126319534
105. Perez-Jiménez, M., López, B., Dorado, G., Pujadas-Salvá A., Guzmán, G. ve Hernandez, P. (2012). Analysis of genetic diversity of southern Spain fig tree (Ficus carica L.) and reference materials as a tool for breeding and conservation, Hereditas. 149(3), 108-113. https://doi.org/10.1111/j.1601-5223.2012.02154.x
106. Podgornik, M., Vuk, I., Vrhovnik, I. ve Mavsar, D.B. (2010). A survey and morphological evaluation of fig (Ficus carica L.) genetic resources from Slovenia. Scientia Horticulturae. 125, 380-389. https://doi.org/10.1016/J.SCIENTA.2010.04.030
107. Polat, A.A. ve Çalışkan, O. (2010). Fruit characteristics of table fig (Ficus carica) cultivars in subtropical climate conditions of the Mediterranean region. New. Zealend Journal Crop. And Hor. Sci. 36(2), 107-115. https://doi.org/ 10.1080/01140670809510226
108. Primack, R.B. ve Rodrigues, E. (2006). Biologia da Conservação Planta, Portugu^es. https://www.academia.edu/40644193/Biologia_da_Conserva%C3%A7%C3%A3o_Primack_and_Rodrigues.
109. Primack, RB., Ellwood, E R., Gallinat, A. S. ve Miller-Rushing, A. J. (2021). The growing and vital role of botanical gardens in climate change research. New Phytologist. 231(3), 917-932. https://doi.org/10.1111/nph.17410.
110. Ramirez-Villegas, J., Challinor, A.J., Thornton, P.K.ve Jarvis, A. (2013). Implications of regional improvement in global climate models for agricultural impact research. Environmental Research Letters. 8: 024018. https://doi.org/10.1088/1748-9326/8/2/024018
111. Rattanpal H.S., Sidhu, G.S. ve Bons, H.K. (31 Ağustos 2017). Tree and fruit characteristics of fig cultivars grown in Punjab. ISHS Acta Horticulturae 1173: V International Symposium on Fig.(ss.117-120). Italy. https://doi.org/10.17660/ActaHortic.2017.1173.20
112. Rodrıgues, M.G.F., Momterio, L.N.H., Ferreira, A.F.A., Santos, T.P.D., Pavan, B.E., Neves, V.A.B. ve Boliani, A.C. (2018). Genetic variability in morphological characters among Fig tree accessions Fig genetic conservation. Genet. Mol. Res. 17(4). http://dx.doi.org/10.4238/gmr16039931
113. Sadder, MT., Alshomali, I., Ateyyeh, A. ve Musallam, A. (2021). Physiological and molecular responses for long term salinity stress in common fig (Ficus carica L.). Physiology and Molecular Biology of Plants. 27, 107–117. https://doi.org/10.1007/s12298-020-00921-z
114. Saddoud, O., Salhi-Hannachi, A., Chatti, K., Mars, M., Rhouma, A., Marrakchi, M. ve Trifi, M. (2005). Tunisian fig (Ficus carica L.) genetic diversity and cultivar characterization using microsatellite markers. Fruits. 60(2), 143-153. http://dx.doi.org/10.1051/fruits:2005018.
115. Saddoud, O., Chatti, K., Salhi-Hannachi, A., Mars, M., Rhouma, A., Marrakchi, M. ve Trifi, M. (2007). Genetic diversity of Tunisian figs (F. carica L.) as revealed by nuclear microsatellites. Hereditas. 144(4),149-157. https://doi.org/10.1111/j.2007.0018-0661.01967.x.
116. Salerno, G., Stinca, A., Giaccone, M., Scognamiglio, P. ve Basile, B. (31 Ağustos 2017). Ethnobotanical use of fig (Ficus carica L.) in southern Italy. ISHS Acta Horticulturae 1173: V International Symposium on Fig. (ss. 371-376). Italy. https://doi.org/10.17660/ActaHortic.2017.1173.64.
117. Sanchez, MA.J., Melgarejo, P., Hernandez, FCA. ve Martinez-Nicolas, J.J. (2003). Chemical and morphological characterization of four fig tree cultivars (Ficus carica L.) grown under similar culture conditions Acta Horticulturae, 605(605),33-36. https://doi.org/10.17660/ActaHortic.2003.605.3
118. Sánchez, RP., Remedios, MM.C. ve Sánchez Ángeles, M. (2016). Agro-morphological diversity of traditional fig cultivars grown in central-western Spain. Genetika. 48, 533-546. https://doi.org/10.2298/GENSR1602533P.
119. Sezen, I., Ercişli, S. ve Gözlekçi, Ş. (2014). Biodiversity of figs (Ficus carica l.) in Çoruh Valley of Turkey. Erwerbs-Obstbau. 56(4), 139–146. https://doi.org/10.1007/s10341-014-0222-6.
120. Simioniuc, D., Uptmoor, R., Friedt, W., Ordon F. ve Swiecicki, W. (2002). Genetic diversity and relationships among pea cultivars revealed by RAPDs and AFLPs. P. Breed. 121, 429–435. https://doi.org/10.1046/j.1439-0523.2002.733320.x
121. Simsek, M. ve Yildirim, H. (2010). Fruit characteristics of the selected fig genotypes. Afr. J. Biot. 9(37), 6056–6060. eISSN: 1684-5315.
122. Simsek, M., Kocatas, H. ve Cobanoglu, F. (2010). Table fıg (Ficus carica L.) selectıon in Midyat District of Mardin Province Selçuk Tarım ve Gıda Bilimleri Dergisi. 24 (3), 75-78.
123. Sımsek, M., Gulsoy, E., Kırar, M Z., Turgut, Y. ve Yucel, B. (2017). Identification and selection of some female fig genotypes from Mardin Province of Turkey. Pak. J. Bot. 49(2), 541-546.
124. Soliman, A., Hamdy, R. ve Mahdy, R. (2021). Numerical taxonomy of genus Ficus l. 1753 (Moraceae), wıth addition new record species to Egypt. https://doi.org/10.26842/binhm.7.2021.16.4.0429
125. Stănică, F., Butcaru, A.C., Asănică, A.C. ve Mihai, C.A. (2 Eylül 2021). Selection of several fig genotypes (Ficus carica L.) from the Romanian fig populations assessment. ISHS Acta Horticulturae 1310: VI International Symposium on Fig. (ss.15-20). Rovinj. https://doi.org/10.17660/ActaHortic.2021.1310.3.
126. Stewart, Jr. CN (Edit.). (2008). Plant biotechnology and genetics: principles, techniques, and applications. A John Wiley & Sons, Inc. 374 s. ISBN: 978-1-118-82012-4.
127. Stover, E. ve Aradhya, M. (16 Mayıs 2005-2008). Fig genetic resources and research at the US National Clonal Germplasm repository in Davis, California, p. 57–68. III Inter. Symp. on Fig, (ss.57-68), Portugal. https://doi.org/10.17660/ActaHortic.2008.798.6
128. Sugiura, T., Kuroda, H. ve Sugiura, H. (2007). Influence of the current state of global warming on fruit tree growth in Japan. Hort. Res. 6, 257–263. https://doi.org/10.2503/HRJ.6.257
129. Tan, A. (2010). Türkiye bitki genetik kaynakları ve muhafazası, Anadolu, J of AARI. 20(1), 9-37. https://dergipark.org.tr/tr/pub/anadolu/issue/1760/21732
130. Taylan, H. (2017). Herbal temalı Batı Anadolu sikkeleri [Yüksek Lisans Tez,. Adnan Menderes Üniversitesi Sosyal Bilimler Enstitüsü Arkeoloji Bölümü]
131. Thanopoulos, R., Chatzigeorgiou, T., Argyropoulou, K., Kostouros, NM. ve Bebeli, PJ. (2021). State of Crop Landraces in Arcadia (Greece) and in-situ Conservation Potential Institute of Plant Breeding and Genetic Resources. Diversity. 13(11), 558. https://doi.org/10.3390/d13110558
132. Trad, M., Gaaliche, B., Renard, C.M.G.C. ve Mars, M. (12 December 2012 a). Biodiversity and genetic resources of fig (Ficus carica L.) change from coastal to continental areas of Tunisia. Conference:.1st Congress of ATPBE. At: Douz, Tunisia.. https://doi.org/10.13140/2.1.1316.1609
133. Trad, M., Gaaliche, B., Renard, C.M.G.C. ve Mars, M. (2012b) Quality performance of 'smyrna' type figs grown under Mediterranean Conditions of Tunisia. Journal of Ormamental and Hort. Plants. 2(3),139-146. ISSN: 2251-6441
134. Trad, M., Gaaliche, B., Renard, C.M.G.C. ve Mars, M. (2013). Plant natural resources and fruit characteristics of fig (Ficus carica L.) change from coastal to continental areas of Tunisia. Journal of Agricultural Research and Development. 3(2), 022-025. ISSN: 2276-9897.
135. Tripathi, P. (2017). Principles, Strategies and practices of exploration, collection, characterization, evaluation and cataloging of plant genetic resources important fruit crops. https://doi.org/10.13140/RG.2.2.15781.60648.
136. UNESCO Birleşmiş Milletler Eğitim, Bilim ve Kültür Örgütü Türkiye Millî Komisyonu, (2022). Sürdürülebilir Kalkınma 2030 Hedefleri İhtisas Komitesi. https://www.unesco.org.tr/Pages/108/219/S%C3%BCrd%C3%BCr%C3%BClebilir-Kalk%C4%B1nma-2030-Hedefleri-%C4%B0htisas-Komitesi (Erşim: Haziran 2022).
137. Uzun, S. ve Yarılgaç, T. (2021). Germencik (Aydın) İlçesi’nde yetiştirilen Sarılop incirlerinde (Ficus carica L.) klon seleksiyonu, International Journal of Agricultural and Wildlife Sciences. 7(1), 1-8. https://doi.org/10.24180/ijaws.747500.
138. Velicevici, G., Madosa, E., Malaescu, M., Cretescu, I. ve Gorinoiu, G. (29 June-5 July 2017). Studies on genetic variability of the species Ficus carica L. in Western Romania. Section Advances in Biotechnology Conference: 17th International Multidisciplinary Scientific GeoConference (ss.977-982). Bulgaria.
139. Vermeulen, S.J., Challinor, A.J., Thornton, P.K., Campbell, B.M., Eriyagama, N., Vervoort, J.M., Kinyangi, J., Jarvis, A., Läderach, P., Ramirez-Villegas, J., Nicklin, K.J., Hawkins, E. ve Smith, D.R. (2013). Addressing uncertainty in adaptation planning for agriculture. Proc. Natl Acad. Sci.(PNAS) 110(21), 8357–8362. https://doi.org/10.1073/pnas.1219441110.
140. Vivaldi, G.A., Quartararo, A., Camposeo, S., Belloni, P., Volo, P. ve Caruso, T. (31 August 2017). Genetic diversity of early ripening breba accessions (Ficus carica L.) found in the “Pomona” Apulian collection. ISHS Acta Horticulturae 1173: V International Symposium on Fig. (ss.121-126). Italy. https://doi.org/10.17660/ActaHortic.2017.1173.21
141. Volis, S. (2016). How to conserve threatened Chinese plant species with extremely small populations? P. Diversity. 38,45–52. https://doi.org/10.1016/j.pld.2016.05.003.
142. Wang, L., Wang, C., Tian, Y. ve Jia, Y. (2007). Genetic diversity analysis of figs (Ficus carica L.) in shandong province by RAPD Technique. Department of Horticulture, Laiyang Agricultural College.
143. Zammouri, J. (1999). Inventaire des espèces et des varietes fruitières de la Vallée de Zammour. IRA Médenine, 13, Google scholar.
144. Zavodna, M., Arens, P., Van Dijk, P.J. ve Vosman, B. (2005). Development and characterization of microsatellite markers for two dioecious Ficus species. Mol. Ecol. Not. 5(2), 355–357. https://doi.org/10.1111/j.1471-8286.2005.00924.x
145. Zhou, H., Liao, J., Xia, Y.-P. ve Teng, Y.-W.(2013). Morphological characteristics for classifying evergreen azalea (Ericaceae) cultivars in China using numerical taxonomy. Pak. J. Bot. 45(2),593–598.