Çinko Oksit Nanoparçacıklarına Maruz Bırakılan Gammarus pulex’te Metallothionein Üzerine Etkisi

Öz

Nanopartikül (NP) ürünlerinin endüstride kullanımının artmasıyla birlikte ekosistem ve tüm organizmalar doğrudan ve dolaylı olarak NP’lerin etkisiyle risk altında olmaktadırlar. Bu çalışmada, tatlısularda yaşayan Gammarus pulex’in çinko (Zn) ve çinko oksit (ZnO) NP’lere maruz bırakılmasıyla metallotionein (MT) biyobelirteç yanıtları tespit edilmiştir. Denemede, kontrol grubu dahil toplam dört deneysel uygulama grubu (Kontrol, 10 ppm, 20 ppm, 40 ppm) oluşturulmuş ve üç tekerrür ile çalışılmıştır. Zn-NP’ne maruz bırakılan, 24. ve 96. saatlerde alınan Zn-NP uygulama örneklerinin MT seviyelerinde kontrole kıyasla artışa (p<0.05) neden olduğu belirlenmiştir. Benzer şekilde, ZnO-NP uygulama örneklerinin MT seviyelerinde de 96 saat örneklerinde kontrole kıyasla artışlara neden olduğu (p<0.05) belirlenmiş, ancak 24. saat uygulama örneklerinde MT seviyelerinde istatistiksel açıdan far bulunamamıştır (p<0,05). Çalışmada yapılan analizler sonucunda, Zn ve ZnO NP’lerin organizmada MT seviyelerinde artışa sebebiyet verdiği, bu artışın oksidatif stres yapabileceği öngörülmektedir. Nano boyuttaki malzemelerin artan kullanım alanları ile birlikte, çevre için büyük bir problem olarak karşımıza çıkabileceği belirlenmiştir.

Anahtar Kelimeler

• Metallotionein
• Gammarus pulex
• çinko nanopartikül
• çinkooksit nanopartikül

Effect on Metallothionein in Gammarus pulex Exposed to Zinc Oxide Nanoparticles

Öz

With the increase in the use of nanoparticle (NP) (<100nm) products in the industry, ecosystems, aquatic environments, all organisms that make up the food chain are directly and indirectly at risk with the effect of NPs. In this study, metallothionine (MT) biomarker responses were determined by exposing Gammarus pulex, an indicator species living in freshwaters, to zinc (Zn) and zinc oxide (ZnO) NPs. In the experiment, a total of four Zn and ZnO groups (Control, 10 ppm, 20 ppm, 40 ppm) including the control group were formed and studied with three replications. It was determined that Zn-NP application samples exposed to Cu-NP and taken at 24 and 96 hours caused an increase (p<0.05) in MT levels compared to the control. ZnO-NP application samples caused increases in MT levels in 96 hour samples compared to control (p<0.05), but there was no statistical difference in MT levels in 24th hour application samples (p<0.05). As a result of the analyzes made in the study, it is predicted that Zn and ZnO NPs cause an increase in MT levels in the organism, and this increase may cause oxidative stress. It has been determined that these nano-sized materials can be a big problem for the environment with the increasing usage areas.

Anahtar Kelimeler

• Metallotionein
• Gammarus pulex
• çinko nanopartikül
• çinkooksit nanopartikül

Kaynakça

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