EXPLORING BEE VENOM VOLATILES: A PROMISING AVENUE FOR CYSTIC FIBROSIS

Yıl 2024, , 267 - 284, 18.11.2024

Nilüfer Vural , Sibel Kaymak , Oğuz Yüce

https://doi.org/10.31467/uluaricilik.1520769

Öz

Bee venom, a complex mixture of bioactive compounds, has demonstrated anti-inflammatory, antimicrobial, and immunomodulatory properties. Notably, the volatiles released by bee venom components have garnered attention for their potential in respiratory-related disease conditions. Cystic fibrosis (CF) is a challenging disorder, characterized by a genetic mutation affecting the CFTR protein, leading to the production of thick and sticky mucus in various organs, particularly the lungs and digestive system, and necessitating innovative therapeutic approaches. This research explored both bee venom volatiles’ chemical composition and the effects on airway inflammation and mucus viscosity in CF patients by in silico methods. GC/MS analyses with various SPME fibers have conducted the identification of 67 distinct components in volatile compounds of bee venom. For CW/DVB, CAR-PDMS, and DVB-PDMS fibers, the compounds identified in the highest amounts were perilla alcohol (42.21%), tetradecane (11.48%), and 1,2-benzenedicarboxylic acid, 1,2-bis(2-methylpropyl) ester (39.98%), respectively. In silico analyses subsequently indicated that these components exhibit anti-inflammatory effects by modulating key cytokines and reducing inflammatory markers in CF airways. This research highlights the potential of bee venom volatiles as a novel therapeutic avenue for managing CF symptoms. Harnessing the unique properties of bee venom may offer new perspectives in the development of targeted therapies for individuals affected by cystic fibrosis.

Anahtar Kelimeler

Bee venom volatiles, Cystic fibrosis, SPME fibers, In silico

Etik Beyan

No experimentation on human or animal subjects was involved in this study. At the stage of bee venom sampling from honey bees, ethical permission is not required for insects. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Destekleyen Kurum

This research was supported by the Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agriculture Research and Policies (TAGEM) with Project number: TAGEM/21/AR-GE/25.

Teşekkür

The authors would like to thank Harun ARSLAN from Ankara Yıldırım Beyazıt University GETAT Center Research Apiary for his help in the care and supply of bees, and Turan ÇIRAK, technical staff of the GETAT Center, for his help in the special hive design.

Arı Zehri Uçucu Maddelerini Keşfetmek: Kistik Fibrozis İçin Umut Veren Bir Çözüm

Öz

Biyoaktif bileşiklerin karmaşık bir karışımı olan arı zehri, anti-inflamatuar, antimikrobiyal ve immünomodülatör özellikler göstermektedir. Özellikle arı zehri bileşenleri tarafından salınan uçucu bileşenler, solunum hastalıklarındaki potansiyelleri nedeniyle dikkat çekmektedir. Kistik fibroz (CF), CFTR proteinini etkileyen genetik bir mutasyonla karakterize, başta akciğerler ve sindirim sistemi olmak üzere çeşitli organlarda kalın ve yapışkan mukus üretimine yol açan ve yenilikçi tedavi yaklaşımları gerektiren zorlu bir hastalıktır. Bu araştırma, hem arı zehri uçucularının kimyasal bileşimini hem de CF hastalarında hava yolu inflamasyonu ve mukus viskozitesi üzerindeki etkilerini kimyasal ve in silico yöntemlerle araştırmıştır. Çeşitli SPME lifleri ile yapılan GC/MS analizleri, arı zehrinin uçucu bileşiklerinde 67 farklı bileşenin tanımlanmasını sağlamıştır. W/DVB, CAR-PDMS ve DVB-PDMS fiberleri için sırasıyla perilla alcohol (%42.21), tetradecane (%11.48) ve 1,2-benzenedicarboxylic acid, 1,2-bis(2-methylpropyl) ester (39.98) bileşenleri en yüksek miktarda belirlenmiştir. Daha sonra yapılan in silico analizler, bu bileşenlerin, temel sitokinleri modüle ederek ve CF hastalarının hava yollarındaki inflamatuar belirteçleri azaltarak anti-inflamatuar etkiler sergilediğini gösterdi. Bu araştırma, arı zehri uçucularının CF semptomlarını yönetmek için yeni bir terapötik yol olarak potansiyelini vurgulamaktadır. Arı zehrinin benzersiz özelliklerinden yararlanmak, kistik fibrozdan etkilenen bireylere yönelik hedefe yönelik tedavilerin geliştirilmesinde yeni bakış açıları sunabilir.

Anahtar Kelimeler

Arı zehri uçucu maddeleri, Kistik fibroz, SPME fiberi, In silico

Kaynakça

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