Endonezya Doğu Kalimantan'daki Calliandra calothyrsus plantasyonundan toplanan iğnesiz arı (Heterotrigona itama) balının antioksidan aktiviteleri ve fitokimyasal bileşimi

Yıl 2024, , 325 - 336, 18.11.2024

Swandari Paramita , Enos Tangke Arung , Syafrizal Syafrizal Netty Maria Naıbaho

https://doi.org/10.31467/uluaricilik.1539158

Öz

İğnesiz arı balı en değerli böcek ürünlerinden biridir. İğnesiz arı balının artan popülaritesi, tıbbi özelliklerle ilişkilendirilen bileşimine bağlanabilir. İğnesiz arılarla arıcılık Endonezya'da iyi bilinmektedir ve Heterotrigona itama, Endonezya'nın Doğu Kalimantan bölgesinde yetiştirilen en popüler iğnesiz arı türüdür. İğnesiz arılar, çeşitli bitki türlerini besin kaynağı olarak kullanırlar. Bu bitkiler arasında Calliandra calothyrsus, Endonezya arı plantasyonlarında popüler bir ekimdir. Bu çalışmada, C. calothyrsus plantasyonundan toplanan H. itama iğnesiz arı balının antioksidan (DPPH testi), fitokimyasal (kalitatif yöntem), su ve şeker içeriği analiz edilmiştir. Sonuçlar, bu çalışmadaki balın su ve şeker içeriğinin diğer araştırmalara göre daha yüksek olduğunu göstermektedir. Bu arada, antioksidan kapasitesi de diğer çalışmalara göre daha yüksek. Bu çalışmada baldan tespit edilen fitokimyasal içerikler karotenoidler, kumarinler, flavonoidler, saponinler, steroidler, tanenler ve triterpenoid. İğnesiz arı balının özellikleri bitki örtüsüne ve coğrafi kökene göre farklılık gösterebilse de, Endonezya'nın Doğu Kalimantan bölgesindeki C. calothyrsus plantasyonundan toplanan H. itama iğnesiz arı balı, insan sağlığı için avantajlı olan potansiyel antioksidan aktivite ve fitokimyasal içerik gösterir.

Anahtar Kelimeler

İğnesiz arı balı, Heterotrigona itama, Calliandra calothyrsus, DPPH, Fitokimyasal

Proje Numarası

KEP-74/BPDLH/2022

ANTIOXIDANT ACTIVITIES AND PHYTOCHEMICAL COMPOSITION OF STINGLESS BEE (Heterotrigona itama) HONEY COLLECTED FROM Calliandra calothyrsus PLANTATION IN EAST KALIMANTAN, INDONESIA

Öz

Stingless bee honey is one of the most valuable insect products. The increasing popularity of stingless bee honey can be attributed to its composition, which has been linked to medicinal properties. Beekeeping with stingless bees is well-known in Indonesia, with Heterotrigona itama is the most popular stingless bee species cultivated in East Kalimantan, Indonesia. Stingless bees utilize various plant species as sustenance sources. Among those plants, Calliandra calothyrsus is popular planting in Indonesian bee plantations. This study analyzed the antioxidant (DPPH assay), phytochemical (qualitative method), water, and sugar content of H. itama stingless bee honey collected from a C. calothyrsus plantation. The results show that the water and sugar contents of the honey in this study were higher than in other research. Meanwhile, antioxidant capacity was also higher than in other studies. The phytochemical contents detected from honey in this study were carotenoids, coumarins, flavonoids, saponins, steroids, tannins, and triterpenoids. Even though the properties of stingless bee honey can differ based on vegetation and geographical origin, H. itama stingless bee honey collected from C. calothyrsus plantation in East Kalimantan, Indonesia, showed potential antioxidant activity and phytochemical content, which is advantageous to human health.

Anahtar Kelimeler

Stingless bee honey, Heterotrigona itama, Calliandra calothyrsus, DPPH, Phytochemical

Etik Beyan

The authors declare that they have no competing interests

Destekleyen Kurum

Environmental Fund Management Agency, Ministry of Finance, Republic of Indonesia

Proje Numarası

KEP-74/BPDLH/2022

Teşekkür

This work is supported by the Fund for the Welfare and Sustainable Economy of Indigenous Peoples and Local Communities (Dana TERRA)

Kaynakça

• Abd Jalil MA, Kasmuri AR, Hadi H. Stingless bee honey, the natural wound healer: A review. Skin Pharmacol Physiol. 2017;30(2):66-75. doi.org/10.1159/000458416.
• Adalina Y, Kusmiati E, Pudjiani M. Phytochemical test and physical chemical properties of rubber honey from three types of bees (Apis mellifera, Apis dorsata and Trigona itama). IOP Conference Series: Materials Science and Engineering, IOP Publishing. 2020;935:012007. doi.org/10.1088/1757-899X/935/1/012007.
• Al-Hatamleh MA, Boer JC, Wilson KL, Plebanski M, Mohamud R, Mustafa MZ. Antioxidant-based medicinal properties of stingless bee products: Recent progress and future directions. Biomolecules. 2020;10(6):923. doi.org/10.3390/biom10060923.
• Al-Kafaween MA, Alwahsh M, Mohd Hilmi AB, Abulebdah DH. Physicochemical Characteristics and Bioactive Compounds of Different Types of Honey and Their Biological and Therapeutic Properties: A Comprehensive Review. Antibiotics. 2023;12:337. doi.org/10.3390/antibiotics12020337.
• Amin FAZ, Sabri S, Mohammad SM, Ismail M, Chan KW, Ismail N, et al. Therapeutic properties of stingless bee honey in comparison with European bee honey. Adv Pharmacol Pharm Sci. 2018;6179596, doi.org/10.1155/2018/6179596.
• Arung ET, Ramadhan R, Mandzilkh LD, Santoso PA, Matsumoto M, Nagata M, et al. Screening of selected stingless bee honey varieties for ACE2-spike protein-binding inhibition activity: A potential preventive medicine against SARS CoV-2 infection. Malays J Med Sci. 2022;29(5):146. doi.org/10.21315/mjms2022.29.5.15.
• Avila S, Beux MR, Ribani RH, Zambiazi RC. Stingless bee honey: Quality parameters, bioactive compounds, health-promotion properties and modification detection strategies. Trends Food Sci Technol. 2018;81:37-50, doi.org/10.1016/j.tifs.2018.09.002.
• Abu Bakar MF, Sanusi SB, Abu Bakar FI, Ong JC, Mian Z. Physicochemical and antioxidant potential of raw unprocessed honey from Malaysian stingless bees. Pak J Nutr. 2017;16(11):888-894. doi.org/10.3923/pjn.2017.888.894.
• Biluca FC, Braghini F, Gonzaga LV, Costa ACO, Fett R. Physicochemical profiles, minerals and bioactive compounds of stingless bee honey (Meliponinae). J Food Compost Anal. 2016;50:61-69, doi.org/10.1016/j.jfca.2016.05.007.
• Biluca FC, da Silva B, Caon T, Mohr ETB, Vieira GN, Gonzaga LV, et al. Investigation of phenolic compounds, antioxidant and anti-inflammatory activities in stingless bee. Food Res Int. 2020;129:108756. doi.org/10.1016/j.foodres.2019.108756.
• Biluca FC, de Gois JS, Schulz M, Braghini F, Gonzaga LV, Maltez HF, et al. Phenolic compounds, antioxidant capacity and bioaccessibility of minerals of stingless bee honey (Meliponinae). J Food Compost Anal. 2017;63:89-97. doi.org/10.1016/j.jfca.2017.07.039.
• Buchori D, Rizali A, Priawandiputra W, Raffiudin R, Sartiami D, Pujiastuti Y, et al. Beekeeping and managed bee diversity in Indonesia: Perspective and preference of beekeepers. Diversity. 2022;14(1):52. doi.org/10.3390/d14010052.
• Cardona Y, Torres A, Hoffmann W. Colombian stingless bee honeys characterized by multivariate analysis of physicochemical properties. Apidologie, 2019;50:881-892. doi.org/10.1007/s13592-019-00698-5.
• Cianciosi D, Forbes-Hernández TY, Afrin S, Gasparrini M, Reboredo-Rodriguez P, Manna PP, et al. Phenolic compounds in honey and their associated health benefits: A review. Molecules. 2018;23(9):1-20. doi.org/10.3390/molecules23092322.
• Food and Agriculture Organization (FAO). Codex Alimentarius: Standards for Honey CXS 12-1981. FAO, Rome, Italy, 2019.
• da Silva IAA, da Silva TMS, Camara CA, Queiroz N, Magnani M, de Novais JS, et al. Phenolic profile, antioxidant activity and palynological analysis of stingless bee honey from Amazonas, Northern Brazil. Food Chem. 2013;141(4):3552-3558. doi.org/10.1016/j.foodchem.2013.06.072.
• de Luna C, Calderon M, Cruz RV, Tolentino Jr E, Carandang W. The economic value of Calliandra calothyrsus in watershed rehabilitation in Manolo Fortich, Bukidnon, Philippines. J Environ Sci Manag. 2020;SI-2:76-84. doi.org/10.47125/jesam/2020_sp2/06.
• Department of Standards Malaysia. MS 2683 Malaysian Standards Kelulut (Stingless Bee) Honey-Specification. Department of Standards Malaysia, Kuala Lumpur, Malaysia, 2017.
• Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. J Exp Pharmacol. 2023;15:51-62. doi.org/10.2147/JEP.S379805.
• Fatima IJ, Mohd Hilmi AB, Salwani I, Lavaniya M. Physicochemical characteristics of Malaysian stingless bee honey from Trigona species. IIUM Med J Malays. 2018;17(1):187-191. doi.org/10.31436/imjm.v17i1.1030.
• Gulcin İ, Alwasel SH. DPPH Radical Scavenging Assay. Processes. 2023;11(8):2248. doi.org/10.3390/pr11082248.
• Harianja AH, Adalina Y, Pasaribu G, Winarni I, Maharani R, Fernandes A, et al. Potential of beekeeping to support the livelihood, economy, society, and environment of Indonesia. Forests. 2023;14(2):321. doi.org/10.3390/f14020321.
• Juliasih NKA, Arsana IN, Adi NNSP. Identifying feeding plants for Trigona sp. species in Royal Honey Sakah, Gianyar Regency, Bali. J Basic Sci Technol. 2022;11(3):93-106. doi.org/10.35335/jbst.v11i3.3479
• Kolaylı, S., Can, Z., Kara, Y., Ozkok, A., Ergin, S.O., Kemal, M. and Kanbur, E.D., 2024. Physicochemical characteristics, phenolic components, and antioxidant capacities of Lavender honey (Lavandula spp.) from Isparta region of Turkiye. Chem Biodivers. 2024;p.e202400718. doi.org/10.1002/cbdv.202400718.
• Kustiawan PM, Zulfa AF, Batistuta MA, Hanifa DNC, Setiawan IM. Comparative Analysis of Phytochemical, Total Phenolic Content, Antioxidant and Antibacterial Activity of Two Species Stingless Bee Propolis from East
• Kalimantan. Malays J Med Health Sci. 2022;18(SUPP10):50-55.
• Mahmood AL, Lani MN, Hassan Z, Razak SBA, Ahmad FT. Antioxidant and antimicrobial properties of Indo-Malayan stingless bee (Heterotrigona itama) honey from different seasons and distribution of flowers. Food Res. 2021;5(2):498-507. doi.org/10.26656/fr.2017.5(2).546.
• Maringgal B, Hashim N, Tawakkal ISMA, Mohamed MTM, Shukor NIA. Phytochemical compositions and antioxidant activities of malaysian stingless bee honey. Pertanika J Sci Technol. 2019;27(S1):15-28.
• Mwangi MW, Wanjau TW, Omwenga EO. Stingless bee honey: Nutritional, physicochemical, phytochemical and antibacterial validation properties against wound bacterial isolates. PLoS ONE. 2024;19(5):e0301201. doi.org/10.1371/journal.pone.0301201.
• Nasir NM, Mohd KS, Ibrahim N, Rodi MMM, Zakaria AJ. Phytochemical analysis and antioxidant activity of honey from three Malaysia stingless bee species. J Agrobiotechnology. 2019;10(2):1-11.
• National Standardization Agency of Indonesia (NSAI). SNI 01-2892-1992 Cara Uji Gula. NSAI, Jakarta, Indonesia, 1992.
• National Standardization Agency of Indonesia (NSAI). SNI 8664-2018 Madu. NSAI, Jakarta, Indonesia, 2018.
• Nordin A, Sainik NQAV, Chowdhury SR, Saim AB, Idrus RBH. Physicochemical properties of stingless bee honey from around the globe: A comprehensive review. J Food Compost Anal. 2018;73:91-102. doi.org/10.1016/j.jfca.2018.06.002.
• Omar S, Enchang FK, Nazri MUIA, Ismail MM, Ismail WIW. Physicochemical profiles of honey harvested from four major species of stingless bee (Kelulut) in North East Peninsular of Malaysia. Malays Appl Biol. 2019;48(1):111-116.
• Oscar SA, Antonio CN, Marina GV, Elsa RS, Gabriel VA. Phytochemical screening, antioxidant activity, and in vitro biological evaluation of leaf extracts of Hyptis suaveolens (L.) Poit. S Afr J Bot. 2020;128:62-66. doi.org/10.1016/j.sajb.2019.10.016.
• Rajkumar G, Panambara PAHR, Sanmugarajah V. Comparative Analysis of Qualitative and Quantitative Phytochemical Evaluation of Selected Leaves of Medicinal Plants in Jaffna, Sri Lanka. Borneo J Pharm. 2022;5(2):93-103. doi.org/10.33084/bjop.v5i2.3091.
• Rao A, Kumari S, Laura JS, Dhania G. Qualitative Phytochemical Screening of Medicinal Plants Using Different Solvent Extracts. Orient J Chem. 2023;39(3):621-626. doi.org/10.13005/ojc/390312.
• Rozman AS, Hashim N, Maringgal B, Abdan K. A comprehensive review of stingless bee products: Phytochemical composition and beneficial properties of honey, propolis, and pollen. Appl Sci. 2022;12(13):6370. doi.org/10.3390/app12136370.
• Saputra SH, Nurlina S. Chemical components, pollen and bioactivity of stingless honey bee kelulut (Heterotrigona itama) Meliponiculture from Samarinda, East Kalimantan. IOP Conference Series: Earth and Environmental Science, IOP Publishing 2022;985:012004. doi.org/10.1088/1755-1315/985/1/012004.
• Saputra SH, Saragih B, Kusuma IW, Arung ET. Antioxidant and antibacterial screening of honey of Heterotrigona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia. Nusantara Biosci. 2021;13(2):232-237. doi.org/10.13057/nusbiosci/n130213.
• Shamsudin S, Selamat J, Sanny M, Abd Razak SB, Jambari NN, Mian Z, et al. 2019. Influence of origins and bee species on physicochemical, antioxidant properties and botanical discrimination of stingless bee honey. Int J Food Prop. 2019;22(1):239-264. doi.org/10.1080/10942912.2019.1576730.
• Souza ECA, Menezes C, Flach A. Stingless bee honey (Hymenoptera, Apidae, Meliponini): A review of quality control, chemical profile, and biological potential. Apidologie. 2021;52:113-132. doi.org/10.1007/s13592-020-00802-0.
• Sujanto ISR, Ramly NS, Abd Ghani A, Huat JTY, Alias N, Ngah N. The composition and functional properties of stingless bee honey: A review. Malays J Appl Sci. 2021;6(1):111-127. doi.org/10.37231/myjas.2021.6.1.281.
• Sukemi S, Yasir Y, Paramita S, Arung ET. DPPH-Scavenging Activity of Propolis of Tetragonula iridipennis from East Kalimantan. Joint Symposium on Tropical Studies (JSTS-19), Atlantic Press, 2021;320-322. doi.org/10.2991/absr.k.210408.054
• Suliasih BA, Tawwabin RA, Budi S. Electroanalytical evaluation of antioxidant activity in monofloral honeys. Journal of Physics: Conference Series, IOP Publishing, 2021;1869:012051. doi.org/10.1088/1742-6596/1869/1/012051.
• Syafrizal S, Kusuma IW, Saud O, Wiandany R, Yahya M. Conservation of kelulut (stingless bee) in East and North Kalimantan, Indonesia. IOP Conference Series: Earth and Environmental Science, IOP Publishing, 2020;591:012047. doi.org/10.1088/1755-1315/591/1/012047.
• Syafrizal S, Ramadhan R, Kusuma IW, Egra S, Shimizu K, Kanzaki M, et al. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas. 2020;21(10):4623-4630. doi.org/10.13057/biodiv/d211021.
• Triwanto J, Herlinda K, Muttaqin T. Kualitas fisikokimia pada madu dari nektar bunga randu (Ceiba pentandra) dan kaliandra (Calliandra callothyrsus). J For Sci Avicennia. 2021;4(2):102-113. doi.org/10.22219/avicennia.v4i2.19750.
• Ucurum O, Tosunoglu H, Takma Ç, Birlik PM, Berber M, Kolaylı, S. Distinctive properties of the pine, oak, chestnut and multifloral blossom and honeydew honeys. Eur Food Res Technol. 2024;250(6):1765-1774. doi.org/10.1007/s00217-024-04520-0.
• Ustadi U, Radiati LE, Thohari I. Komponen bioaktif pada madu karet (Hevea brasiliensis) madu kaliandra (Calliandra callothyrsus) dan madu randu (Ceiba pentandra). Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK). 2017;12(2):97-102. doi.org/10.21776/ub.jitek.2017.012.02.6.
• Viji GS, Vasanthe B, Suresh K. Screening and antibacterial activity analysis of some important medicinal plants. Int J Innov Appl Stud. 2013;2(2):146-152.
• Ya’akob A, Norhisham NF, Mohamed M, Sadek N, Endrini S. Evaluation of physicochemical properties of Trigona sp. stingless bee honey from various districts of Johor. Jurnal Kejuruteraan. 2019;2:59-67. doi.org/10.17576/jkukm-2019-si2(1)-08.