The Effects Of Changing The Start Time Of The Light On Carbohyrate Levels On Gallaria Mellonella Adults At Different Ages

Yıl 2013, , 3 - 15, 12.06.2014

Yeşim Koç Adem Gülel

https://doi.org/10.31467/uluaricilik.162296

Öz

Goal: Protein,
lipid and carbohydrate metabolism plays role in many vital activities of insects. Adult insects need certain chemicals
and energy in large quantities to maintain vital activities such
as
mating, food searching, oviposition and parasitism. Therefore, having basal nutrients such as carbohydrates, lipids
and proteins in certain
quantities is a necessity. Necessary nutrients can be stored in larva or pupa stages or adults can synthesize them by taking related precursor compounds.
Some insects use carbohydrates as the primary source of energy and keep excess
carbohydrate as lipid and
protein. Generally Diptera and Hymenopera use carbohydrates as a main energy source. G. mellonella
is a harmful
species for combs and its larvae are known as causing
serious damages in beekeeping. There are not many studies available
regarding the effects of photoperiod, age and sex on carbohydrate levels of this insect. Since significant variations in carbohydrate levels can be observed
in connection with age and sex. Purpose of this study is to determine the effects on carbohyrate levels
on Gallaria mellonella adults at different age of changing
the start time of the light

Studies with effects on carbonhydrate levels of changing
the start time of light is insufficient. Studies on this insect usually
made with fixed
fotoperiod.

Materials ve Methods:

Large wax moth Galleria
mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) were used in experiments.
Studies were carried out
under the laboratory conditions at 28 ± 2°C temperature and

% 65 ± 5 relative humidity. Insects were reared at, six different photoperiod regimes eighteen hours light, six hours dark (18L; 6D) first level, (18L; 6D) second level, twelve hours light, twelve hours dark (12L; 12D) first level, (12L; 12D) second level,
six hours light,
eighteen hours dark (6L; 18D) first level,
(6L; 18D) second level). Illumination was done with

40 W fluorescent bulbs in different photoperiod regimes. It was made second level photoperiod regimes for 18L;6D, 6L; 18D, 12L; 12D under the condition different light on time. Insects were fed with honey free comb. Calculating the amount of carbohydrates for analysis, vanHandel (1985) Anthrone
test
was used.

One Way Analysis
of Variance (ANOVA) was used for the comparison of more than two groups.
Averages were assessed by using Student- Newman-Kuel (SNK) Test,
when the test results were significant. Independent Two Samples
t-test was employed for the comparison of two groups and
a = 0.05
confidence limit was taken
as
basis.

Discussion and conclusion: Photoperiod also affects the
amount of basic nutrients in
incects. The effect of photoperiod. İs clearly
seen in this study.
Some insects use carbohydrates as fuel primarily.
Under the same photoperiod regimes, there were increase amounts of carbohydrate in the first level photoperiod. Both females and and males, the amounts of carbohydrates increased as the adult aging. Increase of
dark period, caused decrease amounts     of     carbohydrates.     Amount of carbohydrate in some age groups,
no statistically significant differences, and while significant differences were found in some age groups.
This shows the importance of change the start time of light. The amount of carbohydrate in females is more than males, because females used in
the
food item,    carbohydrates    accumulate    more  
 and

synthesis.
and may be due to the consumption of more carbohydrate of male. Results from this study are show that changing
the
start time of light is very important. In this study with G. mellonella the relation between adult age and carbohydrate levels and age related gradual increase in carbohydrate level was observed. This increase might be in connection with the food as well as the inhibition of extreme carbohydrate consumption
in
the body due to Lepidoptera`s lipid preference as the primary
energy source. Increase
in the
carbohydrate level is not related to the glucose synthesis
from lipids because animals
do
not have the glyoxylate converting enzyme and glucose can not be synthesized from lipids. Besides, age related carbohydrate increase might be in connection with the components of the wax, which is the food, as they are employed
in glucose synthesis. Carbohydrate level of females, which is higher than males, can be linked to females ability of accumulating and synthesizing more carbohydrate from the food they were given and higher carbohydrate consumption of males. Training and production of Galleria mellonella
conditions of photoperiod and light revealed
the starting time should be
considered.

Anahtar Kelimeler

Galleria Mellonella, Photoperiod, Carbohydrate, Start Time Of The Light.

Aydınlık Başlangıç Zamanının Değişik Yaştaki Galleria Mellonella (Lepidoptera: Pyralidae) Erginlerinin Karbonhidrat Miktarı Üzerine Etkisi

Öz

Denemelerde büyük balmumu güvesi Galleria mellonella (Linnaeus,1758) (Lepidoptera: Pyralidae) kullanıldı. Çalışmalar, 28±2°C
ve %65±5 nispi nem içeren laboratuvar koşullarında yapıldı. Çalışmada kulla- nılan böcekler,
onsekiz saat aydınlık, altı saat karanlık (18A;6K); (1. Kademe) altı saat aydınlık, onsekiz saat karanlık (6A;18K); (1. Kademe),oniki saat aydınlık, oniki saat karanlık (12A;12K) (1. Kademe), onsekiz saat aydınlık, altı saat karanlık (18A;6K); (2. Kademe)altı saat aydınlık, onsekiz saat karanlık (6A;18K); (2. Kade- me),oniki
saat aydınlık, oniki saat karanlık (12A;12K) (2. Kademe) olmak üzere altı farklı fotoperiyot rejimi içeren ortamda yetiştirildi. Farklı fotoperiyot rejimlerinde, aydınlanma 40 W’lık floresan ampullerle sğlandı. Böceklerin
beslenmesinde, balsız petekler
kullanıldı.

Analizler
için karbonhidrat miktarının hesaplanmasında, van Handel’in
(1985) Anthrone testi kullanıldı. İkinci kademe şartlarında tutulan erginlerdeki karbonhidrat miktarı, 1. kademedekilerden daha düşük bulunmuştur. Ayrıca
her iki durumda, erginlerdeki karbonhidrat miktarının, yaş arttıkça
artığı gözlendi. Bütün fotoperiyot şartlarında, karbohidrat miktarının yaşla birlikte artığı saptandı. Genel olarak, uzun gün fotoperiyotlarında karbonhidrat miktarının kısa gün fotoperiyotlarındakinden, fazla olduğu görüldü.
Aynı fotoperiyot süreli, fakat aydınlanmanın başladığı zamanın farklı
olduğu fotoperiyotlarda, bazı yaş gruplarında karbohidrat miktarı
bakımından, istatistiksel olarak önemli farklar
ortaya çıkarken,
bazılarında fark önemsiz bulundu.

Anahtar Kelimeler

Galleria mellonella, Fotoperiyot, Karbonhidrat, Aydınlık Başlangıç Zamanı

Kaynakça

• Aktümsek, A., 1996. Parazitoid, Itoplectis maculator F. (Hymenoptera: Ichneumonidae)’un Yag Asiti Bileşimine Konak ve Eşey Farklılığının Etkisi. Tr. J. Of Zooll., 20, 7-10.
• Allemand, R., Cohet, Y., and Davis, J., 1973. Increase in The Longevity of Adult Drosophila melanogaster Kept in Permanent Darkness. Exp. Geront., 8, 279-283.
• Arai, T., 1999. Photoperiodic and Thermoperiodic Control of Hatching Time in Katydids. Entomol. Sci., 2, 4, 641-650.
• Ashley, N.T., Zhang, N., Weil, Z.M., Magalang, U.J. and Nelson, R.J. 2012. Photoperiod alters duration and intensity of non-REM sleep following immune challenge in Siberian hamsters (Phodopus sungorus). Chronobiology International.
• Atak, E. D., 1975. Fasülye Tohum Böcegi (Acanthoscelides obtectus) nın Tanınması ve Mücadelesi. T. C. Tarım Bakanlıgı Zirai Müca- dele ve Zirai Karantina Genel Müdürlügü, Mes- leki Nesriyat Serisi. 19, 8 s.
• Bailey, E., 1975. Biochemistry of Insect Flight. In ‘ Insect Biochemistry and Function’, (Ed. by. Candy, D.J. and Kilby, B. A.), Chapman and Hall. London. pp: 89-282.
• Barsagade, D. D., and Tembhare, D.B., 2002. Effect of Some Exogenous Factors on Silk Gland Protein in the Tropical Tasar Silkworm, Antherae mylitta. Ind. J. Ser., 41(1): 34-37
• Beck, S. D., 1963. Animal Photoperiodism: Relationship of Daylenght to Animal Growth, Development and Behaviour. Molt Library of Science Series-b, New York, 114 pp.
• Bogus, M.I., Wisniewski, J. R., and Cymborowski, B., 1987. Effect of Lighting Conditions on Endocrine Events in Galleria mellonella. J. Insect. Physiol., 33, 5, 355- 362.
• Bossard, R. L., Broce, A. B., and Dryden, M. W., 2000. Effects of Circadian Rhythms and Other Biossay Factors on Cat Flea Susceptility to Insecticides. J. of the Kansas Entomol. Soci., 73,1, 21-29.
• Bozkurt, K., 2003. Phospholipid and Triacylglycerol Fatty Acid Compositions from Varius Development Stages of Melanogryluss desertus Pall. (Orthoptera: Gryliidae). Türk. J. Biol., 27, 73-78.
• Büyükgüzel, K., 2002. Antimicrobial Agents: Their Combined Effects on Total Protein Content of the Endoparasitoid Pimpla Turionellae L. (Hymenoptera: Ichneumonidae). Türk. J. Zool., 26, 229-237.
• Canard, M., 1990. Effect of Photoperiod on the First Instar Development in the Lacewing Nineta pallida. Physiol. Entomol., 15, 137-140.
• Casanova-Ostos, R. A., 1992. Three Methods for Control of the Wax Month, Galleria mellonella, in Stored Honey Combs. Revista. Cientifi., 6,1, 5-16.
• Chang, C.P., and Hsieh, F. K., 1992. Morphology and Bionomics of Galleria mellonella L. Chinese J. Entomol., 12,121-129.
• Charriere, J. D., and Imdorf, A., 1999. Protection of Honey Combs From Wax Moth Damage. American Bee Journal.,139, 8, 627-630.
• Chloridis, A. S., Koveos, D. S., Stamopoulas, D. C., 1997. Effect of Photoperiod on the Induction and Maintenance of Diapause and Development of the Predatory Bug Podisus maculiventris. Entomophaga., 42,3, 427-434.
• Chocorosgui, V. R., and Panizzi, A. R., 2003. Photoperiod Influence on the Biology and Phenological Characteristics of Dichelops melacanthus (Heteroptera: Pentatomidae). Brazilian. J. Biol., 63,4, 655-664.
• Cymborowski, B., and Kryspin, I., 1975. The Effect of Photoperiod on K and Na Concentration in Larval and Pupal Haemolymph of Galleria mellonella. Acta. Entomologica. Bohemosl., 72,1, 13-18.
• Denlinger, D. L., 2002. Regulatıon of Diapause.Annu. Rev. Entomol., 47, 93-122.
• Deveci, Ö., 1996. Locusta migratoria cinerascens (Orthoptera: Acrididae)’in Menemen İzmir Ovası Populasyonunda Diyapozu Üzerine Araştırmalar. Tr. J. of Zooll., 20, 255- 259.
• El-Aw, M. A., 2003. Effect of Host Plant, Photoperiod, Day Time, Developmental Stage and Sex on Protein Patterns and Esterase Inhibition Heads of the Cotton Leafworm Spodoptera littoralis (Lepidoptera: Noctuidae). J. Agri. Res., 48,1, 89-52.
• England, S., and Evans, E. W., 1997. Effects ofPea Aphid (Homoptera: Aphididae) Honeydew on Longevity and Fecundity of the Alfalfa weevil (Coleoptera: Curculinoidae) Parasitoid Bathyplectes curculionis (Hymenoptera: Ichneumonidae). Environ. Entomol., 26, 1437-1441.
• Fantinou, A. A., Karandinos, M. G., and Tsitsipis, J. A., 1995. Diapause Induction in the Sesamia nonargioides (Lepidoptera: Noctuidae) Effect of Photoperiod and Temperature. Environ. Entomol., 24,6, 1458-1466.
• Fingerut, J. T., Zimmer, C. A., and Zimmer, R. K., 2003. Patterns and Processes of Larval Emergence in an Estuarine Parasite System. Biol. Bull., 205, 110-120.
• Fonken, L.K., Bedrosian, T.A., Michaels, H., Weil, Z.M., & Nelson, R.J. 2012. Short photoperiods attenuate central responses to an inflammogen. Brain, Behavior, and Immunity 26:617-622.
• Giron, D., and Casas, J., 2003. Lipogenesis in Adult Parasitic Wasp. J. Insect. Physiol., 49, 141- 147.
• Gürkan, F., 1985. Galleria mellonella’nın Populasyon Dinamigi. Doktora Tezi. H. Ü.
• Haewoon, O., ManYoung, L., and YoungDuck, C., 1995. Developing Periods and Damage Patterns of Combs by the Greater Wax Moth, Galleria mellonella. Korean. J. Apicult., 10,1, 5-10.
• Hartman, S. J., Menon, I., Collet, K. H., and Coley, N. J., 2001. Expression of Rhodopsin and Arrestin During the Light Dark Cycle in Drosophila. Molecular. Vision., 7,95-100.
• Hentz, M. G., Ellsworrth, P. G., Naranjo, S. E., and Watson, T. F., 1998. Development, Longevity and Fecundity of chelonus spp. Curvimaculatus (Hymenoptera: Braconidae) an Egg Larval Parasitoid of Pink Bollworm (Lepidoptera: Gelechiidae). Environ. Entomol., 27, 443-449.
• Higaki, M., and Ando, Y., 2003. Effects of Crowding and Photoperiod on Wing Morph and Egg Production in Eobiana engelhardti subtropica (Orthoptera: Tettigoniidae). App. Entomol. Zoo., 38,3, 321-325.
• Hodek, I., 1996. Diapause Development, Diapause Termination and the End of Diapause. Eur. J. Entomol., 93, 475-487.
• Hodkova, M., Berkova, P., and Zahradrickova, H., 2002. Photoperiodic Regulatıon of the Phospholipid Molecular Species Composition in Thoracic Muscles and Fat Body of Pyrrhocoris apterus (Heteroptera) Via an Endocrine Gland, Corpus Allatum. J. Insect. Physiol., 48,11, 1009-1019.
• Hong, J., KyeungSik, H., and KyungSaeng, B., 2003. Sex Pheromone of Aphis spira ecola (Homoptera: Aphididae): Composition and Circadian Rhythm in Release. J. Asia. Pas. Entomol., 6,2, 159-165.
• Hood, W., Horton, P. M., and McCreadie., J. M., 2003. Field Evaluation of the Imported Fire Ant (Hymenoptera: Formicidae) for the Control of Wax Moths (Lepidoptera. Pyralidae) in Stored Honey Bee Comb. J. Agri. Urban. Entomol., 20,2, 93-103.
• Horton, D. R., Hinojosa, T., and Olson, S. R., 1998. Effects of Photoperiod and Prey Type on Diapause Tendency and Preoviposition Period ın Perıllus bioculatus (Hemiptera: Pentatomidae) Can. Entomol., 130, 315-320.
• Izumi, Y., Anniwaer, K., Yoshida, H., Sanoda, S., Fujisaki, K., and Tsumuki, H., 2005. Comparison of Cold Hardiness and Sugar Between Diapausing and Nondiapausing Pupae of the Cotton Bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Physiol. Entomol., 30, 36-41.
• Kawazu, K., Adati, T., and Tatsuki, S., 2003. Effects of Photoregime on Timing of Male Responses to Sex Pheromones in Male Cnaphalocrocis medinalis (Lepidoptera: Crambidae). App. Entomol. Zool., 38,3, 327-331.
• Kimura, Y., and Masaki, S., 1992. Effect of Light Period on Dark Time Measurement for Diapause Induction in Mamestra brassicae. J. Insect. Physiol., 38, 9, 681-686.
• Kingsolver, J. G., and Woods, H. A., 1998. Interactions of Temperature and Dietary Prote- in Concentration in Growth and Feeding of Manduca sexta Caterpillars. Physiol. Entomol., 23, 354-359.
• Kimura, M. T., 1990. Quantitative Response to Photoperiod During Reproductive Diapause in the Drosophila auraria Species- Complex. J. Insect. Physiol., 36, 3, 147- 152
• Kryspin, I., Dutkowski, A. B., and Cymborowski, B., 1974. The Influence of Wllumination Conditions on Growth and Development of Galleria mellonella. Bull. Acad. Pol. Sci., 22, 803-808.
• Macedo, L. P. M., Souza, B., Carvaiho, C. F., and Ecole, C. C., 2003. Influence of the Photoperiod on Development and Reproduction of Chrysoperia externa (Neuroptera: Chrysopidae). Neutropical. Entomol., 32,1, 91-96.
• Linne, C, 1758. Systema Naturae, Vol.,1,10th edit
• Meats, A., and Leighton, M., 2004. Protein Consumption by Mated, Unmated, Sterile and Fertile Adults the Queensland Fruit Fly, Bactrocera tryoni and It’s Relation to Egg Production. Physiol. Entomol., 29, 176-182.
• Mellini, E., and Dindo, M. L., 1982. Effects of Photoperiod on the Immature Stages of the Host Parasite Couple Galleria mellonella L. Gonia cinerascens. Rond. Bollettino. Dell. Inst. Di. Ent. Del. Uni. Delgi. St. Bologna., 36, 114- 131.
• Moribayashi, A., Kurahashi, H., Sugie, H., Katagiri, C., and Tsuchida, K., 2003. Lipids and Ecdysteroids on the Ovarian Growth of the Adult Blow Fly, Aldrichina grahami. Med. Ent. Zool., 54,1, 65-71.
• Morita, A., Soga, K., Hoson, T., Kamisaka, S., and Numata, H., 1999. Changes in Mechanical Properties of the Cuticle and Lipid Accumulation in Relation to Adult Diapause in the Bean Bug, Riptortus clavatus. J. Insect. Physiol., 45,3, 241-247
• Nakasuji, F., and Mizumoto, M., 2001. Morphological and Physiological Traits of Seasonal Forms of a Migrant Skipper, Parnara guttata guttata (Lepidoptera: Heaperiidae). Spe. Pub. Japan. Coleop. Socrety., 1, 45-54.
• Nealis, V. G., Oliver, D., and Tcir, D., 1996. The Diapause Response to Photoperiod in Ontaria Populations of Cotesia melanoscela (Hymenoptera: Braconidae). Can. Entomol., 128, 41-46.
• Niva, C. C., and Takeda, M., 2003. Effects of Photoperiod, Temperature and Melatonin on Nymphal Development, Polypherism and Reproduction in Halyomorpha halys (Heteroptera: Pentatomidae). Zool. Sci., 20,8, 963-970.
• Nunes, M. V., and Hardie, J., 1992. Photoperiodic Induction of Winged Females in the Black Bean Aphid, Aphis fabae. Physiol. Entomol., 17, 391-396.
• Olson, D. M., and Andow, D. A., 1998. Larval Crowding and Adult Nutrition Effects on Longevity and Fecundity of Female Trichogramma nubilale Ertle and Davis (Hymenoptera: Trichogrammatidae). Environ. Entomol., 27, 508-514.
• Olson, D. M., Fadamıro, H., Lundgren, J. G., and Heimpel, G. E., 2000. Effects of Sugar Feeding on Carbohydrate and Lipid Metabolism in a Parasitoid Wasp. Physiol. Entomol., 25, 17-25.
• Özalp, P., ve Emre, _., 1998. Karbohidratların Pimpla Turionellae L. Ergin Dişilerinde Total Glikojen ve Protein Miktarına Etkileri. Tr. J. of. Biol., 22, 15-19.
• Philips, J. G., 1975. Enviromental Physilogy. Blackwell. Sci. Pub., Oxford-London. 131-149.
• Pullin, A. S., and Wolda, H., 1993. Glycerol and Glucose Accumulation During Diapause in a Tropical Beetle. Physiol. Entomol., 18, 75-78.
• Ramadan, M. M., Wong, T. T. Y., and Messing, R. H., 1995. Reproductive Biology of Biosteres vandenboschi (Hymenoptera: Braconidae), a Parasitoid of Early Instar Oriental Fruit Fly.Ann. Entomol. Soc. Am., 88,2, 189-195.
• Saunders, D. S., 1997. Under Sized Larvae From Short Day Adults of the Blow Fly, Calliphora vicina, Side- Step the Diapause Programme. Physiol. Entomol., 22, 249-255.
• Saunders, D. S., Lewis, R. D., and Warman, G. R., 2004. Photoperiodic Induction of Diapause: Opening the Black Box. Physiol. Entomol., 29, 1-15.
• Shuxia, Y. Y., and Adams, T. S., 2000. Effect of Pyriproxyfen and Photoperiod on Free Amino acid Concentrations and Proteins in the Hemolymph of Colorado Patato Beetle, Leptinotarsa decemlineata. J. Insect. Physiol., 46,10, 1341-1353.
• Socha, R., and Sula, J., 1992. Voltinism and Seasonal Changes in Haemolymph Protein Pattern of Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) in Relation to Diapause. Physiol. Entomol., 17, 370-376.
• Şeker, D. A., ve Yanıkoglu, A., 1999. Pimpla turionellae L. (Hymenoptera: Ichneumonidae)’ nın Açlık, Beslenme, Parazitleme ve YaslılıkDurumlarında Glikojen Seviyesindeki Degismeler. Tr. J. of. Zool., 23, 289-296.
• Thompson, J. J. W., Armitage, S. A. O., and Jothy, M. T. S., 2002. Cuticular Colour Change After Imaginal Eclosion is Time-Constramed: Blacker Beetles Darken Faster. Physiol. Entomol., 27, 136-141.
• Van Handel, E., 1985. Rapid Determination of Total Lipids in Mosguitoes, J. Am. Mosg. Cont. Assoc., 1, 302-304.
• Walton, J.C., Chen, Z., Weil, Z.M., Pyter, L.M., Travers, J.B. & Nelson, R.J .2011. Photoperiod-mediated impairment of long-term potentiation and learning and memory in male white-footed mice. Neuroscience, 175:127- 132.
• Warburg, M. S., and Yuval, B., 1996. Effects of Diet and Activity on Lipid Levels of Mediterranean Fruit Flies. Physiol. Entomol., 21, 151-158.
• Wassmer, G. I., Cain, W., and Page, T. L., 1996. Photoperiodic Regulation of Hemolymph Pro- tein in the Woodroach Parcoblatta pennsylvanica. J. Insect. Physiol., 42,9, 851-858.
• Watari, Y., 2003. Phase Shifting Effects of a Light Pulse Interrupting Scotophase on Locomotor Activity Rhythm and Photoperiodic Time Measurement for Diapause in the Onion Fly, Delia antigua. Entomol. Sci., 6,4, 229-235.
• Weber, H., Weidner, H., 1974. Grundrins Der Wnsekten Kunde. Gustav Fischer Verlong, Stuttgard, 1-640.
• Workman, J.L., Manny, N., Walton, J.C., & Nelson, R.J. 2011. Short day lengths alter stress and depressive-like responses, and hippocampal morphology in Siberian hamsters. Hormones and Behavior, 60:520-528.
• Yacobovitch, T., Benayahu, Y., and Weis, V. M., 2004. Motility of Zooxanthella Isolated From the Red Sea Soft Carol Heteroxenia fuscescens. J. Exp. Marine Biol. Ecol., 298, 35-48.
• Yanıkoglu, A., 1985. Schistocerca gregaria Forskal ( Ortheptera: Acrididae ) Nimflerinin Dogal ve Sentetik Besinde Gelisimi Sırasında Glikojen Miktarı Tayini. Doga Bilim Dergisi., A2, 9,3, 582-592.
• Zaslavski, V. A., 1992. Light Break Experiments with Emphasis on the Quantitative Perception of Nightiength in the Aphid Megoura vicia. J. Insect. Physiol., 38, 9, 717- 725.