Thiamethoxam’ın Yapay Çiçeklerde Seçenekli Olarak Verildiğinde Yayılmacı Arıların Davranışı Üzerindeki Etkileri

Yıl 2018, , 2 - 13, 28.05.2018

İbrahim Çakmak

https://doi.org/10.31467/uluaricilik.427583

Cited By: 2

Öz

Mavi ve
beyaz yapay çiçekleri ziyaret eden yayılmacı arıların davranışı üzerinde
thiamethoxam etkileri çalışılmıştır. Thiamethoxam dozları %2 -% 40 ‘e kadar
rapor edilen LD50 değerleri olarak verilmiştir. 
Çalışma kesintisiz olarak uygulanan test fazı 3 kısımdan oluşmaktadır.
Ilk kısımda arılar 6 ul ve 1 M her iki renk çiçeklerde sukroz verdik.Ikinci
kısımda arılara 6 ul ve 1.5 M şeker solüsyonu mavi çiçeklerde 6 ul ve 0.5 m
şeker solüsyonu beyaz çiçeklere verdik.Üçünü kısımda ise arılara çiçek rengine
göre ikinci kısımın tam tersi olarak şeker solüsyonu verdik.Her çalışma arılara
ve çiçeklere yayılışları insektisitler verdikte 30 dk sonra
başlamıştır.Yayılmacı arıların sayıları, her çiçeğe ziyaret sayısı, çiçek
tercihleri ve çiçeklere geri dönmeyen arılar kayıt edilmiştir.Yayılmacı
arıların çiçeklere geri dönüş seviyeleri artan thiamethoxam dozu ile
azalmıştır.Çalışmada kullanılan toplam 96 arıdan 50 arı yüksek doz
thiaamethoxam verilen guruba aittir.Çiçeklere geri dönen yayılmacı arıların
ziyaret sayıları olumsuz etkillenmiştir.Fakat arıların çiçek bağımlılığı
thiamethoxam dozundan etkilenmemiştir. Beyaz ve mavi çiçekleri çalışmanın ilk
kısmında çok sayıda ziyaret eden yayılmacı arılar ikinci kısımda daha koyu
şekeri olan ödül olan çiçeğe ziyaret etmişler ve üçüncü kısımda ise daha koyu
şeker solüsyonu olan çiçekelere ziyaret etmeyi öğrenememişlerdir. Sonuç olarak
bu çalışma göstermiştirki thimaethoxam çiçeklere geri dönen arıların sayısı,
çiçeklere ziyaret sayısı ve ödülün yayılmacı arılar tarafından yeniden
öğrenilmesini etkilemiştir.

Anahtar Kelimeler

Apis mellifera, Bal Arısı, Yayılma Davranışı, Thiamethoxam, Neonicotinoids

EFFECTS OF THIAMETHOXAM ON THE BEHAVIOR OF FORAGING HONEY BEES WITH ARTIFICIAL FLOWER CHOICES

Öz

The
effects of thiamethoxam were studied on the foraging behavior of free-flying
bees (Apis mellifera anatoliaca) visiting
artificial flower patches of blue and white flowers. Thiamethoxam doses from 2
% to 40 % of the reported LD₅₀ value were given to bees. The study
consisted of three experimental parts performed sequentially without
interruption. In part 1, we offered bees 6 μL of a 1M sucrose reward in both
flower colors.  In part 2 we offered bees
6 μL of 1.5 M sucrose solution in blue flowers and 6 μL of 0.5 M sucrose
solution in white flowers.  In part 3 we
reversed the sucrose solution rewards values with respect to flower color.  Each experiment began 30 min after
administration of the insecticide. The number of bees foraged was recorded, as
was flower patch visitation rate, number of flowers visited and flower choices
of the bees that did return. The forager return rate declined linearly with
increasing thiamethoxam dose and number of foraging trips of returning bees was
also affected adversely. Out of 96 bees, the majority of unreturned (50) bees
belonged to higher dosages of thiomethoxam groups. However, flower fidelity was
not affected by thiamethoxam dose. Foragers visited both blue and white flowers
extensively in experimental part 1 and showed greater fidelity for the flower
color offering the higher molarity reward in parts 2 but there were less visits
to flowers offering the higher molarity reward in part 3 indicating that the
bees failed to learn what were the flowers with higher reward. Our study showed
that thiamethoxam affected: the number of returning bees, the number of foraging
trips and reward re-learning.

Anahtar Kelimeler

: Apis mellifera, Honey Bee, Foraging Behavior, Thiamethoxam, Neonicotinoids

Kaynakça

• Aliouane Y, el Hassani AK, Gary V, Armengaud C, Lambin M, Gauthier M (2009) Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environmental Toxicology and Chemistry 28:113-122.
• Arena M., Sgolastra F (2014) A meta-analysis comparing the sensitivity of bees to pesticides.Ecotoxicology 23:324-334. Bonmatin JM, Marchand PA, Charvet R, Moineau I, Bengsch ER, Colin ME (2005) Quantification of imidacloprid uptake in maize crops. J Agric Food Chem 53:5336–5341.
• Bryden J, Gill RJ, Mitton RAA, Raine NE, Jansen VAA (2013) Chronic sublethal stress causes bee colony failure. Ecology Letters 16:1463-1469.
• Charpentier G, Louat F, Bonmatin J-M, Marchand PA, Vanier F, Locker D, Decoville M (2014) Lethal and sublethal effects of imidacloprid, after chronic exposure, on the insect model Drosophila melanogaster.Environmental Science & Technology 48:4096-4102.
• Cutler GC, and CD Scott-Dupree 2007. Exposure to clothianidin seed-treated canola has no long term impact on honey bees. Journal of Economic Entomology100: 765-772.
• Colin ME, Bonmatin JM, Moineau I, Gaimon C, Brun S, Vermandere JP (2004) A method to quantify and analyze the foraging activity of honey bees: Relevance to the sublethal effects induced by systemic insecticides. Archives of Environmental Contamination and Toxicology 47:387-395.
• Cressey D (2013) Europe debates risk to bees. Nature 496:408-408.
• Cresswell JE (2011) A meta-analysis of experiments testing the effects of a neonicotinoid insecticide (imidacloprid) on honey bees.Ecotoxicology 20:149-157.
• Cresswell JE, Desneux N, vanEngelsdorp D (2012a) Dietary traces of neonicotinoid pesticides as a cause of population declines in honey bees: An evaluation by Hill's epidemiological criteria. Pest Management Science 68:819-827.
• Cresswell JE, Page CJ, Uygun MB, Holmbergh M, Li Y, Wheeler JG, Laycock I, Pook CJ, de Ibarra NH, Smirnoff N, Tyler CR (2012b) Differential sensitivity of honey bees and bumble bees to a dietary insecticide (imidacloprid). Zoology 115:365-371.
• Laurino, D., Manino, A., Patetta, A., Porporato, M. (2013)Toxicity of neonicotinoid insecticides ondifferent honey bee genotypes. Bulletin of Insectology 66 (1): 119-126.
• Decourtye A, Lacassie E, Pham-Delegue MH (2003) Learning performances of honeybees (Apis mellifera L) are differentially affected by imidacloprid according to the season. Pest Management Science 59:269-278.
• Decourtye, A., Devillers, J., Cluzeau, S., Charreton, Pham-Dele`guea, MM. (2004) Effects of imidacloprid and deltamethrin on associative learning in honeybees under semi-field and laboratory conditions.Ecotoxicology and Environmental Safety. 57: 410–419.
• DeGrandi-Hoffman G, Sammataro D, Simonds R (2012) Are agrochemicals present in high fructose corn syrup fed to honey bees (Apis mellifera L.) Journal of Apicultural Research 51:371-372.
• Feltham H, Park K, Goulson D (2014). Field realistic doses of pesticide imidacloprid reduce bumblebee pollen foraging efficiency. Ecotoxicology 23:317-323.
• Fischer J, Muller T, Spatz AK, Greggers U, Grunewald B, Menzel R (2014) Neonicotinoids interfere with specific components of navigation in honeybees. Plos One 9. doi:10.1371/journal.pone.0091364.
• Gill RJ, Ramos-Rodriguez O, Raine NE (2012) Combined pesticide exposure severely affects individual- and colony-level traits in bees. Nature 491:105-119.
• Girolami V, Mazzon L, Squartini A, Mori N, Marzaro M, Di Bernardo A, Greatti M, Giorio C, Tapparo A (2009) Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: A novel way of intoxication for bees. Journal of Economic Entomology 102:1808-1815.
• Hassani AK, Dacher M, Garry V, Lambin M, Gauthier M, Armengaud C. (2008) Effects of sublethal doses of acetamiprid and thiamethoxam on the behavior of the honeybee (Apis mellifera).Arch Environ Contam. Toxicol.54:653–661.
• Henry M., Beguin M., Requier, F., Rollin O., Odoux, JF., Aupinel, P., Aptel, J., Tchamitchian, S., Decourtye, A. (2012) A Common Pesticide Decreases Foraging Success and Survival in Honey Bees. Science, 336: 348-350.DOI:10.1126/science.121503.
• Jeschke, P., Nauen, R., Schindler, M., Elbert, A. (2011) Overview of the Status and Global Strategy for Neonicotinoids. J. Agric. Food Chem. 2011, 59, 2897–2908, doi.org/10.1021/jf101303g.
• Karahan, A. Çakmak, I., Hranitz, J.M., Karaca, I., and Wells, H. (2015). Sublethal Imidacloprid effects on honey bee flower choices when foraging. Ecotoxicology, 24, 2017-2025.
• Kandemir I, Kence M, Sheppard WS and Kence A (2006) Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey. J Apicul Res45:33-38.
• Kandemir I, Kence M, Kence A. (2000) Genetic and morphometric variation in honey bee (Apis mellifera L.) population of Turkey.Apidology 31:343-356.
• Kevan P. (1975) Forest application of the insecticide Fenitrothion and its effect on wild bee pollinators (Hymenoptera: Apoidea) of lowbush blueberries (Vaccinium spp.) in southern New Brunswick, Canada. Biological Conservation 7:301-309.
• Lu C, Warchol KM, Callahan RA (2014) Sublethal exposure to neonicotinoids impaired honey bees winterization before proceeding to colony collapse disorder. Bulletin of Insectology 67:125-130.
• Matsumoto, T. (2013). Reduction in homing flights in the honey bee Apis mellifera after a sublethal dose of neonicotinoid insecticides. Bulletin of Insectology 66 (1): 1-9.
• Maus, C., Cure, G., Schmuck, R. (2003) Safety of imidacloprid seed dressings to honey bees: a comprehensive overview and compilation of thecurrent state of knowledge. Bulletin of Insectology 56 (1): 51-57.
• Mullin CA, Frazier M, Frazier JL, Ashcraft S, Simonds R, et al. (2010) High levels of miticides and agrochemicals in North American apiaries: Implications for honey bee health. PLoS ONE 5: e9754.
• Neumann, P., Carreck, N. (2010) Honey bee losses. Journal of Apicultural Research.49 (1): 1-6.
• Ramirez-Romero R, Chaufaux J, Pham-Delegue MH (2005) Effects of Cry1Ab prototoxin, deltamethrin and imidacloprid on the foraging activity and the learning performances of the honeybee, Apis mellifera: a comparative approach. Apidologie 36:601-611.
• Rortais A, Arnold G, Halm MP, Touffet-Briens F (2005) Modes of honeybees exposure to systemic insecticides: Estimated amounts of contaminated pollen and nectar consumed by different categories of bees. Apidologie 36:71-83.
• Ruttner F (1988) Biogeography and Taxonomy of Honey-bees. Springer-Verlag, Berlin. Ruttner, F. (1992) Naturgeschichte der honigbienen. Ehrenwirth, München, 357 pp.
• Prisco, GD., Cavaliere, V., Annoscia, d., Varricchio, p., Caprio, E., Nazzi, F., Gargiulo, G., Pennacchio, F. (2013) Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees. PNAS18466–18471,doi: 10.1073/pnas.1314923110.
• Oldroyd B. P., (2007) What’s killing American honey bees? Plos Biology, 5: 1195-1199.
• Osborne, JL. (2012) Ecology: Bumble bees and pesticides. Nature 491: 43-45.
• Schmuck R., Schoning R., StorkAa., Schramel O., (2001) Risk posed to honeybees (Apis mellifera L., Hymenoptera) by an imidacloprid seed dressing of sunflowers. Pest Management Science, 57 (3): 225-238.
• Scholer J, Krischik V (2014) Chronic exposure of imidacloprid and clothianidin reduce queen survival, foraging, and nectar storing in colonies of Bombus impatiens.Plos One 9 doi:10.1371/journal.pone.0091573.
• Schneider, C. W.,Tautz, J.,Grunewald, B. & Fuchs, S. (2012). RFID tracking of sublethal effects of two neonicotinoid insecticides on the foraging behavior ofApis mellifera. PLoS ONE 7,e30023.
• Stoner KA, Eitzer BD (2012) Movement of soil-applied imidacloprid and thiamethoxam into nectar and pollen of squash (Cucurbita pepo).PLoS One 7.doi:10.1371/journal.pone.0039114
• van Engelsdorp, D, Hayes, J., Underwood, RM, Pettis, J.S. 2010. A survey of honey bee colony losses in the United States, fall 2008 to spring 2009. Journal of ApiculturalResearch 49:7-14.
• van Engelsdorp, D., J. Hayes, R. M. Underwood, D. Caron and J. S. Pettis. 2011A survey of honey bee colony losses in the United States,fall 2009 to spring 2010.Journal of Apicultural Research. 50: 1-10
• Yang, Y., Monine, M., Faeer, JR., Hlavacek, WS.(2008). Kinetic Monte Carlo method for rule-based modeling of biochemical networks.Physical Review E. DOI: 10.1103/PhysRevE.78.
• Williamson SM, Willis SJ, Wright GA (2014) Exposure to neonicotinoids influences the motor function of adult worker honeybees. Ecotoxicology.DOI 10.1007/s10646-014-1