PHENOLIC COMPOSITION AND ANTIOXIDANT PROPERTIES OF ANZER HONEY FROM BLACK SEA REGION OF TURKEY

Yıl 2019, Cilt: 19 Sayı: 2, 143 - 151, 12.11.2019

Meltem Malkoç , Hilal Çakır , Yakup Kara Zehra Can , Sevgi Kolaylı

https://doi.org/10.31467/uluaricilik.602906

Cited By: 16

Öz

Anzer honey is produced Turkey's famous Anzer Plateau with rich vegetation in the Eastern Black Sea region.This is the first extensive investigation of the bioactive characteristics of Anzer honey. For this purpose, melissopalinological analysis, total phenolic and flavonoid substances, polyphenolic profile and total antioxidant capacity of honey, ferric reducing antioxidant power test (FRAP) and free radical scavenging capacity of samples were measured by picryhydrazil (DPPH) method. According to the results of the study, the total phenolic content of Anzer honey was 26.92 gallic acid/100g and the total flavonoid substance was 2.79. In the analysis of phenolic compounds of the Anzer honeys, daidzein and syringic acid were not detected. The major phenolic compounds in the honey were, in descending order, pinocembrin, hesperidin, chrysin, protocatechuic acid, p-coumaric acid, catechin, caffeic acid phenyl acid (CAPE), p-OH benzoic acid, caffeic acid, and  minor levels of myricetin, luteolin, rutin, resveratrol, epicatechin, t-cinnamic acid,  ferulic acid and gallic acid, were also determined.  The mean FRAP and DPPH values of the anzer honeys were found to be 110.11 μmolTrolox/100g, and 49.12mg/ml  respectively. As a result, although Anzer honeys are not rich in phenolic contents, they are attractive in terms of phenolic compounds.

TÜRKİYE'NİN KARADENİZ BÖLGESİNDEN ANZER BALININ FENOLİK BİLEŞİMİ VE ANTİOKSİDAN ÖZELLİKLERİ

Öz

Anzer balı, Türkiye'nin Doğu Karadeniz bölgesinde zengin bitki örtüsü ile ünlü Anzer platosunda üretilmektedir. Anzer balı dünyaca tanınan bir bal olup, tıbbi değerinin yüksek olduğuna inanılmaktadır. Bu çalışma Anzer balının biyoaktif özelliklerinden sorumlu polifenolik profilinin belirlendiği melissopalinolojik analiz, toplam fenolik madde, toplam flavonoid madde ve toplam antioksidan aktivitelerinin tespit edildiği ilk detaylı araştırmadır. Antioksidan aktivite demir (III) indirgeyici antioksidan güç testi (FRAP) ve 1,1-difenil-2-pikrilhidrazil (DPPH) serbest radikali temizleme testi ile ölçüldü. Anzer balının toplam fenolik içeriğinin ortalama 26.92 GAE/100g ve toplam flavonoid madde miktarının 2.79 mg QE/100g olduğu tespit edildi. Anzer ballarının ortalama FRAP ve DPPH değerleri sırasıyla 110,11 μmolTrolox/100g ve 49,12 mg/mL olarak bulundu. Anzer balının fenolik asitleri ve flavonoidleri yüksek performanslı sıvı kromatografisi (HPLC) ile belirlendi. Analizde kullanılan 19 adet standart bileşikten daidzein ve şiringik asit hariç, pinosembrin, hesperidin, krisin, protokatekuik asit, p-kumarik asit, kateşin, kafeik asit fenil ester (CAPE), p-OH benzoik asit, kafeik asit major bileşen, mirisetin, luteolin, rutin, resveratrol, epikateşin, t-sinnamik asit, ferulik asit ve gallik asit ise minor bileşen olarak tespit edildi. Bu sonuçlara göre, Anzer balı içerdiği polifenolik maddelerin çeşitliliği bakımından dikkat çekici özelliğe sahip bir çiçek balıdır. 

Anahtar Kelimeler

Anzer honey, antioxidant, phenolics

Kaynakça

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