Mikro-element içeren şeker solusyonu ile beslenen işçi arıların kanında ağır metallerin miktarındaki değişiklikler

Yıl 2004, Cilt: 04 Sayı: 2, 77 - 80, 20.05.2004

Ivanka Zhelyazkova Margarita Marınova Kalinka Gurgulova

Öz

Bu çalışma birbirine eşitlenmiş Dadant tipi 6 koloni üzerinde yapılmıştır. Bu kolonilerin 3’ü kontrol olup ağır metaller eklenmeden şeker solusyonu ile, deneme grubunda ise 3 koloniye 1,5 ppm Pb, 1,5 ppm Cd, 2,0 ppm Cu, 50,0 Zn, (0,0 ppm Fe, 20,0 ppm Mn, 20,0 Co içeren şeker solusyonu verilmiştir. Deneme sonucunda (besleme bittikten sonra) arıların kan örnekleri alınmıştır. Đçinde Pb, Cd, Cu, Zn, Mn, ve Fe içeren şeker solusyonları ile besleme yapılan işçi arıların kanında bu elementlerin miktarında artış görülmüştür. Yüzde olarak en büyük değişiklik kontrol grubuna göre sırasıyla Mn, Cd, Pb, 21, 7, 17, ve 8,0 kez daha fazla görülmüştür. Cd aslında besinde en az konsantrasyonda olan elementler arasındadır. Bu sonuçlara dayanarak yazar, çevrede ağır metallerin seviyesinin belirlenmesinde ağır metallerin arıların kanında sürekli birikiminin gözlenmesinin bir gösterge olabileceğini önermektedir.

Anahtar Kelimeler

Bal arıları, ağır metaller, hemolimf, şeker solusyonu, biyolojik gösterge

Changes in the Quantity of Heavy Metals in the Haemolymph of Worker Bees Fed Micro-element Contaminated Sugar Solution

Öz

The study was initiated by making 6 colonies (set in 10-frame Dadan Blatt bee-hives) equal by the method of analogues. The bees in the control group (3 colonies) were fed sugar solution without heavy metal additives, while those in the experimental group (3 colonies) were fed sugar solution containing 1,5 ppm Pb, 1,5 ppm Cd, 2,0 ppm Cu, 50,0 ppm Zn, 80,0 ppm Fe, 20,0 ppm Mn, 20,0 ppm Co. At the end of the experimental period (after the end of feeding) haemolymph samples were taken. After feeding the bees with sugar solution contaminated with Pb, Cd, Cu, Zn, Mn, Co and Fe the quantity of all of these elements in the haemolymph of workers increased. The greatest percentage changes occurred in the levels of Mn, Cd and Pb (respectively 21,7 , 17,7 and 8,0 times higher than the control group). However, Cd was among the elements in lowest concentration in the food. On the basis of these results, the authors think that the haemolymph of bees shows promise for biomonitoring heavy metals in the environment (as an accumulative indicator).

Anahtar Kelimeler

Honey bees, heavy metals, haemolymph, sugar solution, bio-indicator

Kaynakça

• Billalov F.S., B.I. Kolupaev, Yu.S. Kotov, S.S. Muharamova, L.А. Srebneva, 1992, Bee products and environmental control, Pchelovodstvo, 12, 4-6
• Bromenshek J., 1986, Bees as indicators of pollution in environment, Gleanings in bee culture, 114, 1, 19
• Goloskokov V.G., P.К. Pimenov, 1972, Metabolism of microelements in bees, Pchelovodstvo, 12, 35.
• Grigoryan G., 1970, Microelements in the haemolymph of bees, Pchelovodstvo, 3, 38.
• Grigoryan G., 1972, Parameters of the haemolymph of larvae, Pchelovodstvo, 1, 39.
• Hsu Yuan, Chia Welli, 1993, The ultra-structure and formation of iron granules in the honeybee (Apis mellifera), Journal Exp. Biology, 180, 1 – 13.
• Jeliazkova I., М. Мarinova, V. Peneva, 2001, Honey bees and their products as bioindicators of environmental pollution. І. Study on mineral content of body of bee-workers received different doses of microelements with their food, Animal science, ХХХVІІІ, 6, 37 – 40.
• Jeliazkova I., М. Маrinova, К. Gurgulova, 2002, Honey bees and their products as bio-indicators of environmental pollution. ІІ. Influence of subfeeding of bee families with microelements contamined sugar solution on content of these elements in bee organism, Animal science, ХХХІХ, 4 – 5, 154 – 157.
• Makarov YU.I., T.L. Cheriatnikova, I.N. Mishin, T.N. Prizova, 1995, An ecological analysis of the environment based on the study of honey bees and of the beehive products, XXXIV-th International Apicultural Congress, Lausanne, Switzerland, Apimondia Publishing House, Bucharest – Romania, 64 – 65.
• Porrini C., P. Medrzycki, L. Bortolotti, A.G. Sabatini, S. Girotti, S. Ghini, F. Grillenzoni, E. Gattavecchia, G. Celli, 2003, Honeybees as bio-indicators of the environmental pollution, XXXVIII-th Apimondia International Apicultural Congress, Ljubljana, Slovenia, Final Programme and Book of Abstracts, 424. Raes H., R. Cornelis, U. Rzeznik, 1992, Distribution, accumulation and deporation of administered lead in adult honeybees, Science Total Environment, 113, 3, 269 – 279.
• Ravetto P., D. Кavalya, V. Colombo, P. Peyla, 1988, A proposal to use bees as an indicator of radioactive pollution, Apiacta, ХХІІІ, 1, 23 – 27.
• Shoven R., 1953, Insect physiology, “Inostrannoy literatury” – Moscow.
• Stark J.A., 2003, The honey-bee (Apis mellifera L.) as monitor of short – and long-term environmental and ecological changes, XXXVIII-th Apimondia International Apicultural Congress, Ljubljana, Slovenia, Final Programme and Book of Abstracts, 284.
• Taranov G.F., 1968, Anatomy and physiology of honey bees, “Kolos” – Moscow.
• Tyshtenko V.P., 1976, Fundamentals of insect physiology. Part І – Physiology of metabolic systems, Leningrad.
• Tyshtenko V.P., 1986, Insect physiology, “Vysshaya shkola” – Moscow.