The Effects of Propolis on Chalkbrood (Ascospheraapis) Disease in Honey Bee Colonies

Yıl 2003, Cilt: 03 Sayı: 4, 37 - 39, 25.11.2003

Nuray Şahinler Şener Kurt Osman Kaftanoğlu

Öz

The effectiveness of Turkish propolis on the pathogen of chalkbrood disease (Ascosphera apis) was studied. The extract was prepared by mixing 1900 ml 70% ethanol and 100 g propolis. Isolated A.apis pathogen were cultured in PDA (Potato Dextrose Agar). Five mm in diameter A.apis culture discs were placed in the petri dishes containing PDA and 50 ppm, 25ppm, 12.5 ppm, 6.25 ppm, 3.125 ppm and 1.56 ppm of 5% propolis extract and incubated at 31 ± 1 °C. The growth of the pathogen was evaluated after the 1 month of incubation period. Propolis extracts was found to be highly effective against to A.apis pathogen in vitro conditions. Keywords: Honeybee, propolis, Ascosphera apis,

Anahtar Kelimeler

Honeybee, propolis, Ascosphera apis

Propolisin Kireç Hastalığı Üzerine Etkileri

Öz

Bu çalışma popolisin Kireç hastalığı etmeni (Ascosphera apis) üzerine etkilerini belirlemek üzere yapılmıştır. Propolis ekstratı 1900 ml % 70 lik etil alkol ve 100 g propolis ile hazırlanmıştır. Đsole edilmiş olana A.apis patojeni PDA (patates Dekstroa Agar)da kültüre alınmıştır. 5 mm2 çapındaki A.apis kültür diskleri içinde PDA ve 50,25, 12.5, 6.25, 3.125 ppm % 5lik proplis ekstratı bulunan petri kutuları içinde 31 ± 1 °C inkübasyona bırakılmıştır. Bir aylık inkübasyon peryodundan sonra patojenin gelişimi değerlendirilmiştir. Propolis ekstratının in vitro koşullarda A.apis patojenine karşı yüksek bir etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Bal arısı, propolis, Ascosphera apis

Kaynakça

• Abdulsalam, K.S. 1995. Bioactivity of propolis extract against certain soil borne fungi.
• Alexandria. Journal of Agricultural Research 40:3, 305-313.
• Bankova, V.S.; Castro, S.L.; Marcucci, M.C.(2000). Propolis: recent advances in chemistry and plant origin. Apidologie 31 P:3-15.
• Bradbear, N.1988. The world distribution of major honeybee diseases and pests. Bee World, 69:15-39.
• Ghisalberti, E.L., (1979). Propolis: A review. Bee World 60(2):P: 59-84.
• Giauffret, A.,Taliercio, Y. 1967. Les mycoses de l’abeille (Apis mellifera) etude de quelques antimycosiques. Bull. Apic. Doc. Scie. Tech. Inf. 10(2): 163-174
• Greenaway, W., Scaysbrook,T., Whatley,F.R., (1990). The Composition and Plant Origins of Propolis: A Report of Work at Oxford. Bee World. 71(3).107-118.
• Kaftanoglu, O., Bicici, M., Yeninar, H., Toker, S., Guler, A.(1992) The effects of formic acid on Varroa jacobsoni and chalkbrood (Ascosphaera apis) disease in honeybee (Apis mellifera L) colonies. Doga-Tr.J.of Veterinary and Animal Science 16(415-425) (In Turkish with Eng. summary).
• Kaftanoglu, O. Yeninar, H., Ozkok, D. (1995) Controlling chalkbrood disease (Ascosphaera apis) in honeybee colonies by using nystatine. Proceedings of Apimondia XXXIVth International Apicultural Congress, pp. 180- 181, Lousanne Switzerland.
• Kaftanoglu, O., Yeninar, H., Kumova U., Ozkok, D. (1997) Epidemiology and control of honeybee (Apis mellifera L.) diseases in Turkey. Final Report. 93 pp. TUBİTAK Project No: VHAG-925, TUBİTAK Publication No: 92-0054, Ankara
• Krell .R. 1996. Value-Added Products From Beekeeping. FAO Agricultural ServicesBulletin No. 124 Food and Agriculture Organization of the United Nations Rome.
• Liu, T.P. 1991. A possible chalkbrood control. Amer. Bee J. 131(8):511
• Puerta, F., Flores, J.M., Ruiz, J.A., Ruz, J.M., and Campano, F. 1997. Fungal diseases of the honeybee (Apis mellifera L.). in: Bee disease diagnosis, eds. M.E. Colin, B.V. Ball, M. Kılanı. Serie B. CIHEAM, Zaragoza.
• Samsinokova, A., Kalolova, S., Haragsim, O. 1977. Effects of some antimycotics and disinfectants on the Ascosphaera apis fungus in vitro. Z. angev. Ent. 84(3): 225-232
• Schmidt, J.O. 1997. Bee Products, Chemical Composition and Application. International Conference on: Bee Product: Properties, Applications and Apitherapy P: 15. Israel.
• Yeninar, H. 1992. The effects of some chemicals on the development of chalkbrood disease (Ascosphaera apis) and the possible control methods. Ms Thesis. 53 pp. C.U. Fen Bilimleri Enst., Adana, Turkey.