Bazı Etanolik Propolis Örneklerinin α-Glukosidaz ve α-Amilaz İnhibisyonu

Yıl 2021, Cilt: 21 Sayı: 1, 1 - 7, 12.05.2021

Nimet Baltaş

https://doi.org/10.31467/uluaricilik.877301

Cited By: 9

Öz

Metabolik bozuklukların iyileştirilmesinde sentetik olmayan takviyelerin olası kullanımı nedeniyle, doğal bir ürün olan propolis büyük ilgi görmektedir. Bu iddiayı desteklemek için mevcut çalışma planlandı ve ortaya kondu. Bu çalışmada, Türkiye'nin farklı bölgelerinden elde edilen altı propolis ekstratının tedavi amacıyla kullanılabilmesi adına faydalı özelliklerini ortaya koymak için bazı enzimlere karşı inhibisyon etkileri ve antioksidan seviyeleri araştırıldı. α-glukozidaz IC50 sonuçları 0,208-0,426 mg/mL ve α-amilaz IC50 sonuçları 0,487-0,938 mg / mL aralığında bulundu. Ayrıca, inhibisyon etki derecelerini desteklemek için antioksidan aktivite sonuçları da verildi. Toplam fenolik (TPC) ve antioksidan verilerine göre, S4 en verimli örnek olarak kaydedildi. Propolisin biyolojik etkilerini araştırmak için ileride yapılacak yeni çalışmalara ihtiyaç vardır, ancak sonuç olarak, propolisin beslenme ve klinik potansiyeli olarak dikkate değer bir kaynak olabileceğini göstermektedir.

Anahtar Kelimeler

α-glukozidaz, α-amilaz, enzim inhibisyonu, antioksidan, propolis

α-GLUCOSIDASE AND α-AMYLASE INHIBITION OF SOME ETHANOLIC PROPOLIS SAMPLES

Öz

Propolis is a natural product, and it is of a great interest due to the possible uses of non-synthetic supplements in improving metabolic disorders. To support this claim, the current study was designed and presented. In this study, six propolis extracts obtained from different location of Turkey were investigated to prove the beneficial therapeutic properties such as inhibition potent against some enzymes and levels of antioxidant. IC50 results of α-glucosidase (0.208-0.426 mg/mL) and α-amylase (0.487-0.938 mg/mL) were found the variable range. Moreover, antioxidant results of them were given to support the inhibition degrees. According to the total phenolic (TPC) and antioxidant data, S4 was noted as the most efficient sample. Future studies are needed to investigate the biological effects of propolis, but the ultimate evaluating showed that it could be a significant source thanks to its nutritional and clinical potential.

Anahtar Kelimeler

α-glucosidase, α-amylase, enzyme inhibition, antioxidant, propolis

Kaynakça

• Baltas, N., Yildiz, O., Kolayli, S. (2016). Inhibition properties of propolis extracts to some clinically important enzymes. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(sup1), 52–55. https://doi.org/10.3109/14756366.2016.1167049.
• Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5.
• Izuta, H., Narahara, Y., Shimazawa, M., Mishima, S., Kondo, S., Hara, H. (2009). 1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Activity of Bee Products and Their Constituents Determined by ESR. Biological & Pharmaceutical Bulletin, 32(12), 1947–1951. https://doi.org/10.1248/bpb.32.1947.
• Kedare, SB., Singh, RP. (2011). Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48(4), 412–422. https://doi.org/10.1007/s13197-011-0251-1.
• Lee, KJ., Oh, YC., Cho, WK., Ma, JY. (2015). Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay. Evidence-Based Complementary and Alternative Medicine : ECAM, 2015, 165457. https://doi.org/10.1155/2015/165457.
• Mekonnen Abebe, S., Alemu Balcha, S. (2012). The Effect of Supervised Progressive Resistance Training (PRT) on Glycemic Control and Cardio Vascular Disease (CVD) Risk Markers in Type 2 Diabetes Patients, North West Ethiopian. Journal of Diabetes & Metabolism, 3(172), 1–5. https://doi.org/10.4172/2155-6156.1000172.
• Miguel, MG., Nunes, S., Dandlen, SA., Cavaco, AM., Antunes, M. D. (2014). Phenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal. Food Science and Technology, 34(1), 16–23.
• Özil, M., Parlak, C., Baltaş, N. (2018). A simple and efficient synthesis of benzimidazoles containing piperazine or morpholine skeleton at C-6 position as glucosidase inhibitors with antioxidant activity. Bioorganic Chemistry, 76, 468–477. https://doi.org/10.1016/J.BIOORG.2017.12.019.
• Popovaa, M., Lyoussib, B., Aazzac, S., Antunesc, D., Bankovaa, V., Miguel G. (2015). Antioxidant and α-Glucosidase Inhibitory Properties and Chemical Profiles of Moroccan Propolis. Natural Product Communications, 10 (11),1961-1964. https://doi.org/10.1177/1934578X1501001139.
• Ramnath, S., Venkataramegowda, S. (2016). Antioxidant Activity of Indian Propolis-An In Vitro Evaluation. International Journal of Pharmacology, Phytochemistry and Ethnomedicine, 5, 79–85. https://doi.org/10.18052/www.scipress.com/IJPPE.5.79.
• Ramnath, S., & Venkataramegowda, S. (2017). Anti-Inflammatory And Anti-Diabetic Activity of Indian Propolis. European Journal of Pharmaceutıcal And Medical Research, 4(1), 311–316.
• Rasouli, H., Hosseini-Ghazvini, SM. B., Adibi, H., Khodarahmi, R. (2017). Differential α-amylase/α-glucosidase inhibitory activities of plant-derived phenolic compounds: a virtual screening perspective for the treatment of obesity and diabetes. Food & Function, 8(5), 1942–1954. https://doi.org/10.1039/C7FO00220C.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26(9–10), 1231–1237.
• Salah, NM., A Souleman, AM., Shaker, KH., El Hawary, S., El-Shahid, ZA., Abd El-Hady, FK. (2017). Acetylcholinesterase, Alpha-Glucosidase and Tyrosinase Inhibitors from Egyptian Propolis. Available Online on Www.Ijppr.Com International Journal of Pharmacognosy and Phytochemical Research, 9(4), 528–536. https://doi.org/10.25258/phyto.v9i2.8126.
• Singleton, VL., Rossi, JA. (1965). Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158.
• Socha, R., Gałkowska, D., Bugaj, M., Juszczak, L. (2015). Phenolic composition and antioxidant activity of propolis from various regions of Poland. Natural Product Research, 29(5), 416–422. https://doi.org/10.1080/14786419.2014.949705.
• Souza, EA., Zaluski, R., Veiga, N., Orsi, RO. (2016). Effects of seasonal variations and collection methods on the mineral composition of propolis from Apis mellifera Linnaeus Beehives. Brazilian Journal of Biology, 76(2), 396–401. https://doi.org/10.1590/1519-6984.16714.
• Tan, C., Wang, Q., Luo, C., Chen, S., Li, Q., Li, P. (2013). Yeast α-glucosidase inhibitory phenolic compounds isolated from Gynura medica leaf. International Journal of Molecular Sciences, 14(2), 2551–2558. https://doi.org/10.3390/ijms14022551
• Telagari, M., Hullatti, K. (2015). In-vitro α-amylase and α-glucosidase inhibitory activity of Adiantum caudatum Linn. and Celosia argentea Linn. extracts and fractions. Indian Journal of Pharmacology, 47(4), 425–429. https://doi.org/10.4103/0253-7613.161270
• Unnikrishnan, PS., Suthindhiran, K., Jayasri, M. A. (2015). Alpha-amylase Inhibition and Antioxidant Activity of Marine Green Algae and its Possible Role in Diabetes Management. Pharmacognosy Magazine, 11(Suppl 4), 511-515. https://doi.org/10.4103/0973-1296.172954
• Vongsak, B., Kongkiatpaiboon, S., Jaisamut, S., Machana, S., Pattarapanich, C., Vongsak, B., Pattarapanich, C. (2015). In vitro alpha glucosidase inhibition and free-radical scavenging activity of propolis from Thai stingless bees in mangosteen orchard. Revista Brasileira de Farmacognosia, 25(5), 445–450. https://doi.org/10.1016/j.bjp.2015.07.004.