Serum Leptin Levels During the Dry Period and Various Lactation Stages in Holstein Dairy Cows

Öz

In this study we investigated the effects of dry period and various stages of lactation on serum leptin, glucose, total cholesterol and β hydroxybutyrate levels in Holstein dairy cows. Fourthy, four years old Holstein dairy cows in their second lactation were used as material. Ten of them were in dry period and the rest were in different stages of lactation periods (10 cows for each stages). Serum concentrations of leptin, glucose, total cholesterol and β hydroxybutyrate were compared between the dry period, and first, second and third stages of lactation period. Correlation between leptin and other parameters were analyzed. While no significant differences were observed in β hydroxybutyrate levels, significant differences were determined for leptin, glucose and total cholesterol levels between dry period and first, second, and third stages of lactation period (p<0.05, p<0.001, and p<0.001, respectively). Statistically significant positive correlations were observed between leptin and glucose concentrations.(r =0,401, p<0.01 ).

Anahtar Kelimeler

• Leptin
• Dairy Cows
• Biochemical Parameters
• Lactation Period

Kuru Dönem ve Laktasyonun Çeşitli Evrelerindeki Holstein Irkı Süt İneklerinde Serum Leptin Düzeyleri

Öz

Bu çalışmada Holstein ırkı süt ineklerinde kuru dönem ve laktasyonun çeşitli evrelerinin serum leptin, glukoz, total kolesterol ve β hidroksi bütirat düzeyleri üzerine etkisi araştırıldı. Materyal olarak 40 adet, ikinci laktasyon döneminde olan, 4 yaşında holstein ırkı süt ineği kullanıldı. İneklerin 10 tanesi kuru dönemde, geri kalanlar ise laktasyon periyodunun çeşitli evrelerindeydiler (her evrede 10 inek). Kuru dönem ve laktasyonun I., II., ve III. evreleri arasında serum leptin, glukoz, total kolesterol ve β hidroksi bütirat konsantrasyonları karşılaştırıldı. Leptin ve diğer parametreler arasındaki korelasyonlar analiz edildi. Kuru dönem ve laktasyon periyodunun I., II., ve III. evreleri arasında β hidroksi bütirat düzeylerinde önemli bir farklılık gözlenmezken, leptin, glukoz ve total kolesterol düzeylerinde önemli farklılıklar ortaya koyuldu (p < 0.05, p < 0.001, and p < 0.001, sırasıyla). Leptin ve glukoz konsantrasyonları arasında istatistiki olarak pozitif bir korelasyon olduğu ortaya koyuldu (r =0,401, p < 0.01 )

Anahtar Kelimeler

• Leptin
• Süt İneği
• Biyokimyasal Parametreler
• Laktasyon Periyodu

Kaynakça

1. Accorsi, P.A., Govoni, N., Gaiani, R., Pezzi, C., Seren, E., Tamanini, C., 2005. Leptin, GH, PRL, Insulin and Metabolic Parameters Throughout the Dry Period and Lactation in Dairy Cows. Reprod Dom Anim., 40, 217–223.
2. Andersson, L., Emanuelson, U., 1985. An epide- miological study of hyperketonaemia in Swedish dairy cows; determinants and the relation to fer- tility. Prev Vet Med., 3:449–462.
3. Andersson, L., 1988. Subclinical ketosis in dairy cows. Metabolic diseases of ruminant livestock. Vet Clin North Am Food Anim Pract., 4, 233– 251.
4. Barzilai, N., Wang, J., Massilon, D., Vuguin, P., Hawkins, M., Rossetti, L., 1998. Leptin selective- ly decreases visceral adiposity and enhances in- sulin action. J Clin Invest., 100, 3105–3110.
5. Block, S.S., Butler, W.R., Ehrhardt, R.A., Bell, A.W., Amburgh, M.E., Boisclair, Y.R., 2001. Decreased concentration of plasma leptin in peri- parturient dairy cows is caused by negative ener- gy balance. J Endocrin., 171, 339–348.
6. Block, S.S., Rhoads, R.P., Bauman, D.E., Ehrhardt, R.A., McGuire, M.A., Crooker, B.A., Griinari, J.M., Mackle, T.R., Weber, W.J., Van, Amburgh, M.E., Boisclair, Y.R., 2003. Demon- stration of a role for insulin in the regulation of leptin in lactating dairy cows. J Dairy Sci 86, 3508–3515.
7. Bruss, M.L., 1997. Lipids and ketones. In: Kaneko JJ, Harvey JW, Bruss ML (eds) Clinical biochemistry of domestic animals, 5th edn. Aca- demic, London, pp 83–113.
8. Chelikani, P.K., Keisler, D.H., Kennelly, J.J., 2003. Response of Plasma Leptin Concentration to Jugular Infusion of Glucose or Lipid Is De- pendent on the Stage of Lactation of Holstein Cows. J Nutr., 133, 4163-4171

9. Dohoo, I.R., Martin, S.W., 1984. Subclinical ketosis: prevalence and associations with produc- tion and disease. Can J Comp Med., 48:1–5.
10. Lei, Z.M., Ye M.X., Fu W.G., Chen Y., Fang C., Li J., 2008. Levels of serum leptin, cholecysto- kinin,plasma lipid and lipoprotein differ between patients with gallstone or/and those with hepato- lithiasis. Hepatobiliary Pancreat Dis Int.，7, 65- 69.
11. Liefers, S.C., Veerkamp, R.F., Pas, M.F.W., Delavaud, C., Chilliard, Y., Lende V.T., 2003. Leptin concentrations in relation to energy bal- ance, milk yeld, intake, live weight, and estrus in dairy cows. J Dairy Sci., 86, 799–807.
12. Mueller, G., Ertl, J., Gerl, M., Preibisch, G., 1997. Leptin impairs metabolic actions of insulin in isolated rat adipocytes. Science., 272, 10585– 10593.
13. Parola, R., Macchi, E., Fracchia, D., Sabbioni, A., Avanzi, D., Motta, M., Accornero, P., Barata, M., 2007. Comparison between plasma and milk levels of leptin during pregnancy and lactation in cow, a relationship with b-lactoglobulin. J Anim Physiol Anim Nutr., 91, 240–246.
14. Reist, M., Erdin, D., Euw, D.V., Tschuemperlin, K., Leuenberger, H., Delavaud, C., Chilliard, Y., Hammon, H.M., Kuenzi, N., Blum, J.W., 2003. Concentrate Feeding Strategy in Lactating Dairy Cows: Metabolic and Endocrine Changes with Emphasis on Leptin. J Dairy Sci., 86:1690-1706.
15. Sivitz, W.I., Walsh, S.A., Morgan, D.A., Thom- as, M.J., Haynes, W.G., 1997. Effects of leptin on insulin sensitivity in normal rats. Endoc., 138, 3395-3401.
16. Whitaker, D.A., Smith, E.J., Rosa, G.O., 1993. Some effects of nutrition and management on the fertility of dairy cattle. Vet Rec., 133:61–64.
17. Zhou, Y.T., Shimabukuro, M., Koyama, K., Lee, Y., Wang, M.Y., Trieu, F., Newgard, C.B., Un- ger, R.H., 1997. Induction by leptin of uncou- pling protein-2 and enzymes of fatty acid oxida- tion. Proc Natl Acad Sci USA., 94, 6386–6390.