Effect of oleuropein on element distributions in liver of diabetic rats

Abstract

Keywords

• diabetes,rat,element,, oleuropein

Effect of oleuropein on element distributions in liver of diabetic rats

Öz

There is accumulating evidence demonstrating that the metabolism of many trace elements is modified in diabetes mellitus. In addition, essential elements and minerals are key to nutrition and sound health. Oleuropein, a major phenolic compound in olives, is known to reduce the blood glucose levels in alloxaninduced diabetic rats and rabbits. The purpose of this study was to compare the levels of essential trace elements, selenium (Se), manganese (Mn), copper (Cu), chromium (Cr) and zinc (Zn) in Strepsttozotocin (STZ)-induced diabetic rats and to evaluate the effects of oleuropein on trace elements levels. Animals were apportioned into 4 groups of 8 rats each. The control group was fed with standard rat provender and got no added treatment. In the oleuropein group, 20 mg/kg dosages of oleuropein were given to normal animals intraperitoneally (i.p) for 28 days. In the diabetic group, STZ was injected to rats at a single dose of 50 mg/kg i.p. The last group, 20 mg/kg dosages of oleuropein were given to diabetic animals i.p for 28 days. In this study, trace elements levels were evaluated by using ICP-MS and MDA, SOD CAT levels were evaluated by using spectrophotometers methods.

Trace elements levels were significantly (p <0,05) decreased in diabetic rats liver but oleuropein was significantly (p<0,05) increased trace element levels in this group.

In the diabetic group, serum blood glucose levels were significantly (p <0,05)  increased and STZ increased total oxidant status (TOS), malondialdehyde (MDA) in the liver, whereas it decreased superoxide dismutase (SOD) and catalase (CAT) and total antioxidant capacity (TAS) in diabetic rats liver.

As a consequence, oleuropein treatment shows an antioxidant and in diabetes by reducing oxidative stress and it was increased trace element levels.

Anahtar Kelimeler

• Rat,diabetes,element,oleuropein

Kaynakça

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