TARİHİ YAPILARDAKİ ÇATLAKLARIN Bacillus amyloliquefaciens U17 KULLANILARAK BİYOMİNERALİZASYON YÖNTEMİYLE ONARIMI

Öz

Tarihi yapılar, yalnızca günlük yaşam çevremizin bir unsurunu oluşturmamakta; aynı zamanda geçmişin günümüze ulaşan canlı temsilcileri olarak toplumların ve toplumu oluşturan kültürel toplulukların varlığını, kimliğini ve sürekliliğini belgelemektedir. Bu nedenle kültürel ve mimari mirasın korunması ile gelecek kuşaklara aktarılması, bütün insanlığın ortak sorumluluğu niteliğindedir. Yapılan bu çalışmada Aksaray ve çevre illerdeki tarihi yapılarda tercih edilen volkanik tüf taşı kullanılarak biyomineralizasyon yöntemiyle, taş üzerinde oluşturulan çatlakların kapatılması incelenmiştir. Denizli (Türkiye) kireçli toprağından izole edilen, özellikle beton ve yapı malzemelerinin biyolojik yöntemlerle onarımı/iyileştirilmesinde kullanılan üreolitik, kalsiyum karbonat çökeltebilen özel bir bakteri suşu ve yerel izolat olan Bacillus amyloliquefaciens U17 ve literatür incelemelerinde kontrol olarak kullanılan yüksek üreaz aktivitesine sahip, çubuk formunda, gram-pozitif, aerobik ve uç spor oluşturan Sporosarcina pasteurii ATCC 6453 karşılaştırılarak çatlakları kapatma potansiyeli incelenmiştir. Elde edilen veriler, mikroskop görüntüleri ile SEM ve EDAX analizleri doğrultusunda incelenmiş; yerel izolat Bacillus amyloliquefaciens U17’nin, volkanik tüf taşı ile uyumlu şekilde, kontrol suşuna kıyasla belirgin derecede daha yüksek çatlak kapatma verimliliği sergilediğini ortaya koymuştur.

Anahtar Kelimeler

• Bakteriyel Biyomineralizasyon
• Kalsiyum Karbonat Çökelimi
• Çatlak Onarımı
• Mikro-Çatlaklar
• Kendi Kendine İyileşme
• Sürdürülebilirlik

Destekleyen Kurum

Aksaray Üniversitesi Bilimsel Araştırma Projesi Koordinatörlüğü

Proje Numarası

2023-038

Etik Beyan

Çalışmanın tüm süreçlerinin araştırma ve yayın etiğine uygun olduğunu, etik kurallara ve bilimsel atıf gösterme ilkelerine uyduğumu beyan ederim.

Teşekkür

Bu çalışma Aksaray Üniversitesi Bilimsel Araştırma Projesi Koordinatörlüğü tarafından 2023-038 numaralı proje ile desteklenmiştir.

Repair of Cracks in Historical Structures by the Biomineralization Method Using Bacillus amyloliquefaciens U17

Öz

Historic buildings are not merely an element of our everyday environment; they also serve as living representatives of the past that have survived to the present day, documenting the existence, identity and continuity of societies and the cultural communities that comprise them. For this reason, the preservation and transmission of cultural and architectural heritage to future generations is a shared responsibility of all humanity. This study investigated the closure of cracks formed on stone using the biomineralisation method, employing volcanic tuff stone—a material commonly used in historic buildings in Aksaray and neighbouring provinces. A ureolytic, calcium carbonate-precipitating bacterial strain isolated from calcareous soil in Denizli (Turkey), specifically used for the biological repair/rehabilitation of concrete and building materials, and the local isolate Bacillus amyloliquefaciens U17, were compared with Sporosarcina pasteurii ATCC 6453—a rod-shaped, gram-positive, aerobic bacterium that forms spores and is used as a control in literature reviews—to investigate their potential for sealing cracks. rod-shaped, Gram-positive, aerobic and endospore-forming strain Sporosarcina pasteurii ATCC 6453, which is used as a control in literature reviews, to investigate their potential for crack sealing. The data obtained were analysed in conjunction with microscopic images, SEM and EDAX analyses; the results demonstrated that the local isolate Bacillus amyloliquefaciens U17 exhibited significantly higher crack-sealing efficiency compared to the control strain, demonstrating compatibility with volcanic tuff.

Anahtar Kelimeler

• Bacterial Biomineralization
• Calcium Carbonate Precipitation
• Crack Repair
• Micro Cracks
• Self Healing
• Sustainability

Destekleyen Kurum

Aksaray University Scientific Research Project Coordination Office

Proje Numarası

2023-038

Etik Beyan

I declare that all processes of this study comply with research and publication ethics, and that I have adhered to ethical rules and principles of scientific citation.

Teşekkür

This study was supported by the Aksaray University Scientific Research Project Coordination Office with project number 2023-038.

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