Investigation of Mechanisms Involved in the Regulation of Basal Vascular Tone in a Rat Thoracic Aortic Model

Öz

In this study, it was aimed to determine the effect of endothelial nitric oxide synthase (eNOS)/nitric oxide (NO), cyclooxygenase (COX), AMP-activated protein kinase (AMPK), mitogen-activated protein kinase (MAPK), and apelin receptor (APJ) signal-transducing pathways and potassium channels on vascular tone. 4 mm vascular rings obtained from the thoracic aorta of Wistar Albino male rats were placed in the isolated organ bath system. Vascular tension was adjusted to 1 gram. To determine the effects of signal-transducing pathways and potassium channels on basal vessel tone, inhibitors were administered after a 1-hour equilibration period. The tension values in the periods before and after the inhibitor application were recorded. Nω-Nitro-L-arginine methyl ester and tetraethylammonium administrations caused a statistically significant increase in basal vascular tension values (in order: p < 0.001; p < 0.05). Indomethacin and dorsomorphin administrations caused a statistically significant decrease in basal vascular tension values (p < 0.05). F13A and U0126 administrations didn’t cause a statistically significant change in basal vascular tension values. The data of this study show that eNOS/NO, COX, and AMPK signal-transducing pathways and potassium channels have significant effects on the regulation of basal vascular tone. On the other hand, it is thought that MAPK and APJ signaling pathways aren’t important factors in the regulation of basal vascular tone in the rat thoracic aorta.

Anahtar Kelimeler

• AMPK
• COX
• NO
• potassium channel
• vascular tone

Bazal Vasküler Tonusun Düzenlenmesinde Rol Alan Mekanizmaların Sıçan Torasik Aort Modelinde İncelenmesi

Öz

Bu çalışmada, endotelyal nitrik oksit sentaz (eNOS)/nitrik oksit (NO), siklooksijenaz (COX), AMP ile aktive olan protein kinaz (AMPK), mitojen ile aktive edilen protein kinaz (MAPK) ve apelin reseptörü (APJ) sinyal ileti yolakları ile potasyum kanallarının vasküler tonus üzerindeki etkisinin belirlenmesi amaçlandı. Wistar Albino erkek sıçanların torasik aortlarından elde edilen 4 mm’lik damar halkaları izole organ banyosu sistemine yerleştirildi. Damar gerimi 1 gram olarak ayarlandı. Sinyal ileti yolaklarının ve potasyum kanallarının bazal damar tonusu üzerindeki etkilerini belirlemek için 1 saatlik dengelenme periyodunu takiben inhibitör madde uygulamaları yapıldı. İnhibitör madde uygulamalarından önceki ve sonraki periyodlardaki gerim değerleri kaydedildi. Nω-Nitro-L-arginin metil ester ve tetraetilamonyum uygulamaları bazal damar gerim değerlerinde istatistiksel olarak anlamlı düzeyde artışa neden oldu (sırasıyla: p < 0,001; p < 0,05). İndometazin ve dorsomorfin uygulamaları bazal damar gerim değerlerinde istatistiksel olarak anlamlı düzeyde azalmaya neden oldu (p < 0,05). F13A ve U0126 uygulamaları bazal damar gerim değerlerinde istatistiksel olarak anlamlı düzeyde bir değişikliğe neden olmadı. Bu çalışmanın verileri eNOS/NO, COX ve AMPK sinyal ileti yolakları ile potasyum kanallarının bazal vasküler tonus regülasyonunda önemli birer etken olduğunu göstermektedir. Buna karşın MAPK ve APJ sinyal ileti yolaklarının sıçan torasik aortundaki bazal vasküler tonus düzenlenmesinde önemli birer faktör olmadığı düşünülmektedir.

Anahtar Kelimeler

• AMPK
• COX
• NO
• potasyum kanalı
• vasküler tonus

Teşekkür

Bu çalışma Bursa Uludağ Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı’nın imkanları ile gerçekleştirilmiştir.

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