Effects of Uridine and Uridine Nucleotides on Proliferation and Migration of L929 Murine Fibroblast Cell Line

Öz

The present study aimed to investigate the effects of uridine and the uridine nucleotides uridine-5’-monophosphate (UMP), uridine-5’-diphosphate (UDP) and uridine-5’-triphosphate (UTP) at different concentrations (1, 10 and 100 μM) on cell viability and migration capacity using the L929 murine fibroblast cell line. To assess cytotoxicity and cellular proliferation, the MTT [(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)] assay was performed at 24, 48 and 72 hours of incubation, providing a quantitative evaluation of metabolic activity and cell survival. Additionally, the scratch assay was employed and followed up to 96 hours to analyze fibroblast migration, offering insights into the role of these pyrimidines in wound healing and tissue repair. The MTT assay revealed that the highest concentration (100 μM) of UMP or UDP significantly enhanced proliferation of cells at 72 hours while uridine at 10 and 100 μM and UTP at all concentrations tested provided the same effect. In scratch assay UMP or UDP did not cause any significant cell migration while uridine and UTP, at all concentrations, significantly enhanced migration of fibroblast cells at 96 hours. The results demonstrated distinct effects of uridine and its nucleotides on cell viability and migration, with significant benefit in terms of wound healing provided by uridine and UTP, highlighting their potential biological significance and therapeutic implications in regenerative medicine and tissue engineering.

Anahtar Kelimeler

• uridine
• L929 murine fibroblast cells
• MTT
• proliferation
• migration
• wound healing

Uridin ve Uridin Nükleotidlerinin L929 Fare Fibroblastik Hücrelerinin Proliferasyonu ve Göçü Üzerindeki Etkileri

Öz

Bu çalışmanın amacı, üridin ve üridin nükleotidleri üridin-5’-monofosfat (UMP), üridin-5’-difosfat (UDP) ve üridin-5’-trifosfat (UTP)’ın farklı konsantrasyonlarda (1, 10 ve 100 μM) uygulanmasının L929 kemirgen fibroblast hücre kültüründe hücre canlılığı ve göç etme kapasitesine etkilerini incelemektir. Sitotoksisite ve hücresel proliferasyonu değerlendirmek için 24, 48 ve 72 saatlik inkübasyonların sonunda metabolik aktivite ve hücre canlılığı konusunda kantitatif değerlendirme yapılmasını sağlayan MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür] testi uygulandı. Ayrıca bu pirimidin bileşiklerinin yara iyileşmesi ve doku tamiri konusundaki etkilerini incelemek üzere çizik testi yapılarak fibroblast göçü 96 saate kadar takip edildi. MTT testi ile 72 saat inkübasyon sonunda UMP veya UDP’nin en yüksek (100 μM) konsantrasyonda hücre proliferasyonunu artırırken üridin’in 10 ve 100 μM konsantrasyonlarda ve UTP’nin denenen tüm konsantrasyonlarında aynı etkiyi gösterdiği tespit edildi. Çizik testinde UMP veya UDP anlamlı hücre göçüne neden olmazken 96 saat inkübasyon sonrası üridin ve UTP denenen tüm konsantrasyonlarında hücre göçünü anlamlı olarak artırdı. Bu sonuçlar üridin ve üridin nükleotidlerinin hücre proliferasyonu ve göçü üzerine farklı etkileri olduğunu ve yara iyileşmesi bakımından üridin ve UTP’nin anlamlı faydaları olabileceğini göstermekle birlikte rejeneratif tıp ve doku mühendisliği alanlarındaki potansiyel biyolojik önemlerine ve terapötik kullanımlarına işaret etmektedir.

Anahtar Kelimeler

• üridin
• L929 kemirgen fibroblast hücreleri
• MTT
• proliferasyon
• migrasyon
• yara iyileşmesi

Kaynakça

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