Çinko Oksit Katkılı Bakteriyel Selüloz Nanokompozitlerinin Hücre Canlılığı ve Proliferasyonu Üzerine Etkilerinin Araştırılması

Öz

Bu çalışmada, Komagataeibacter rhaeticus suşu kullanılarak üretilen bakteriyel selüloz (BS) membranlarına farklı konsantrasyonlarda çinko oksit (ZnO) nanoparçacıkları eklenerek nanokompozit sentezlenmesi ve bu materyallerin ZnO salınım kinetiği ile L929 fibroblast hücreleri üzerindeki sitotoksisite ve proliferasyon etkilerinin değerlendirilmesi amaçlanmıştır. BS membranları Hestrin-Schramm besiyerinde statik kültür koşullarında üretildikten sonra, daldırma yöntemi kullanılarak %1 ile %3 arasında değişen ZnO konsantrasyonlarında BS-ZnO nanokompozitleri hazırlanmıştır. ZnO salınımı 7 gün boyunca PBS ortamında UV-VIS spektrofotometre ile izlenmiş; veriler Higuchi ve Korsmeyer-Peppas modelleri ile analiz edilmiştir. Biyouyumluluk testleri için L929 fibroblast hücrelerinde MTT (24, 48, 72. saat) ve hücre göçü için Scratch assay testleri uygulanmıştır. Salınım testleri, ZnO konsantrasyonu arttıkça difüzyon hızının azaldığını ve %1 ZnO içeren örneklerin Korsmeyer-Peppas modeline (n=0,7; Non-Fickian difüzyon) en iyi uyumu gösterdiğini ortaya koymuştur. MTT sonuçlarına göre, %0,1 ve %0,5 ZnO içeren kompozitlerin toksisite göstermediği ve 48-72. saatlerde proliferasyonu anlamlı düzeyde artırdığı saptanmıştır (p<0,05). Scratch assay analizleri, özellikle %0,1 ve %0,5 ZnO gruplarının 3. ve 4. günlerde kontrol grubuna kıyasla daha hızlı yara kapanması sağladığını doğrulamıştır. Çalışma sonuçları, düşük konsantrasyonlarda ZnO ile modifiye edilen BS membranlarının biyouyumlu olduğunu ve hücre çoğalmasını desteklediğini göstermektedir. Bu nanokompozitlerin gelişmiş yara örtüsü uygulamaları için potansiyel bir aday olduğu öngörülmektedir.

Anahtar Kelimeler

• bakteriyel selüloz
• sitotoksisite
• nanokompozit
• çinko oksit
• biyouyumluluk

Destekleyen Kurum

Bursa Uludağ Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

THIZ-2023-1520

Investigation of the Effects of Zinc Oxide-Modified Bacterial Cellulose Nanocomposites on Cell Viability and Proliferation

Öz

The aim of this study was to synthesize nanocomposites by incorporating zinc oxide (ZnO) nanoparticles into bacterial cellulose (BC) membranes produced by Komagataeibacter rhaeticus and to evaluate the ZnO release kinetics, cytotoxicity, and proliferation effects on L929 fibroblast cells. BC membranes were produced under static culture conditions in Hestrin-Schramm medium and BC-ZnO nanocomposites were prepared via the immersion method at concentrations ranging from 1% to 3% ZnO. ZnO release was monitored for 7 days in PBS using UV-VIS spectrophotometry and analyzed using Higuchi and Korsmeyer-Peppas models. Biocompatibility was assessed using the MTT assay (24, 48, 72 h) and Scratch assay for cell migration on L929 fibroblasts. Release studies indicated that increasing ZnO concentration decreased the diffusion rate, with 1% ZnO samples fitting the Korsmeyer-Peppas model (n=0.7; Non-Fickian diffusion) most accurately. MTT results showed that ZnO concentrations lower than 1% were non-toxic and significantly enhanced proliferation at 48-72 h (p<0.05). Scratch assay analysis confirmed that 0.5% and 0.1% ZnO groups achieved faster wound closure at 72 and 96 hours compared to control groups (p<0.05). The findings suggest that BC membranes modified with low concentrations of ZnO are biocompatible and support cell proliferation. These nanocomposites are promising candidates for advanced wound dressing applications.

Anahtar Kelimeler

• bacterial cellulose
• cytotoxicity
• nanocomposite
• zinc oxide
• biocompatibility

Destekleyen Kurum

Scientific Research Projects Coordination Office of Bursa Uludag University

Proje Numarası

THIZ-2023-1520

Kaynakça

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