Serum GRP-78 Düzeyleri Tedaviden 3 Ay Sonrasında Halen Yüksek Seyretmektedir: Bir Kohort Çalışması

Yıl 2021, , 17 - 22, 01.04.2021

Ramazan Sabırlı , Aylin Köseler , Tarık Gören Aykut Kemancı , Neslihan Türkçüer İbrahim Türkçüer , Özgür Kurt

https://doi.org/10.32708/uutfd.858821

Öz

GRP-78 proteininin bat koronavirüs, Mers-Cov, ebola virüs, deng virüsü, japon ensefalit virüsü, influenza virüs ve zika virüs gibi birçok virüsün hücreye girişinde rol oynadığı bilinmektedir. Bu çalışmada COVID-19 enfeksiyonu geçirmiş ve tedavi almış ve tamamen iyileşmiş olan hastalarda tedavi başlangıcından üç ay sonrasındaki Glucose Regulated Protein-78 (GRP-78) düzeylerini incelemeyi amaçladık. Daha öncesinde Sabırlı ve ark. tarafından yapılan çalışma grubunda yer alan, COVID-19 hastalığı tanısı almış ve hastalığı geçirip tamamen iyileşmiş olan 20 hasta prospektif kohorta dahil edildi. Hastaların acil servise ilk tanıda başvurusu ve 3 ay sonra kontrole çağrıldığında alınan kanlardan enzyme-linked immunosorbent assay (ELISA) metodu ile GRP-78 düzeyi çalışıldı. Acil servise ilk başvuruda alınan kanda serum GRP-78 düzeyi 1393,31 ± 306,33 pg/ml; tedavi başlangıcından 90 gün sonra bakılan serum GRP-78 düzeyi ise 1451,73 ± 336,65 pg/ml olarak saptandı. İlk başvuru ve 3 ay sonraki kontrolde ölçülen GRP-78 düzeyleri açısından istatistiksel olarak anlamlı farklılık saptanmadı (p=0,451). Sonuç olarak bu çalışmada COVID-19 infeksiyonunda tedavi başlangıcından 3 ay sonrasında dahi yüksek seyrettiğini ortaya koyduk. GRP-78 düzeyinin yüksek kalmasının kişinin Sars-CoV-2 virüsüne karşı immunitesi konusunda fikir verdirici olabilir fakat bu hususun gerek hücre kültürü çalışması ve gerekse daha uzun süreli kohort çalışması yapılarak incelenmesine ihtiyaç vardır.

Anahtar Kelimeler

Endoplazmik Retikulum Stresi, GRP-78, Covid19 enfeksiyonu, pnömoni.

Serum GRP-78 Levels Still Remain High 3 Months Later After the Treatment: A Cohort Study

Öz

Glucose Regulated Protein (GRP-78) plays a role in the intrusion of many viruses such as bat coronavirus, MERS-CoV, ebola virus, dengue virus, Japanese encephalitis virus, influenza virus, and Zika virus. This study, however, aims to examine the GRP-78 levels in patients who were infected with COVID-19, treated and recovered completely three months after the initiation of treatment. A total of 20 patients who were diagnosed with the COVID-19 disease and fully recovered, and who had participated in a previous study conducted by Sabırlı et al., were included in this prospective cohort study. Using the enzyme-linked immunosorbent assay (ELISA) method, the GRP-78 levels were examined in the blood samples. The mean serum GRP-78 level was found to be 1393.31 ± 306.33 pg / ml in the blood samples drawn from the patients when they were first admitted to the emergency department while the mean serum GRP-78 level measured 90 days after the initiation of treatment was 1451.73 ± 336.65 pg / ml. No statistically significant differences were found between the GRP-78 levels measured during the first admission and the follow-up control 3 months later (p = 0.451). In conclusion, this study revealed that the GRP-78 levels remained high in patients with COVID-19 infections even after 3 months following the initiation of treatment. This high GRP-78 level may provide insight into the immunity of the person against the Sars-CoV-2 virus; however, this issue should be further examined both in a cell culture study and in a longer-term cohort study.

Anahtar Kelimeler

Endoplasmic Reticulum Stress, GRP-78, Covid19 infection, pneumonia

Kaynakça

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