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Investigation of Anticytotoxic and Antigenotoxic Effect of Rosmarinic Acid Against Cisplatın In A549 and Beas-2B Cell Lines

Yıl 2019, Cilt: 45 Sayı: 3, 263 - 270, 01.12.2019
https://doi.org/10.32708/uutfd.613912

Öz

Rosmarinic Acid (RA) is an important phenolic compound found in many plant species. The aim of this study was to determine the efficacy of RA against  Cisplatin-induced cytotoxic, genotoxic effects and oxidative stress in A549 (Human Lung Cancer Cell Line) and Beas-2B (Human Healthy Bronchial Epithelial Cell Line) cells. In this way, it was aimed to determine the possible effects of RA-containing herbal products that can be used by patients, on Cisplatin treatment during Cisplatin chemotherapy. For this purpose, XTT assay was used to determine cytotoxicity, Comet  assay was used to determine genotoxicity and,  DCFH-DA assay was used to determine oxidative stress values. Cells were exposed to two different doses of Cisplatin (11, 22 µM) for 24 hours. Cells were exposed to three different concentrations of RA (50, 100, 200 µM) both alone and in combination with two different doses of Cisplatin for 24 hours. As a result, it was determined, used RA concentrations have anti cytotoxic activity potential, against the cytotoxic effect increased by Cisplatin and antigenotoxic activity potential, against the genotoxic effect increased by Cisplatin. It was found that these activities of RA were based on antioxidant properties which reduced oxidative stress parameters increased by Cisplatin. In conclusion, the necessity to prevent the unconscious consumption of herbal products containing RA, especially in patients receiving Cisplatin chemotherapy has been shown.

Kaynakça

  • 1. Petersen M, Simmonds MSJ. Rosmarinic acid. Phytochemistry 2003; 62: 121–125.
  • 2. Scarpati ML, Oriente G. Isolamento e costituzione dell’acido rosmarinico (dal rosmarinus off.). La Ricerca Scientifica 1958; 28, 2329–2333.
  • 3. Rosmarinic acid, https://www.sigmaaldrich.com/catalog/product/aldrich /53 6954?lan g=en&region=TR&gclid=EAIaIQobChMIzNGusaXF3wIVz5IYCh3OWgwFEAAYASAAEgLBD_D_BwE (Erişim Tarihi 29.12.2018)
  • 4. Hiller K. Zur Kenntnis der Inhaltsstoffe einiger Saniculoidae. 1. Mitteilung: Sanicula europaea L.—Isolierung und quantitative Erfassung von Chlorogen-und Rosmarinsaure. Pharmazie 1965;20, 574–579.
  • 5. Trute A, Nahrstedt A. Separation of rosmarinic acid enantiomers by three different chromatographic methods (HPLC, CE, GC) and the determination of rosmarinic acid in Hedera helix L. Phytochem. Anal. 1986; 7, 204–208.
  • 6. De Tommasi N, De Simone, F, De Feo V, Pizza C. Phenylpropanoid Glycosides and Rosmarinic Acid from Momordica balsamina. Planta Med. 1991; 57, 201.
  • 7. Lasure A, Van Poel B, Pieters L, Claeys M, Gupta M, Vanden Berghe D, Vlietinck, AJ. Complement-inhibiting properties of Apeiba tibourbou. Planta Med. 1994; 60, 276–277.
  • 8. Aquino R, Ciavatta ML, De Simone F, Pizza C. A flavanone glycoside from Hamelia patens. Phytochemistry 1990; 29, 2358–2360.
  • 9. Satake T, Kamiya K., Saiki Y, Hama T, Fujimoto Y, Kitanaka S, Kimura Y, Uzawa J, Endang H, Umar M. Studies on the constituents of fruits of Helicteres isora L. Chem. Pharm. Bull. 1999; 47, 1444–1447.
  • 10. Ravn H, Pedersen MF, Andary J, Borum C, Anthoni U, Christophersen C, Nielsen PH. Seasonal variation and distribution of two phenolic compounds, rosmarinic acid and caffeic acid, in leaves and roots-rhizomes of eelgrass (Zostera marina L.). Ophelia 1994; 40, 51–61.
  • 11. Hausler E, Petersen M, Alfermann AW. Rosmarinsaure in Blechnum-Spezies. In: Haschke, H.P., Schnarrenberger, C. (Eds.), Botanikertagung. Berlin. Akademie Verlag, Berlin, p. 507. 1992.
  • 12. Takeda R, Hasegawa J, Sinozaki M. The first isolation of lignans, megacerotonic acid and anthocerotonic acid, from nonvascular plants, Anthocerotae (hornworts). Tetrahedron Lett. 1990; 31, 4159–4162.
  • 13. Chu X, Ci X, He J, Jiang L, Wei M, Cao Q, et al. Effects of a natural prolyl oligopeptidase inhibitor, rosmarinic acid, on lipopolysaccharide-induced acute lung injury in mice. Molecules. 2012;17:3586-98.
  • 14. Sánchez-Campillo M, Gabaldon JA, Castillo J, Benavente- García O, Del Baño MJ, Alcaraz M, et al. Rosmarinic acid, a photo-protective agent against UV and other ionizing radiations. Food Chem Toxicol. 2009; 47:386-92.
  • 15. Al-Dhabi N A, Arasu M V, Park CH, Park SU. Recent studies on rosmarinic acid and its biological and pharmacological activities.Excli Journal 2014;13:1192-1195.
  • 16. Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Research 2006. 40(2): 223-231.
  • 17. Saltan FZ, Canbay HS. Eskişehir’de Halk Arasında Kullanılan Bazı Bitkilerdeki Ağır Metal ve Besin Elementlerinin Belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2015. 19(1); 83-90
  • 18. İnci ve ark. New water-soluble copper (II) complexes including 4,7-dimethyl-1,10-phenanthroline and L-tyrosine: Synthesis, characterization, DNA interactions and cytotoxicities. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015; 136 ; 761–770.
  • 19. Roehm NW, Rodgers GH, Hatfield SM, Glasebrook AL. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 1991;13:142(2):257-65.
  • 20. Piperakis SM. Comet assay: A brief history. Cell Biol. Toxicol. 2009; 25:1–3.
  • 21. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1):184-91.
  • 22. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J Immunol. 1983; 130:1910-1917.
  • 23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983; 65: 55-63.
  • 24. Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989; 12;119(2): 203-210.
  • 25. Jost LM, Kirkwood JM, Whiteside TL. Improved short- and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells. J Immunol Methods. 1992; 4;147(2):153-65.
  • 26. Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol. 2014; 5, 0: 364–378.
  • 27. Manojkumar Y, Ambika S, Arulkumar R, Gowdhami B, Balaji P, Vignesh G, Arunachalam S, Marullo R, Werner E, Degtyareva N, Moore B, Altavilla G, Ramalingam SS, Doetsch PW. Cisplatin Induces a Mitochondrial-ROS Response That Contributes to Cytotoxicity Depending on Mitochondrial Redox Status and Bioenergetic Functions. Plos One 2013; 8(11), e81162.
  • 28. Liu Y, Song XQ, Li X, Liu X, Tian JL, Xu JY, Yan SP. Three pairs of enantiomers bearing mitochondria‐targetedTPP+groups as potential anti‐cancer agents. Appl. Organometal Chem. 2019; 33;e4920
  • 29. Zi CT, Yang L, Xu FQ, Dong FW, Yang D, Li Y, Ding ZT, Zhou J, Jiang ZH, Hu JM. Synthesis and anticancer activity of dimeric podophyllotoxin derivatives.Drug Desig, Development and Therapy. 2018; 12, 3393-3406.
  • 30. Apohan E, Yilmaz U, Yilmaz, Serindag A, Küçükbay H, Yesilada O, Baran Y. Synthesis, cytotoxic and antimicrobial activities of novel cobalt and zinc complexes of benzimidazole derivatives. Journal of Organometalic Chemistry.2017; 828, 52-58.
  • 31. Escola A, Crespo M, Lopez C, Quirante J, Jayaraman A, Polat IH, Badia J, Baldoma L, Cascante M. On teh stability and biological behavior of cyclometallated Pt(IV) complexes with hlido and aryl ligands in the axial positions. Bioorganic & Medicinal Chemistry. 2016; 24, 5804-5815.
  • 32. Bahri S, Miles F, Ali BR, Mlika M, Jameleddine S, Mc Entee K, Shlyonsky V. Rosmarinic acid potentiates carnosic acid induced apoptosis in lung fibroblasts. Plos ONE. 2017; 12(9); e0184368.
  • 33. Fialova SB, kello M, Coma M, Slobodnikova L, Drobna E, Holkova I, Garajova M, Mrva M, Zachar V, Lukac M. Derivatization of Rosmarinic Acid Enhances its in vitro Antitumor, Antimicrobial and Antiprotozoal Properties. Molecules 2019; 24(6), 1078.
  • 34. Juming L, Li Z, Xinyi Z, Yingying L, Fang Z, Chunsong Y, Wei Z. Protective effects and active ingredients of Salvia miltiorrhiza Bunge extractson airway responsiveness, inflammation and remodeling in mice withovalbumin-induced allergic asthma. Phytomedicine. 2019. 52; 168-177.
  • 35. Saad SY, Najjar TA, Alashari M. Role of non-selective adenosine receptor blockade and phosphodiesterase inhibition in cisplatin-induced nephrogonadal toxicity in rats.Clin.Exp.Pharmacol.Physiol. 2004; 31, 862-867.
  • 36. Dehne N, Lautermann J, Petrat F, Rauen U, de Groot H. Cisplatin Ototoxicity: Involvement of Iron and Enhanced Formation of Superoxide Anion Radicals. Toxicol Appl Pharmacol. 2001; 174, 27-34.
  • 37. Santos NA, Catão CS, Martins NM, Curti C, Bianchi ML, Santos AC. Cisplatin-induced nephrotoxicity is associated with oxidative stress, redox state unbalance, impairment of energetic metabolism and apoptosis in rat kidney mitochondria. Arch Toxicol. 2007; 81, 495-504.
  • 38. Jiang Y, Guo C, Vasko MR, Kelley MR. Implications of Apurinic/ Apyrimidinic Endonuclease in Reactive Oxygen Signaling Response after Cisplatin Treatment of Dorsal Root Ganglion Neurons. Cancer Res 2008; 68, 6425-6434.
  • 39. De Oliveira NCD, Sarmento MS, Nunes EA, Porto CM, Rosa DP, Bona SR, Rodrigues G, Marroni NP, Pereira P, Picada JN, Ferraz ABF, Thiesen FV, Da Silva J. Rosmarinic acit as a protective agents against genotoxicity of ethanol in mice. Food and Chemical Toxicology 2012; 50, 1208-1214.
  • 40. Kim DH, Lee JH. Comparative evaluation of phenolic phytochemicals from perilla seedsof diverse species and screening for their tyrosinase inhibitory andantioxidant properties. South African Journal of Botany 2019; 123, 341-350.
  • 41. Vergine M, Nicoli F, Negro C, Luvisi A, Nutricati E, Accogli RA, Sabena E, Miceli A. Phytochemical Profiles and Antioxidant Activity of Salvia species from Southern Italy. Records of Natural Products 2019; 13(3), 205-215.
  • 42. Erkan N, Ayranci G, Ayranc, E. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chemistry 2008; 110, 76-82.
  • 43. Ferguson LR, bronzetti G, De Flora S. Mechanistic approaches to chemoprevention of mutation and cancer. Mutat. Res. 2005; 591, 3-7.
  • 44. Venkatachalam K, Gunasekaran S, Jesudoss VAS, Namasivayam N. The Effect of rosmarinic acid on 1,2- dimethylhydrazine induced colon carcinogenesis. Experimental and Toxicologic Pathology 2013; 65, 409-418.
  • 45. https://www.sabah.com.tr/webtv/sifali-bitkiler/biberiye-rosmarinus-officinalis-nelere iyi-gelir-biberiyenin-rosmarinus-officinalis-faydalari-nelerdir (Erişim Tarihi; 05.05.2018)

Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması

Yıl 2019, Cilt: 45 Sayı: 3, 263 - 270, 01.12.2019
https://doi.org/10.32708/uutfd.613912

Öz

Rosmarinik Asit (RA) birçok bitki türünde bulunan önemli bir fenolik bileşiktir. Çalışmada RA’nın, A549 (İnsan Akciğer Kanser Hücre Hattı) ve Beas-2B (İnsan Sağlıklı Bronşial Epitel Hücre Hattı)  hücrelerinde, Cisplatin tarafından oluşturulan sitotoksik, genotoksik etki ve oksidatif strese karşın etkinliğinin in vitro olarak belirlenmesi ve böylelikle Cisplatin kemoterapisi sırasında hastalar tarafından kullanılabilecek RA içerikli bitkisel ürünlerin Cisplatin tedavisi üzerindeki olası etkilerinin belirlenmesi amaçlanmıştır. Bu amaca yönelik olarak sitotoksite belirlenmesinde XTT, genotoksite belirlenmesinde Komet ve oksidatif stres değerlerinin belirlenmesinde ise DCFH-DA yöntemleri kullanılmıştır. Yöntemlerde hücreler Cisplatin’in iki farklı dozuna (11, 22 µM) 24 saat süre ile maruz bırakılmıştır. Hücreler RA’nın üç farklı konsantrasyonuna (50, 100, 200 µM) hem tek başına hem de Cisplatin’in iki farklı dozu ile beraber uygulanarak 24 saat süre ile maruz bırakılmıştır. Sonuç olarak Cisplatin dozları tarafından arttırılan; sitotoksik etkiye karşın RA’nın kullanılan konsantrasyonlarının antisitotoksik aktivite, genotoksik etkiye karşın ise antigenotoksik aktivite gösterme potansiyeli olduğu belirlenmiştir. RA’nın bu aktivitelerinin temelinde de Cisplatin tarafından arttırılan oksidatif stres parametrelerini azaltıcı antioksidan özellik göstermesi olduğu belirlenmiştir. Sonuç olarak RA içeren bitkisel ürünlerin, özellikle Cisplatin kemoterapisi alan hastalarda bilinçsizce tüketiminin önlenmesi gerekliliği gösterilmiştir.

Kaynakça

  • 1. Petersen M, Simmonds MSJ. Rosmarinic acid. Phytochemistry 2003; 62: 121–125.
  • 2. Scarpati ML, Oriente G. Isolamento e costituzione dell’acido rosmarinico (dal rosmarinus off.). La Ricerca Scientifica 1958; 28, 2329–2333.
  • 3. Rosmarinic acid, https://www.sigmaaldrich.com/catalog/product/aldrich /53 6954?lan g=en&region=TR&gclid=EAIaIQobChMIzNGusaXF3wIVz5IYCh3OWgwFEAAYASAAEgLBD_D_BwE (Erişim Tarihi 29.12.2018)
  • 4. Hiller K. Zur Kenntnis der Inhaltsstoffe einiger Saniculoidae. 1. Mitteilung: Sanicula europaea L.—Isolierung und quantitative Erfassung von Chlorogen-und Rosmarinsaure. Pharmazie 1965;20, 574–579.
  • 5. Trute A, Nahrstedt A. Separation of rosmarinic acid enantiomers by three different chromatographic methods (HPLC, CE, GC) and the determination of rosmarinic acid in Hedera helix L. Phytochem. Anal. 1986; 7, 204–208.
  • 6. De Tommasi N, De Simone, F, De Feo V, Pizza C. Phenylpropanoid Glycosides and Rosmarinic Acid from Momordica balsamina. Planta Med. 1991; 57, 201.
  • 7. Lasure A, Van Poel B, Pieters L, Claeys M, Gupta M, Vanden Berghe D, Vlietinck, AJ. Complement-inhibiting properties of Apeiba tibourbou. Planta Med. 1994; 60, 276–277.
  • 8. Aquino R, Ciavatta ML, De Simone F, Pizza C. A flavanone glycoside from Hamelia patens. Phytochemistry 1990; 29, 2358–2360.
  • 9. Satake T, Kamiya K., Saiki Y, Hama T, Fujimoto Y, Kitanaka S, Kimura Y, Uzawa J, Endang H, Umar M. Studies on the constituents of fruits of Helicteres isora L. Chem. Pharm. Bull. 1999; 47, 1444–1447.
  • 10. Ravn H, Pedersen MF, Andary J, Borum C, Anthoni U, Christophersen C, Nielsen PH. Seasonal variation and distribution of two phenolic compounds, rosmarinic acid and caffeic acid, in leaves and roots-rhizomes of eelgrass (Zostera marina L.). Ophelia 1994; 40, 51–61.
  • 11. Hausler E, Petersen M, Alfermann AW. Rosmarinsaure in Blechnum-Spezies. In: Haschke, H.P., Schnarrenberger, C. (Eds.), Botanikertagung. Berlin. Akademie Verlag, Berlin, p. 507. 1992.
  • 12. Takeda R, Hasegawa J, Sinozaki M. The first isolation of lignans, megacerotonic acid and anthocerotonic acid, from nonvascular plants, Anthocerotae (hornworts). Tetrahedron Lett. 1990; 31, 4159–4162.
  • 13. Chu X, Ci X, He J, Jiang L, Wei M, Cao Q, et al. Effects of a natural prolyl oligopeptidase inhibitor, rosmarinic acid, on lipopolysaccharide-induced acute lung injury in mice. Molecules. 2012;17:3586-98.
  • 14. Sánchez-Campillo M, Gabaldon JA, Castillo J, Benavente- García O, Del Baño MJ, Alcaraz M, et al. Rosmarinic acid, a photo-protective agent against UV and other ionizing radiations. Food Chem Toxicol. 2009; 47:386-92.
  • 15. Al-Dhabi N A, Arasu M V, Park CH, Park SU. Recent studies on rosmarinic acid and its biological and pharmacological activities.Excli Journal 2014;13:1192-1195.
  • 16. Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Research 2006. 40(2): 223-231.
  • 17. Saltan FZ, Canbay HS. Eskişehir’de Halk Arasında Kullanılan Bazı Bitkilerdeki Ağır Metal ve Besin Elementlerinin Belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2015. 19(1); 83-90
  • 18. İnci ve ark. New water-soluble copper (II) complexes including 4,7-dimethyl-1,10-phenanthroline and L-tyrosine: Synthesis, characterization, DNA interactions and cytotoxicities. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015; 136 ; 761–770.
  • 19. Roehm NW, Rodgers GH, Hatfield SM, Glasebrook AL. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 1991;13:142(2):257-65.
  • 20. Piperakis SM. Comet assay: A brief history. Cell Biol. Toxicol. 2009; 25:1–3.
  • 21. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1):184-91.
  • 22. Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J Immunol. 1983; 130:1910-1917.
  • 23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983; 65: 55-63.
  • 24. Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989; 12;119(2): 203-210.
  • 25. Jost LM, Kirkwood JM, Whiteside TL. Improved short- and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells. J Immunol Methods. 1992; 4;147(2):153-65.
  • 26. Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol. 2014; 5, 0: 364–378.
  • 27. Manojkumar Y, Ambika S, Arulkumar R, Gowdhami B, Balaji P, Vignesh G, Arunachalam S, Marullo R, Werner E, Degtyareva N, Moore B, Altavilla G, Ramalingam SS, Doetsch PW. Cisplatin Induces a Mitochondrial-ROS Response That Contributes to Cytotoxicity Depending on Mitochondrial Redox Status and Bioenergetic Functions. Plos One 2013; 8(11), e81162.
  • 28. Liu Y, Song XQ, Li X, Liu X, Tian JL, Xu JY, Yan SP. Three pairs of enantiomers bearing mitochondria‐targetedTPP+groups as potential anti‐cancer agents. Appl. Organometal Chem. 2019; 33;e4920
  • 29. Zi CT, Yang L, Xu FQ, Dong FW, Yang D, Li Y, Ding ZT, Zhou J, Jiang ZH, Hu JM. Synthesis and anticancer activity of dimeric podophyllotoxin derivatives.Drug Desig, Development and Therapy. 2018; 12, 3393-3406.
  • 30. Apohan E, Yilmaz U, Yilmaz, Serindag A, Küçükbay H, Yesilada O, Baran Y. Synthesis, cytotoxic and antimicrobial activities of novel cobalt and zinc complexes of benzimidazole derivatives. Journal of Organometalic Chemistry.2017; 828, 52-58.
  • 31. Escola A, Crespo M, Lopez C, Quirante J, Jayaraman A, Polat IH, Badia J, Baldoma L, Cascante M. On teh stability and biological behavior of cyclometallated Pt(IV) complexes with hlido and aryl ligands in the axial positions. Bioorganic & Medicinal Chemistry. 2016; 24, 5804-5815.
  • 32. Bahri S, Miles F, Ali BR, Mlika M, Jameleddine S, Mc Entee K, Shlyonsky V. Rosmarinic acid potentiates carnosic acid induced apoptosis in lung fibroblasts. Plos ONE. 2017; 12(9); e0184368.
  • 33. Fialova SB, kello M, Coma M, Slobodnikova L, Drobna E, Holkova I, Garajova M, Mrva M, Zachar V, Lukac M. Derivatization of Rosmarinic Acid Enhances its in vitro Antitumor, Antimicrobial and Antiprotozoal Properties. Molecules 2019; 24(6), 1078.
  • 34. Juming L, Li Z, Xinyi Z, Yingying L, Fang Z, Chunsong Y, Wei Z. Protective effects and active ingredients of Salvia miltiorrhiza Bunge extractson airway responsiveness, inflammation and remodeling in mice withovalbumin-induced allergic asthma. Phytomedicine. 2019. 52; 168-177.
  • 35. Saad SY, Najjar TA, Alashari M. Role of non-selective adenosine receptor blockade and phosphodiesterase inhibition in cisplatin-induced nephrogonadal toxicity in rats.Clin.Exp.Pharmacol.Physiol. 2004; 31, 862-867.
  • 36. Dehne N, Lautermann J, Petrat F, Rauen U, de Groot H. Cisplatin Ototoxicity: Involvement of Iron and Enhanced Formation of Superoxide Anion Radicals. Toxicol Appl Pharmacol. 2001; 174, 27-34.
  • 37. Santos NA, Catão CS, Martins NM, Curti C, Bianchi ML, Santos AC. Cisplatin-induced nephrotoxicity is associated with oxidative stress, redox state unbalance, impairment of energetic metabolism and apoptosis in rat kidney mitochondria. Arch Toxicol. 2007; 81, 495-504.
  • 38. Jiang Y, Guo C, Vasko MR, Kelley MR. Implications of Apurinic/ Apyrimidinic Endonuclease in Reactive Oxygen Signaling Response after Cisplatin Treatment of Dorsal Root Ganglion Neurons. Cancer Res 2008; 68, 6425-6434.
  • 39. De Oliveira NCD, Sarmento MS, Nunes EA, Porto CM, Rosa DP, Bona SR, Rodrigues G, Marroni NP, Pereira P, Picada JN, Ferraz ABF, Thiesen FV, Da Silva J. Rosmarinic acit as a protective agents against genotoxicity of ethanol in mice. Food and Chemical Toxicology 2012; 50, 1208-1214.
  • 40. Kim DH, Lee JH. Comparative evaluation of phenolic phytochemicals from perilla seedsof diverse species and screening for their tyrosinase inhibitory andantioxidant properties. South African Journal of Botany 2019; 123, 341-350.
  • 41. Vergine M, Nicoli F, Negro C, Luvisi A, Nutricati E, Accogli RA, Sabena E, Miceli A. Phytochemical Profiles and Antioxidant Activity of Salvia species from Southern Italy. Records of Natural Products 2019; 13(3), 205-215.
  • 42. Erkan N, Ayranci G, Ayranc, E. Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chemistry 2008; 110, 76-82.
  • 43. Ferguson LR, bronzetti G, De Flora S. Mechanistic approaches to chemoprevention of mutation and cancer. Mutat. Res. 2005; 591, 3-7.
  • 44. Venkatachalam K, Gunasekaran S, Jesudoss VAS, Namasivayam N. The Effect of rosmarinic acid on 1,2- dimethylhydrazine induced colon carcinogenesis. Experimental and Toxicologic Pathology 2013; 65, 409-418.
  • 45. https://www.sabah.com.tr/webtv/sifali-bitkiler/biberiye-rosmarinus-officinalis-nelere iyi-gelir-biberiyenin-rosmarinus-officinalis-faydalari-nelerdir (Erişim Tarihi; 05.05.2018)
Toplam 45 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Klinik Tıp Bilimleri (Diğer)
Bölüm Özgün Araştırma Makaleleri
Yazarlar

Özgür Vatan 0000-0002-7687-3284

Yayımlanma Tarihi 1 Aralık 2019
Kabul Tarihi 25 Eylül 2019
Yayımlandığı Sayı Yıl 2019 Cilt: 45 Sayı: 3

Kaynak Göster

APA Vatan, Ö. (2019). Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması. Uludağ Üniversitesi Tıp Fakültesi Dergisi, 45(3), 263-270. https://doi.org/10.32708/uutfd.613912
AMA Vatan Ö. Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması. Uludağ Tıp Derg. Aralık 2019;45(3):263-270. doi:10.32708/uutfd.613912
Chicago Vatan, Özgür. “Rosmarinik Asidin Cisplatine Karşı Antisitotoksik Ve Antigenotoksik Etkisinin A549 Ve Beas-2B Hücre Hatlarında Araştırılması”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 45, sy. 3 (Aralık 2019): 263-70. https://doi.org/10.32708/uutfd.613912.
EndNote Vatan Ö (01 Aralık 2019) Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması. Uludağ Üniversitesi Tıp Fakültesi Dergisi 45 3 263–270.
IEEE Ö. Vatan, “Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması”, Uludağ Tıp Derg, c. 45, sy. 3, ss. 263–270, 2019, doi: 10.32708/uutfd.613912.
ISNAD Vatan, Özgür. “Rosmarinik Asidin Cisplatine Karşı Antisitotoksik Ve Antigenotoksik Etkisinin A549 Ve Beas-2B Hücre Hatlarında Araştırılması”. Uludağ Üniversitesi Tıp Fakültesi Dergisi 45/3 (Aralık 2019), 263-270. https://doi.org/10.32708/uutfd.613912.
JAMA Vatan Ö. Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması. Uludağ Tıp Derg. 2019;45:263–270.
MLA Vatan, Özgür. “Rosmarinik Asidin Cisplatine Karşı Antisitotoksik Ve Antigenotoksik Etkisinin A549 Ve Beas-2B Hücre Hatlarında Araştırılması”. Uludağ Üniversitesi Tıp Fakültesi Dergisi, c. 45, sy. 3, 2019, ss. 263-70, doi:10.32708/uutfd.613912.
Vancouver Vatan Ö. Rosmarinik Asidin Cisplatine Karşı Antisitotoksik ve Antigenotoksik Etkisinin A549 ve Beas-2B Hücre Hatlarında Araştırılması. Uludağ Tıp Derg. 2019;45(3):263-70.

ISSN: 1300-414X, e-ISSN: 2645-9027

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