Total Kolesterol, HDL-kolesterol, Trigliserid, Hesaplanmış LDL-kolesterol ve HDL dışı-kolesterol için Ölçüm Belirsizliği

Yıl 2022, Cilt: 48 Sayı: 1, 19 - 23, 01.04.2022

Şeniz Korkmaz

https://doi.org/10.32708/uutfd.1057697

Öz

Ölçüm belirsizliği, bir sonucun kalitesinin kantitatif bir göstergesi olup, ölçüm sonuçlarının güvenilirlik düzeyinde gerçekleştirildiğini gösterir. Çalışmamızda laboratuvarımızda sık istenen testlerden olan total kolesterol (TK), HDL-kolesterol (HDL-K), trigliserid (Tg), hesaplanmış LDL-kolesterol (LDL-K) ve HDL dışı-kolesterol (HDL dışı-K) için ölçüm belirsizliğini hesaplamak ve bu değerleri CLIA 2019 toplam izin verilen hata (%TEa) değerleriyle karşılaştırmak amaçlandı. Çalışmamız Karacabey Devlet Hastanesi Merkez Laboratuvarı’nda gerçekleştirildi. Nordtest kılavuzunda tanımlanan ve 6 adımdan oluşan hesaplama modeli ile ölçüm belirsizliği belirlendi. Mart-Ağustos 2020 dönemine ait iç ve dış kalite kontrol verileri kullanıldı. Ölçüm belirsizliği, TK, HDL-K ve Tg için %7, LDL-K için %12, HDL dışı-K için %10 olarak hesaplandı. TK, HDL-K ve Tg testleri için hesaplanan ölçüm belirsizliği, hedef %TEa değerinin altındadır. Ölçüm belirsizliğinin belirlenmesi ve düzenli olarak izlenmesi, laboratuvar içinde doğru sonuçlar üretmeyi ve kullanıcılara sonuçları yorumlamak için objektif bir araç sağlamayı amaçlar.

Anahtar Kelimeler

toplam izin verilen hata, analitik faz, ölçüm belirsizliği, medikal laboratuvar, kalite kontrol

Teşekkür

Prof. Dr. Esma Gür, Prof. Dr. Arzu Yılmaztepe Oral ve laboratuvar teknik personeline katkılarından dolayı teşekkür ederiz.

Measurement Uncertainty for Total Cholesterol, HDL-cholesterol, Triglycerides, Calculated LDL-cholesterol and non-HDL-cholesterol

Öz

Measurement uncertainty is a quantitative indicator of the quality of a result, indicates that the measurement results are performed at the level of reliability. It was aimed to calculate the measurement uncertainty for total cholesterol, HDL-cholesterol, triglycerides, calculated LDL-cholesterol, and non-HDL-cholesterol, which are frequently ordered tests in our laboratory, and to compare these values with CLIA 2019 allowable total error (TEa%) values. Our study was performed in the Central Laboratory of Karacabey State Hospital. The measurement uncertainty was determined with the calculation model defined in the Nordtest guide consisting of 6 steps. Internal and external quality control data were recorded from March to August 2020. The measurement uncertainty was calculated as 7% for total cholesterol, HDL-cholesterol and triglycerides, 12% for LDL-cholesterol and 10% for non-HDL-cholesterol. Measurement uncertainty for total cholesterol, HDL-cholesterol and triglycerides was found below the targeted TEa%. Measurement uncertainty estimation and its periodical verification aim to produce accurate results within the laboratory and provide users with an objective tool to interpret the results.

Anahtar Kelimeler

allowable total error, analytical phase, measurement uncertainty, medical laboratory, quality control

Kaynakça

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