Protective effect of Vaccinium myrtillus on Paraoxonase Activity, Some Biochemical Parameters and Pancreatic Functions in Diabetic Rats

Öz

Objective: Diabetes is one of the biggest problems in the field of public health in the world. Vaccinium myrtillus L. extract, among the medicinal plants, has powerful antioxidant activity. For this aim, the possible effects of Vacillus myrtillus extract on serum paraoxonase (PON1), pancreatic enzyme levels, and lipid levels in experimental diabetic rats were investigated. Material and Method: A total of 32 male rats (Wistar albino) were used in this study and divided into four groups. Control (C), diabetic (D), Vaccinium myrtillus (VM), diabetes plus Vaccinium myrtillus (DVM) groups. In D and DVM groups, experimental diabetes was induced using a single dose of intraperitoneal 45 mg/kg streptozotocin. Vaccinium myrtillus extract was administered as 1.2 mg/kg/21 days by oral gavage in VM and DVM groups. At the end of the study; serum HbA1c, lipid parameters, amylase, lipase, and paraoxonase (PON1) enzymes were analyzed. Results: HbA1c was significantly reduced in diabetic groups treated with VM (VM and DVM). Cholesterol, LDL-C, and VLDL-C levels were significantly reduced after VM was administered to diabetic subjects, while other lipids were found to decrease insignificantly. Vaccinium myrtillus extract caused a slight decrease in pancreatic enzymes and a statistically significant increase in paraoxonase enzyme in the DVM group compared to group D. Conclusion: The obtained data support that Vaccinium myrtillus extract may play a potential protective role in preventing diabetes-associated complications.

Anahtar Kelimeler

• Vaccinium mrytillus
• diabetes
• paraoxonase
• pancreas function
• lipids

Diyabetik Sıçanlarda Vaccinium myrtillus'un Paraoksonaz Aktivitesi, Bazı Biyokimyasal Parametreler ve Pankreas Fonksiyonları Üzerindeki Koruyucu Etkisi

Öz

Amaç: Diyabet, dünyada halk sağlığı alanında en büyük sorunlardan biridir. Şifalı bitkiler arasında yer alan Vaccinium myrtillus L. özütü, güçlü antioksidan aktiviteye sahiptir. Bu amaçla, deneysel diyabetik sıçanlarda Vaccinium myrtillus ekstraktının serum paraoksonaz (PON1), pankreas enzim düzeyleri ve lipid düzeyleri üzerine olası etkileri araştırıldı. Gereç ve Yöntem: Bu çalışmada toplam 32 erkek sıçan (Wistar albino) kullanıldı ve dört gruba ayrıldı. Kontrol(C), diyabetik (D), Vaccinium myrtillus (VM), diyabet artı Vaccinium myrtillus (DVM) grupları. D ve DVM gruplarında, tek doz intraperitoneal 45 mg/kg streptozotosin kullanılarak deneysel diyabet oluşturuldu. Vaccinium myrtillus özütü, VM ve DVM gruplarına oral gavaj yoluyla 1.2 mg/kg/21 gün olarak uygulandı. Çalışmanın sonunda; serum HbA1c, lipid parametreleri, amilaz, lipaz ve paraoksonaz (PON1) enzimleri analiz edildi. Bulgular: VM (VM ve DVM) ile tedavi edilen diyabetik gruplarda HbA1c önemli ölçüde azaldı. Diyabetik deneklere VM uygulandıktan sonra kolesterol, LDL-C ve VLDL-C seviyeleri önemli ölçüde azalırken, diğer lipidlerin önemsiz bir şekilde azaldığı bulundu. Vaccinium myrtillus ekstresi DVM grubunda D grubuna göre pankreas enzimlerinde hafif bir düşüşe ve paraoksonaz enziminde istatistiksel olarak anlamlı bir artışa neden oldu. Sonuç: Elde edilen veriler, Vaccinium myrtillus ekstresinin diyabetle ilişkili komplikasyonların önlenmesinde potansiyel koruyucu bir rol oynayabileceğini desteklemektedir.

Anahtar Kelimeler

• Vaccinium mrytillus
• diyabet
• paraoksonaz
• pankreas fonksiyonu
• lipidler

Kaynakça

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