ÜREME SEZONUNDA SENKRONİZE EDİLEN KEÇİLERDE DONDURULMUŞ SPERMA İLE FARKLI ZAMANLARDA YAPILAN SERVİKAL TOHUMLAMALARIN GEBELİK ORANLARINA ETKİSİ

Yıl 2021, Cilt: 14 Sayı: 1, 41 - 49, 30.04.2021

Fikret Karaca , Nurdan Coşkun Çetin , Haydar Demirezer Oğuz Kaan Yalçın , Cafer Tayyar Ateş

https://doi.org/10.52976/vansaglik.775562

Cited By: 1

Öz

Çalışmada üreme mevsimindeki keçilerde vaginal sünger uygulaması ile senkronizasyon sonrası farklı zamanlarda gerçekleştirilen intra servikal tohumlamaların başarısı değerlendirildi. Çalışmada yaşları 2-5 arasında değişen, en az bir doğum yapmış, klinik olarak sağlıklı toplam 120 baş Damascus ırkı keçi kullanıldı. Keçiler tesadüfi olarak 4 eşit gruba ayrıldı (n=30). Keçilere senkronizasyon amacıyla progesteron içeren vaginal süngerler takıldı ve 9 gün boyunca vaginada tutuldu. Süngerlerin çıkarılması esnasında 200 IU PMSG ve 250 mcg PGF2α uygulandı. Suni tohumlama uygulamaları vajinal süngerlerlerin çıkarılmasını izleyen 44-48. (Grup I), 48-52. (Grup II), 52-56. (Grup III) ve 56-60. (Grup IV) saatler arasında Saanen ırkı tekelere ait dondurulmuş ithal sperma payetleri ile intraservikal olarak gerçekleştirildi. Suni tohumlama uygulamalarından 21 gün sonra kan örnekleri alınarak gebeliklerin teşhisi için progesteron seviyesine bakıldı, ayrıca 50. günlerde ultrasonografik görüntüleme ile gebeliklerin tayini yapıldı. 21. ve 50. günlerdeki gebelik oranları sırasıyla % 40.0, % 26.6; % 23.3, % 20.0; % 36.6, % 20 ve son grupta % 30, % 23.3 olarak belirlendi. Abort oranları sırasıyla % 12.5, % 16.6, % 16.6 ve % 14.3 olarak kaydedildi. Doğum ve fertilite oranları sırasıyla grup I’de % 87.5, % 23.3; grup II’de % 83.3, % 16.6; grup III’de % 83.3, % 16.6 ve grup IV’de % 85.7, % 20.0 olarak belirlendi. Fertilite parametreleri bakımından gruplar arasında fark bulunmaması nedeniyle dondurulmuş-çözdürülmüş sperma kullanılarak yapılan intraservikal tohumlamaların vaginal süngerlerin çekilmesini takiben 44-60. saatler arasında yapılabileceği kanaatine varıldı.

Anahtar Kelimeler

DONDURULMUŞ SPERMA, SUNİ TOHUMLAMA, SENKRONİZASYON, KEÇİ

Destekleyen Kurum

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

18.YL.020 (Münferit Proje)

Teşekkür

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından 18.YL.020 nolu Münferit Projesi olarak desteklenmiştir.

Kaynakça

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