miRNA’lar: Biyogenezi, Analiz Yöntemleri ve Biyobelirteç Potansiyeli

Yıl 2022, Cilt: 15 Sayı: 1, 95 - 102, 30.04.2022

İrem Gülfem Albayrak

https://doi.org/10.52976/vansaglik.952243

Öz

miRNA’lar translasyon sonrası gen anlatımının düzenlenmesinde görev alan, yaklaşık 22 nükleotit uzunluğundaki kodlama yapmayan küçük RNA molekülleridir. Hedef mRNA’ya bağlanan miRNA’lar translasyonun baskılanmasına veya mRNA’nın degredasyonuna neden olurlar. Hücre çoğalması, farklılaşması ve sağ kalımında rol oynayan miRNA’ların çeşitli hastalıklar ile ilişkili mekanizmalarda da yer aldıkları bilinmektedir. Hastalıklarla bağlantılı miRNA’ların belirlenmesinin çeşitli hastalıkların moleküler mekanizmasını anlama ve tedavi etmede daha güvenilir moleküler hedefler sağlayabileceği düşünülmektedir. Bu derleme çalışmasında miRNA’ların biyogenezi, izolasyon yöntemleri, hedef mRNA’ların belirlenmesi için kullanılan teknikler ile miRNA’ların çeşitli hastalıklardaki rollerinden bahsedilmiştir.

Anahtar Kelimeler

miRNA, Gen Anlatımı, Biyobelirteç

Kaynakça

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