Bromelain has Antioxidant Effect on Methotrexate Hepatotoxicity and Nephrotoxicity

Yıl 2022, Cilt: 15 Sayı: 1, 37 - 42, 30.04.2022

Ali Gürel Kürşat Kaya

https://doi.org/10.52976/vansaglik.982411

Cited By: 1

Öz

Objective: The goal of this study was to look into whether bromelain (BRM) could protect against methotrexate (MTX)-induced kidney and liver damage.
Material and Method: The rats were divided into four groups (n=7); the control group was given distilled water by gavage for 14 days and intraperitoneal (i.p.) physiological saline (%0.9 NaCI) on the third day; the BRM group was given 200 mg/kg BRM by gavage for 14 days and i.p. physiological saline (%0.9 NaCI) on third day; the MTX group was given distilled water by gavage for 14 days and i.p. single dose of 20 mg/kg MTX on the third day; the MTX+BRM group was given 200 mg/kg BRM by gavage for 14 days and i.p. single dose of 20 mg/kg MTX on the third day. Rats were decapitated at the end of the experiment, kidney and liver tissues were kept at -80°C, and biochemical analyzes were performed on the supernatants obtained from tissue homo-gentates.
Results: With the administration of MTX, oxidation indicator thiobarbituric acid reactive substance (TBARS) levels increased in comparison with the control group; antioxidants- glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) activities were decreased compared to the control group. Co-administration of BRM with MTX resulted in reduction in TBARS level and increase in GSH, CAT, SOD, and GPx activities.
Conclusion: In this study, it was determined that MTX caused oxidative damage in kidney and liver tissues and BRM prevented this damage.

Anahtar Kelimeler

Methotrexate, Oxidative damage, Kidney, Liver, Bromelain

Bromelain Metotreksat Hepatotoksisitesi ve Nefrotoksisitesi Üzerine Antioksidan Etkilidir

Öz

Amaç: Bu çalışmada bromelainin (BRM) metotreksat (MTX) kaynaklı böbrek ve karaciğer hasarına karşı potansiyel koruyucu etkilerinin araştırılması amaçlandı.
Materyal ve Metot: Dört gruba ayrılan sıçanlarda (n=7); kontrol grubuna 14 gün gavaj yoluyla distile su ve üçüncü gün intraperitoneal (i.p.) fizyolojik salin (%0.9 NaCI) verildi; BRM grubuna 14 gün boyunca gavaj yol-uyla 200 mg/kg BRM ve üçüncü gün i.p fizyolojik salin (%0.9 NaCI); MTX grubuna 14 gün boyunca gavaj yolu-yla distile su ve üçüncü günde i.p. tek doz 20 mg/kg MTX; BRM+MTX grubuna 14 gün boyunca gavaj yoluyla 200 mg/kg BRM ve üçüncü günde i.p. tek doz 20 mg/kg MTX uygulandı. Deney sonunda sıçanlar dekapite edildi, böbrek ve karaciğer dokuları -80 °C'de muhafaza edilerek, doku homojentlarından elde edilen superna-tantlarda biyokimyasal analizler gerçekleştirildi.
Bulgular: MTX uygulaması ile oksidasyon belirteci tiyobarbitürik asit reaktif madde (TBARS) seviyeleri kontrol grubuna kıyasla arttı; antioksidanlar süperoksit dismutaz (SOD), glutatyon (GSH), glutatyon peroksidaz (GPx) ve katalaz (CAT) aktiviteleri kontrol grubuna göre azaldı. BRM'nin MTX ile birlikte uygulanması, TBARS se-viyesinde azalmaya ve GSH, CAT, SOD ve GPx aktivitelerinde artışa neden oldu.
Sonuç: Bu çalışmada MTX'in böbrek ve karaciğer dokularında oksidatif hasara neden olduğu ve BRM'nin bu hasarı önlediği belirlenmiştir.

Anahtar Kelimeler

Metotreksat, Oksidatif hasar, Böbrek, Karaciğer, Bromelain.

Kaynakça

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