Artemisinin Uygulamasının, Pentilentetrazol ile İndüklenen Farelerin Karaciğer ve Böbrek Dokusunda Total Oksidan/Antioksidan ve Oksidatif Stres İndeksi Üzerine Etkisinin Araştırılması

Öz

Bu çalışma, pentilentetrazol (PTZ) uygulanan farelerde oluşabilecek oksidatif strese karşı artemisinin ön tedavisinin farelerin karaciğer ve böbrek dokularında toplam oksidan (TOS)/antioksidan (TAS) ve oksidatif stres indeksi (OSI) düzeylerindeki değişiklikleri araştırmak için tasarlanmıştır. Çalışmada İsviçre albino fareleri (Erkek) (n=42) kullanıldı. Fareler altı gruba ayrıldı ve her grupta yedi hayvan (n=7) vardı: (1) Kontrol (C) /salin Grubu, (2) PTZ (35 mg/kg.) Grubu, (3) Valproat (VPA) (100 mg/kg) + PTZ Grubu, (4) Artemisinin (ART) (30 mg/kg) + PTZ Grubu, (5) ART (60 mg/kg) + PTZ Grubu, (6) ART (120 mg/kg) + PTZ Grubu. Fareler, intraperitoneal (ip) olarak enjeksiyonlar uygulandı. Uygulamalardan sonra hayvanlar 30 dakika boyunca nöbetler için gözlendi. Deneyin son gününde (26. gün) farelere PTZ yükleme dozu (75 mg/kg) uygulandı ve ardından hayvanlar sakrifiye edildi. Karaciğer ve böbrek dokusunda TAS, TOS ve OSI düzeyleri ölçüldü. PTZ, karaciğer ve böbrek dokusunda TOS'u artırdı ve TAS'ı azalttı. ART, artan dozlarda karaciğer dokusunda TAS'ı önemli ölçüde artırdı ve TOS'u azalttı (p<0.05). ART böbrek dokusunda çok etkili değildi. Ancak böbrek dokusundaki TAS düzeyleri VPA diğer gruplarla karşılaştırıldığında anlamlı olarak yüksekti (p<0.05). Bu çalışmada, PTZ kaynaklı oksidatif stresin, periferik dokularda glutamat reseptörlerinin aktivasyonundan kaynaklanabileceği varsayılabilir. ART, PTZ'nin neden olduğu oksidatif stres ve hipoksiye bağlı karaciğer hasarına karşı koruyucu bir etkiye sahip olabilir. Bu etki, ART'nin antioksidan kapasitesine bağlı olabilir.

Anahtar Kelimeler

• Artemisinin
• Böbrek
• Karaciğer
• Oksidatif stres
• Pentilentetrazol

Investigation of The Effect of Artemisinin Administration on Total Oxidant/Antioxidant and Oxidative Stress-Index in The Liver and Kidney Tissue of Pentylenetetrazole-Induced Mice

Öz

This study was designed to investigate the changes in total oxidant (TOS)/antioxidant (TAS) and oxidative stress index (OSI) levels in liver and kidney tissues of mice pre-treatment of artemisinin against oxidative stress that may occur in mice administered pentylenetetrazole (PTZ). Swiss albino mice (Male) (n=42) were used in the study. The mice were divided into six groups and each group had seven animals (n=7): (1) Control (C) /saline Group, (2) PTZ (35 mg/kg) Group, (3) Valproate (VPA) (100 mg/kg) + PTZ Group, (4) Artemisinin (ART) (30 mg/kg) + PTZ Group, (5) ART (60 mg/kg) + PTZ Group, (6) ART (120 mg/kg) + PTZ Group. Mice received injections intraperitoneally (ip). After the treatments, the animals were observed for seizures for 30 minutes. On the last day (day 26) of the experiment, the PTZ loading dose (75 mg/kg) was administered to the mice and then the animals were sacrificed. TAS, TOS and OSI levels were measured in liver and kidney tissue. PTZ increased TOS and decreased TAS in liver and kidney tissue. ART significantly increased TAS and decreased TOS in liver tissue at increasing doses (p<0.05). ART was not very effective in kidney tissue. However, TAS levels in kidney tissue were significantly higher when VPA was compared with other groups (p<0.05). In this study, it can be assumed that PTZ-induced oxidative stress may be due to the activation of glutamate receptors in peripheral tissues. ART may have a protective effect against liver damage due to PTZ-induced oxidative stress and hypoxia. This effect may be due to the antioxidant capacity of ART.

Anahtar Kelimeler

• Artemisinin
• Kidney
• Liver
• Oxidative stress
• Pentylenetetrazole

Destekleyen Kurum

The authors declared thatthis study has received no financial support

Kaynakça

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