Different Application Techniques of Medical Ozone in The Treatment of Corneal Alkali Burns: Experimental Rabbit Model

Öz

The aim of this study is to clinically and histopathologically evaluate the efficiency of different applications of medical ozone on corneal healing in the treatment of experimentally induced corneal alkali burns with sodium hydroxide (NaOH) in rabbits. For this purpose, 6 mm diameter filter paper impregnated with 1 N NaOH was applied to the central cornea of only the right eyes of 32 New Zealand rabbits for 1 min. The rabbits were randomly divided into four groups. The treatment continued for 21 days at three-day intervals. The first group was left as the control group (K) and was not applied medical ozone. The control group received no treatment. Subconjunctival ozonated blood was applied to the second group (M). Subconjunctival medical ozone was applied to the third group (S). Major autohemotherapy was applied to the fourth group (O). As a result of clinical and histopathological evaluations, highest degree of regeneration and healing findings in corneal tissues were in the M group. This was followed by O and S groups, respectively. The highest corneal tissue damage was observed in the cases belonging to group K. The cases closest to normal stromal thickness belonged to group M. It was noted that the K group had the highest stromal thickness and the farthest value from normal. In the light of the clinical, statistical and histopathological findings, different application techniques of medical ozone treatment in corneal alkali burn were found to significantly reduce complications compared to the control group and accelerate corneal reepithelization.

Anahtar Kelimeler

• Burn
• Cornea
• Ozone
• Rabbit

Proje Numarası

TDK-2017-6269

Kornea Alkali Yanıklarının Sağaltımında Medikal Ozonun Farklı Uygulama Teknikleri: Deneysel Tavşan Modeli

Öz

Çalışmada tavşanlarda sodyum hidroksitle (NaOH) deneysel olarak oluşturulan kornea alkali yanıkların sağaltımında medikal ozonun farklı uygulama şekillerinin korneal iyileşme üzerine olan etkinliğinin klinik ve histopatolojik olarak incelenmesi amaçlandı. Bu amaçla 32 adet Yeni Zelanda ırkı tavşanların sadece sağ gözlerinin sentral korneasına 1 normal (N) NaOH emdirilmiş 6 mm çaplı filtre kâğıdı 1 dk süre ile uygulandı. Tavşanlar rastgele dört gruba ayrıldı. Sağaltım üçer (3) gün aralıklarla 21 gün boyunca devam etti. Birinci grup (K) kontrol grubu olarak bırakıldı. Yanık sonrası bu gruba herhangi tedavi uygulanmadı. İkinci gruba (M) subkonjuktival ozonlanmış kan uygulandı. Üçüncü gruba (S) subkonjunktival medikal ozon uygulaması yapıldı. Dördüncü gruba (O) major otohemoterapi uygulandı. Klinik ve histopatolojik değerlendirmeler neticesinde, kornea dokularında en yüksek derecede rejenerasyon ve iyileşme bulgularının M grubunda olduğunu bunu sırasıyla O grubu ve S grubu takip etti. En yüksek korneal doku hasarı K grubuna ait olgularda gözlendi. Normal stromal kalınlığa en yakın olgular M grubunda olup, K grubunun en yüksek stromal kalınlığa ve normalden en uzak değere sahip olduğu dikkati çekti. Elde edilen klinik, istatistiksel ve histopatolojik bulgular ışığında; korneal alkali yanığında medikal ozon sağaltımının farklı uygulama tekniklerinin, kontrol grubuna kıyasla oluşan komplikasyonları büyük oranda giderdiği ve korneal reepitelizasyonu hızlandırdığı görüldü.

Anahtar Kelimeler

• Kornea
• Ozon
• Tavşan
• Yanık

Destekleyen Kurum

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

TDK-2017-6269

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