The Relationship Between CACNA2D1 Gene and Subclinic Mastitis in Holstein Breed Cattle

Öz

The CACNA2D1 gene encodes the CACNA2D1 protein and, this protein is involved in the excitation-contraction mechanism of the muscle cells during milk withdrawal, helps the nipples to open and close. Because of this role in physiological mechanism and its relationship with quantitative trait locus (QTL) regions, CACNA2D1 gene is known to be associated with mastitis resistance. In this study, it was aimed to investigate the relationship between different three SNP (C367400T, A496561G and G519663A) on the CACNA2D1 gene, and subclinical mastitis in Holstein breed cattle reared in Develi district of Kayseri province. SNPs were genotyped from DNA samples by PCR-RFLP method. In the study, California mastitis test (CMT) data, and distributions of genotypes of the three SNPs on the CACNA2D1 gene were calculated. In the study, genotype distributions were determined in terms of C367400T, A496561G and G519663A SNPs found on the CACNA2D1 gene according to CMT status. The difference between the C367400T, A496561G and G519663A SNPs was not significant (p>0.05). In the study group examined the Chi-square (χ2) analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium (HWE) in terms of C367400T and A496561G SNPs, deviation from HWE for the G519663A SNP (p<0.05). As a result, it was thought that the CACNA2D1 gene and these SNPs should be evaluated with more samples and different mastitis indicator data in studies on mastitis resistance.

Anahtar Kelimeler

• CACNA2D1
• Bovine mastitis
• Cattle
• Genetic marker

Holştayn Irkı Sığırlarda CACNA2D1 Geni ve Subklinik Mastitis Arasındaki İlişki

Öz

CACNA2D1 geni, CACNA2D1 proteinini kodlar ve sütün indirilmesi sırasında kas hücrelerinin uyarılma-kasılma mekanizmasında görev alarak meme uçlarının açılıp kapanmasına yardımcı olur. Fizyolojik olarak üstelendiği bu görev ve kantitatif özellik lokusu (QTL) bölgelerine yakınlığı nedeni ile CACNA2D1 geninin mastitis direnci ile ilişkili olduğu bilinmektedir. Bu çalışmada Kayseri ili Develi ilçesinde yetiştirilen sağmal Holştayn sığırlarda CACNA2D1 geninde bulunan üç farklı SNP bölgesi ile subklinik mastitis durumları arasındaki ilişkinin araştırılması amaçlandı. Çalışma grubunu hepsi üçüncü laktasyonda 151 baş sağmal Holştayn sığır ırkı oluşturdu. Çalışmada California mastitis test (CMT) durumuna göre CACNA2D1 geni üzerinde bulanan üç SNP (C367400T, A496561G ve G519663A) yönünden genotip dağılımları belirlendi. Yapılan istatistiki analizler sonucunda C367400T, A496561G ve G519663A SNP genotipleri arasında fark bulunmadı (p>0.05). Yapılan Chi-Kare analizinde incelenen çalışma grubunda C367400T ve A496561G kodlu SNP’ler yönünden Hardy-Weinberg dengesinde (HWE) oldukları, G519663A kodlu SNP yönünden ise HWE’den saptıkları (P<0.05) gözlemlendi. Sonuç olarak mastitis direnci ile ilgili çalışmalarda CACNA2D1 geni ve bu SNP'lerin daha fazla örneklemle ve farklı mastitis göstergesi verilerle değerlendirilmesi gerektiği düşünüldü.

Anahtar Kelimeler

• CACNA2D1
• Genetik belirteç
• Sığır
• Sığır mastit

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