Geriatrik Ratlarda Bazı Böbrek Biyobelirteç Düzeyleri

Yıl 2023, , 213 - 218, 27.11.2023

Yıldıray Basbugan , Nazmi Yüksek , Ahmet Kömüroğlu , Eda Nur Okman , Ugur Ozdek

https://doi.org/10.36483/vanvetj.1273203

Cited By: 1

Öz

Bu çalışmada ileri yaşlı ratlarda, son yıllarda böbrek belirteci olarak sıkça kullanılan neutrophil gelatinase-associated lipocalin (NGAL), kidney ınjury molecule-1(KIM-1), sistatin C (Cyc-c) ve interleukin-18 (IL-18) düzeylerinin belirlenmesi ve bu düzeylerin genç ratlardakilerle kıyaslanması hedeflenmiştir. Bu amaçla çalışmamızda 12 Adet geriatrik Wistar albino rat (30-36 aylık) (6 erkek ve 6 dişi) ile 12 adet (2-3 aylık) (6 adet erkek ve 6 adet dişi) Wistar albino rat kullanıldı. Yaşlı ratların ortalama canlı ağırlıkları genç ratlara göre istatistiksel olarak önemli derecede yüksekti (p<0.001). Çıkartılan 24 saatlik idrar miktarı yaşlı ratlarda gençlere göre istatistiksel olarak yüksekti (p<0.01). Geriatrik ratlarda Serum Cyc-c (p<0.05), KIM-1 (p<0.01), glikoz (p<0.05), total protein (TP) (p<0.001) ve kreatinin (Crea) düzeyleri (p<0.001) istatistiksel olarak genç ratlarınkine göre daha yüksekti. Ancak IL-18, NGAL ve Üre (mg/dL) düzeylerinde istatistiksel olarak anlamlı bir fark bulunamadı. Geriatrik ratlarda, 24 saatlik idrar örneğinde Cyc-c (p<0.05), üre (p<0.01), Crea (p<0.01) ve Protein (p<0.001) düzeyleri genç ratlara göre istatistiksel olarak anlamlı olmakla birlikte; KIM-1, IL-18 ve NGAL düzeylerinde istatistiksel olarak anlamlı fark yoktu. Sonuç olarak geriatrik ratlarda KIM-1, Cyc-C değişikliklerinin yanı sıra Crea, glucose, urea, TP düzeyleri dikkate alınarak tanı, tedavi ve prognoz değerlendirilmelidir. Ayrıca kan Crea, glukoz, üre, TP düzeylerinin yanı sıra KIM-1, Cyc-C düzeyleri ve idrar Cyc-C düzeylerinin de ileriki çalışmalarda dikkate alınması gerektiği düşünülmektedir.

Anahtar Kelimeler

Biyobelirteçler, Böbrek, Ratlar

Some Renal Marker Levels in Geriatric Rats

Öz

The aim of this study was to determine Neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), cystatin C (Cyc-c), and interleukin-18 (IL-18) levels which are frequently used as kidney biomarkers in geriatric rats and to compare with those in young rats. For this purpose, 12 geriatric Wistar albino rats (30-36 months old) (six males and six females) and 12 (2-3 months old) (six males and six females) Wistar albino rats were used in this study. 24-hour urine samples of all groups were collected, and blood was collected for biochemical analysis under anesthesia. The mean body weight of the geriatric rats was statistically higher than the young rats (p<0.001). The 24-hour urine volume was also statistically higher in geriatric rats than in young ones (p<0.01). Serum Cyc-c (p<0.05), KIM-1 (p<0.01), glucose (p<0.05), total protein (TP) (p<0.001) and creatinine (Crea) levels in geriatric rats (p<0.001) were statistically higher than the young rats. However, there was no statistically significant difference in IL-18, NGAL, and urea (mg/dL) levels. In the 24-hour urine sample, although Cyc-c (p<0.05), Urea (p<0.01), Crea (p<0.01) and protein (p<0.001) levels in geriatric rats were statistically significant compared to young rats; there was no statistically significant difference in KIM-1, IL-18, and NGAL levels. As a result, in geriatric rats, the diagnosis, treatment, and prognosis must be evaluated by considering Crea, glucose, urea, TP levels, and KIM-1, Cyc-C changes. It was concluded that blood Crea, glucose, urea, TP levels as well as KIM-1, Cyc-C levels, and urinary Cyc-C levels should also be considered in future studies.

Anahtar Kelimeler

Biomarkers, Kidney, Rats

Kaynakça

• Alwan H, Al-Saeed MH (2023). Silver Nanoparticles Biofabricated from Cinnamomum zeylanicum Reduce IL-6, IL-18, and TNF-ɑ in Female Rats with Polycystic Ovarian Syndrome. Int J Fertil Steril, 17 (1), 80-84.
• Andreollo NA, Santos EFD, Araújo MR, Lopes LR (2012). Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig, 25 (1), 49-51.
• Ashworth CT, Erdmann R R, Arnold NJ (1960). Age changes in the renal basement membrane in rats. Am J Pathol, 36 (2), 165.
• Aydemir T, Çetin Ş (2019). Chronic Diseases and Psychosocial Care. Jamer, 4 (3), 109-115.
• Basbugan Y, Ağaoğlu ZT (2018). Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. Van Vet J, 29 (3), 135-141.
• Bendayan M (1998). Immunocytochemical detection of advanced glycated end products in rat renal tissue as a function of age and diabetes. Kidney Int, 54 (2), 438-447.
• Çapar E, Çapar A (2018). Geriatric Problems in Elderly Dialysis Patients. Jogs, 1 (3), 119-125.
• Devireddy LR, Gazin C, Zhu X, Green MR (2005). A cell-surface receptor for lipocalin 24p3 selectively mediates apoptosis and iron uptake. Cell, 123 (7), 1293-1305.
• Dirik D, Kömüroğlu AU, Koşal V et al. (2021). Protective Effects of Chrysin in Rats with Ovarian Torsion. Kafkas Univ Vet Fak Derg, 27 (4), 417-424.
• Döventaş A, Döventaş YE (2012). Yaşlılarda Laboratuvar Testlerinin Yorumlanması. Klinik Gelişim, 25 (3), 9-12.
• Duymaz G, Yağar S, Özgök A (2017). Comparison of Effects of Low-Flow Sevoflurane and Low-Flow Desflurane Anaesthesia on Renal Functions Using Cystatin C. Turk J Anaesthesiol Reanim, 45 (2), 93-97.
• Faubel S, Lewis EC, Reznikov L et al. (2007). Cisplatin-induced acute renal failure is associated with an increase in the cytokines interleukin (IL)-1β, IL-18, IL-6, and neutrophil infiltration in the kidney. J Pharmacol Exp Ther, 322 (1), 8-15.
• Gereklioğlu Ç, Başhan İ, Poçan G, Akpınar E (2007). Geriatrik Popülasyonda Anemi: Doğal Süreç mi? AKTD, 16 (1), 70-81.
• Grubb A (1992). Diagnostic value of analysis of cystatin C and protein HC in biological fluids. Clinical Nephrol, 38 (1), 20-27.
• Hagiwara M, Ishiyama S, Mochizuki K (2023). Medium-chain triglycerides reduce glomerulosclerosis and induce expression of redox genes in NSY mice with diabetic nephropathy. Pharma Nutrition, 23, 100325, 1-7.
• Ham RJ (2007). Primary care geriatrics: a case-based approach. 4. Edition. Elsevier Health Sciences, USA.
• Han WK, Bailly V, Abichandani R, Thadhani R, Bonventre JV (2002). Kidney Injury Molecule-1 (KIM-1): a novel biomarker for human renal proximal tubule injury. Kidney Int, 62 (1), 237-244.
• Huang CQ, Ma GZ, Tao MD et al. (2009). The relationship among renal injury, changed activity of renal 1-α hydroxylase and bone loss in elderly rats with insulin resistance or Type 2 diabetes mellitus. J Endocrinol Invest, 32 (3), 196-201.
• Huang J, Caliskan Guzelce E, Gholami SK et al. (2023). Effects of Mineralocorticoid Receptor Blockade and Statins on Kidney Injury Marker 1 (KIM-1) in Female Rats Receiving L-NAME and Angiotensin II. Int J Mol Sci, 24 (7), 6500-6519.
• Ichimura T, Bonventre JV, Bailly V et al. (1998). Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J Biol Chem, 273 (7), 4135-4142.
• İde T (2003). Laboratuvar Hayvanları Biliminin Temel İlkeleri. Medipres Matbaacılık, Ankara.
• Jerkić M, Vojvodić S, López-Novoa JM (2001). The mechanism of increased renal susceptibility to toxic substances in the elderly Part I: The role of increased vasoconstriction. Int Urol Nephrol, 32 (4), 539-547.
• Kumsar AK, Yılmaz FT (2014). Overview of Quality of Life in Chronic Disease Patients. ERÜ Sağlık Bilimleri Fakültesi Dergisi, 2 (2), 62-70.
• Kuwabara T, Mori K, Mukoyama M et al. (2009). Urinary neutrophil gelatinase-associated lipocalin levels reflect damage to glomeruli, proximal tubules, and distal nephrons. Kidney Int, 75 (3), 285-294.
• Laterza OF, Price CP, Scott MG (2002). Cystatin C: an improved estimator of glomerular filtration rate? Clinical Chem, 48 (5), 699-707.
• Ledoux D, Monchi M, Chapelle JP, Damas P (2007). Cystatin C blood level as a risk factor for death after heart surgery. Euro Heart J, 28 (15), 1848-1853.
• Liangos O, Perianayagam MC, Vaidya VS et al. (2007). Urinary N-acetyl-beta-(D)-glucosaminidase activity and kidney injury of urinary liver type fatty acid binding molecule-1 level are associated with adverse outcomes in acute renal failure. J Am Soc Nephrol, 18 (3), 904–912.
• Mori K, Lee HT, Rapoport D et al. (2005). Endocytic delivery of lipocalin-siderophore-iron complex rescues the kidney from ischemia-reperfusion injury. J Clin Invest, 115 (3), 610-621.
• Özbek Z, Öner P, Biyokimya Laboratuvarı İ (2008). Biochemical and Physiological Changes of Aging. Türk Klinik Biyokimya Derg, 6 (2), 73-80.
• Özcan M, Kapucu S (2014). Üriner inkontinansı olan geriatrik hastalara hemşirelik yaklaşımı. Hacet Üniv Hemşire Fak Derg, 1 (2), 101-109.
• Parikh CR, Abraham E, Ancukiewicz M, Edelstein CL (2005). Urine IL-18 is an early diagnostic marker for acute kidney injury and predicts mortality in the intensive care unit. J Am Soc Nephrol, 16 (10), 3046–3052.
• Paul A, Newbigging NS, Lenin A et al. (2023). Role of neutrophil gelatinase-associated lipocalin (NGAL) and other clinical parameters as predictors of bacterial sepsis in patients presenting to the emergency department with fever. Indian J. Crit. Care Med: Peer-reviewed, Official Publication of Indian Society of Crit. Care Med, 27 (3), 176-182.
• Quinn R (2005). Comparing rat's to human's age: how old is my rat in people years? Nutrition, 21 (6), 775-777. Rosenthal RA, Kavic SM (2004). Assessment and management of the geriatric patient. Crit Care Med, 32 (4), 92-105.
• Sengupta PA (2011). Scientific Review of Age Determination for a Laboratory Rat: How old is it in comparison with Human age? Biomed Int, 2 (2), 81–89.
• Sorva A (1992). Correction of serum calcium values for albumin biased in geriatric patients. Arch Gerontol Geriatr, 15 (1), 59-69.
• Vaidya VS, Ford GM, Waikar SS et al. (2009). A rapid urine test for early detection of kidney injury. Kidney Int, 76 (1), 108-114.
• Westhuyzen J (2006). Cystatin C: a promising marker and predictor of impaired renal function. Ann Clin Lab Sci, 36 (4), 387-394.
• Xing Z, Gong K, Hu N, Chen Y (2023). The Reduction of Uromodulin, Complement Factor H, and Their Interaction Is Associated with Acute Kidney Injury to Chronic Kidney Disease Transition in a Four-Time Cisplatin-Injected Rat Model. Int J Mol Sci, 24 (7), 6636-6650.
• Yang J, Mori K, Li JY, Barasch J (2003). Iron, lipocalin, and kidney epithelia. Am J Physiol Renal Physiol, 285 (1), 9-18.
• Zhang Z, Lu B, Sheng X, Jin N (2011). CyC in prediction of acute kidney injury: a systemic review and meta-analysis. Am J Kidney Dis, 58 (3), 356-365.