Farklı Şekillerde Hazırlanarak Değişik Sürelerde Depolanan Liyofilize Edilmiş ve Dondurulmuş Doğal Laktik Asit Bakteri Sıvılarının Yonca Silaji Kalitesi Üzerine Etkisi

Öz

Bu çalışma kapsamında, önceki çalışmadan elde edilen sonuçlarına göre farklı seviyelerde sükroz ilavesi (%5-10) ve farklı sürelerde inkübasyonla (2 ve 5 gün) hazırlanmış fermente edilmiş laktik asit bakteri (LAB) sıvılarının derin dondurucuda dondurularak ve liyofilizasyon işlemi ile kurutularak bir ve üç ay süre ile depolanması sonucunda belirlenen en yüksek LAB sayılarına sahip grupların yonca silajı kalitesi üzerine etkisi belirlenmesi amaçlanmıştır. Çalışmada gruplar kontrol, 2D5%STsL (%5 sükroz ilavesi ile 2 gün inkübasyon sonrasında TRİS (Ts) katkılı liyofilize), 2D10%SDsL (%10 sükroz ilavesi ile 2 gün inkübasyon sonrasında DMSO (Ds) katkılı liyofilize), 5D10%SDsL (%10 sükroz ilavesi ile 5 gün inkübasyon sonrasında DMSO katkılı liyofilize) ve 5D5%STsD'den (%5 sükroz ilavesi ile 5 gün inkübasyon sonrasında TRİS katkılı derin dondurucu) oluşuyordu.Çalışmada bir aylık depolama süresi sonunda LAB sayısı, CO2, laktik asit (LA) içeriği, asetik asit (AA) içeriği, pH, NH3-N/TN ve bütirik asit (BA) değerleri gruplar arasında istatistiksel olarak anlamlı bulunmuştur. Çalışmada üç aylık depolama süresi sonunda ise gruplar arasında ham protein (CP), pH, LA ve BA değerleri istatistiksel olarak anlamlı bulunmuştur.

Anahtar Kelimeler

• Probiyotik
• Silaj
• Yonca

The Effect of Lyophilized and Frozen Natural Lactic Acid Bacteria on Alfalfa Silage Quality Prepared in Different Ways and Stored for Different Periods of Time

Öz

Within the scope of this study, it was aimed to determine the effect of the groups with the highest LAB numbers determined as a result of storage for one and three months on alfalfa silage quality by freezing fermented lactic acid bacteria (LAB) liquids prepared with different levels of sucrose addition (5-10%) and incubation (2 and 5 days) for different periods of time in deep freezer and by drying via lyophilization process according to the results obtained from the previous study.In the study, groups consisted of control, 2D5%STsL (lyophilized with TRIS (Ts) after 2 days incubation with 5% sucrose addition), 2D10%SDsL (lyophilized with DMSO (Ds) after 2 days incubation with 10% sucrose addition), 5D10%SDsL (DMSO (Ds) additive lyophilized after 5 days of incubation with 10% sucrose addition), and 5D5%STsD (TRIS (Ts) additive deep freezer after 5 days of incubation with 5% sucrose addition).In the study, LAB count, CO2, lactic acid (LA) content, acetic acid (AA) content, pH, NH3-N/TN, and butyric acid (BA) values were statistically significant between the groups at the end of the one-month storage period. Crude protein (CP), pH, LA, and BA values were found to be statistically significant between the groups at the end of the three-month storage period in the study.

Anahtar Kelimeler

• Alfalfa
• Probiotics
• Silage

Kaynakça

1. Ammann P, Pfisterer M, Fehr T, Rickli H (2004). Raised cardiac troponins. Br Med J, 328, 1028-1029.
2. Anas AA, Wiersinga WJ, de Vos AF, van der Poll T (2010). Recent insights into the pathogenesis of bacterial sepsis. Neth J Med, 68, 147-152.
3. AOAC (1990). Official Method of Analysis. XV. Edition. Association of Official Analytical Chemistry, Washington.
4. AOAC (2005). Official Methods of Analysis. XVIII Edition. Association of Official Analytical Chemiststry, Arlington.
5. Ashbell G, Weinberg ZG, Azrieli A, Hen Y, Horev, B (1991). A simple system to study the aerobic determination of silages. Can Agric Eng, 34, 171-175.
6. Aydın SS (2014). Farklı Sukroz Seviyeleri ve İnkubasyon Sürelerinde Hazırlanan Fermente Edilmiş Doğal Laktik Asit Sıvısının, Laktik Asit Bakterileriile Yonca Silajı Kalitesine Etkisi. Yüksek Lisans tezi, Harran Üniversitesi, Sağlık Bilimleri Enstitüsü, Şanlıurfa, Türkiye.
7. Bai J, Xu D, Xie D at al. (2020). Effects of antibacterial peptide-producing Bacillus subtilis and Lactobacillus buchneri on fermentation, aerobic stability, and microbial community of alfalfa silage. Bioresour Technol, 315, e123881.
8. Carpintero CM, Henderson AR, McDonald P (1979). The effect of some pre‐treatments on proteolysis during the ensiling of herbage. Grass Forage Sci, 34 (4), 311-315.

9. Danner H, Holzer M, Mayrhuber E, Braun R (2003). Acetic acid increases stability of silage under aerobic conditions. Appl Environ Microbiol, 69 (1), 562-567.
10. Davies DR, Merry RJ, Williams AP et al. (1998). Proteolysis during ensilage of forages varying in soluble sugar content. J Dairy Sci, 81 (2), 444-453.
11. Drouin P, Tremblay J, da Silva ÉB, Apper E (2022). Changes to the microbiome of alfalfa during the growing season and after ensiling with Lentilacto bacillus buchneri and Lentilacto bacillus hilgardii inoculant. J of Appl Microbiol, 133 (4), 2331-2347.
12. DuBois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956). Colorimetric method for determination of sugars and related substances. Anal Chem, 28 (3), 350-356.
13. Guo L, Yao D, Li Det al. (2020). Effects of lactic acid bacteria isolated from rumen fluid and feces of dairy cows on fermentation quality, microbial community, and in vitro digestibility of alfalfa silage. Front Microbiol, 10 (2998), 1-11.
14. Hu Z, Niu H, Tong Q et al. (2020). The microbiota dynamics of alfalfa silage during ensiling and after air exposure, and the metabolomics after air exposure are affected by Lactobacillus casei and cellulase addition. Front Microbiol, 11 (519121), 1-17.
15. Huo W, Wang X, Wei Z et al. (2021). Effect of lactic acid bacteria on the ensiling characteristics and in vitro ruminal fermentation parameters of alfalfa silage. Ital J Anim Sci, 20 (1), 623-631.
16. Huo W, Zhang Y, Zhang L et al. (2022). Effect of lactobacilli inoculation on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage. Front Microbiol, 13 (1070175), 1-9.
17. Keleş G (2009). Homofermantatif ve Heterofermantatif Laktik Asit Bakterilerinin Mısır Silajının Kimyasal Kompozisyonu ile Konya Merinosu Toklularda Performansa Etkileri. Doktora Tezi, Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Konya, Türkiye.
18. Kendall NVG (1978). Anormal silages and silage related disease problems. Literature Review on Fermantation of Silage-A Review. In: Grants-In-Aid Committee. National Feed Ingredients Association. West Des Moines, lowa.
19. Kung JrL, Ranjit NK (2001). The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J Dairy Sci, 84 (5), 1149-1155.
20. Kung L, Shaver R (2001). Interpretation and use of silage fermentation analysis reports. Focus on Forage, 3 (13), 1-5.
21. Li R, Jiang D, Zheng M et al. (2020). Microbial community dynamics during alfalfa silage with or without clostridial fermentation. Sci Rep, 10 (1), 1-14.
22. Li Z, Li F, Xie D et al. (2022). Effects of Bacteriocin-Producing Lactiplantibacillus Plantarum on Fermentation, Dynamics of Bacterial Community, and Their Functional Shifts of Alfalfa Silage with Different Dry Matters. Ferment, 8 (12), 690.
23. Lin C, Bolsen KK, Brent BE, Fung DYC (1992). Epiphytic lactic acid bacteria succession during the pre‐ensiling and ensiling periods of alfalfa and maize. J Appl Bacteriol, 73 (5), 375-387.
24. Lindgren S, Pettersson K, Kaspersson A, Jonsson A, Lingvall P (1985). Microbial dynamics during aerobic deterioration of silages. J Sci Food Agric, 36 (9), 765-774.
25. Mcdonald P (1981). The Biochemistry of Silage. Edition, John Wiley & Sons. New York, United States.
26. McDonald P, Edwards RA, Greenhalgh JFD et al. (2002). Animal Nutrition. VI. Edition. Longman Scientific & Technical, Essex, Longman Scientific & Technical, Essex.
27. Muck RE (1996). Inoculation of silage and its effects on silage quality. In: Informational Conference with Dairy and Forage Industries. United States.
28. Na N, Qili M, Wu N et al. (2022). Bacterial community and fermentation quality of ensiling alfalfa with commercial lactic acid bacterial additives. Front Microbiol, 789 (836899), 1-11.
29. Nsereko VL, Smiley BK, Rutherford WM et al. (2008). Influence of inoculating forage with lactic acid bacterial strains that produce ferulate esterase on ensilage and ruminal degradation of fiber. Anim Feed Sci Technol, 145 (1-4), 122-135.
30. Pahlow G, Honig H (1986).Wirkungsweise Und Einsatzgrenzen Von Silage Impfkulturen Aus Milchsäurebakterien. Das Wirtschaftseigene Futter, 32 (1), 20-35.
31. Playne MJ, McDonald P (1966). The buffering constituents of herbage and of silage. J Sci Food Agric, 17 (6), 264-268.
32. Polan CE, Stieve DE, Garrett JL (1998). Protein preservation and ruminal degradation of ensiled forage treated with heat, formic acid, ammonia, or microbial inoculant. J Dairy Sci, 81 (3), 765-776.
33. Spoelstra SF, Hindle VA (1989). Influence of wilting on chemical and microbial parameters of grass relevant to ensiling. Neth J Agri Sci, 37 (4), 355-364.
34. Sun L, Jiang Y, Ling Q et al. (2021). Effects of adding pre-fermented fluid prepared from red clover or Lucerne on fermentation quality and in vitro digestibility of red clover and Lucerne silages. Agriculture, 11 (5), 454.
35. Suzuki M, Lund C W (1980). Improved gas-liquid chromatography for simultaneous determination of volatile fatty acids and lactic acid in silage. J Agric Food Chem, 28 (5), 1040-1041.
36. Taylor CC, Ranjit N J, Mills J A, Neylon J M, Kung Jr L (2002). The effect of treating whole-plant barley with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for dairy cows. J Dairy Sci, 85 (7), 1793-1800.
37. Van Soest PV, Robertson J B, Lewis BA (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci, 74 (10), 3583-3597.
38. Wang Y, Ke W, Lu Q, Zhang G (2023). Effects of Bacillus coagulans and Lactobacillus plantarum on the Fermentation Characteristics, Microbial Community, and Functional Shifts during Alfalfa Silage Fermentation. Anim, 13 (5), 932.
39. Weinberg ZG, Ashbell G, Azrieli A (1988). The effect of applying lactic bacteria at ensilage on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J Appl Bacteriol, 64 (1), 1-7.
40. Yang F, Wang Y, Zhao, S, Wang Y (2020). Lactobacillus plantarum inoculants delay spoilage of high moisture alfalfa silages by regulating bacterial community composition. Front Microbiol, 11 (1989), 1-13.