Effect of Bee Bread (Perga) on Histopathological Changes and Immunohistochemical Expression of Apoptosis Markers in the Kidney of Rats Exposed to Cadmium

Öz

Cadmium (Cd) is an environmental and industrial pollutant that causes toxicity in various organs in humans and animals. Bee bread (perga) is a natural flavonoid with a wide range of pharmacological properties. This study was conducted to examine the effects of perga on Cd-induced nephrotoxicity. Thirty-two male Wistar rats were randomly divided into 4 groups, as the Control group, Cd group (5 mg/kg/day, orally), Perga group (0.5 g/kg/day, orally), and Cd + Perga group. At the end of the 28-day experiment, kidney tissue samples were taken and histopathological, immunohistochemical, and biochemical analyses were performed. Histopathologically, severe tubular and glomerular damage occurred as a result of Cd exposure in the Cd group. Immunohistochemically, there was an increase in caspas-3 and Bax expression in the renal tissue in the Cd group. According to the biochemical results, while the catalase, superoxide dismutase, and glutathione peroxidase antioxidant enzyme levels decreased in the Cd group, and the malondialdehyde levels increased. However, most of the above-mentioned Cd-induced changes were attenuated by treatment with perga in the Perga + Cd group. In conclusion, perga supplementation may alleviate Cd-induced renal injury through inhibition of apoptosis in renal tissue.

Anahtar Kelimeler

• Cadmium
• Histopathology
• Kidney
• Perga
• Rat.

Etik Beyan

The local ethics committee of Van Yüzüncü Yıl University Animal Experiments approved the study (01/06/2023, 2023/07-12).

Arı Ekmeğinin (Perga) Kadmiyuma Maruz Kalan Sıçan Böbreğindeki Histopatolojik Değişiklikler ve Apoptoz Belirteçlerinin İmmünohistokimyasal Ekspresyonu Üzerine Etkisi

Öz

Kadmiyum (Cd), insanlarda ve hayvanlarda çeşitli organlarda toksisiteye neden olan çevresel ve endüstriyel bir kirleticidir. Arı ekmeği (perga), çok çeşitli farmakolojik özelliklere sahip doğal bir flavonoiddir. Bu çalışma, perganın Cd kaynaklı nefrotoksisite üzerindeki etkilerini incelemek amacıyla yapıldı. Otuz iki adet erkek Wistar sıçanı kontrol grubu, Cd grubu (5 mg/kg/gün, oral), Perga grubu (0.5 g/kg/gün, oral) ve Cd+Perga grubu olmak üzere rastgele 4 gruba ayrıldı. 28 günlük deneme süresi sonunda böbrek dokusu örnekleri alınarak histopatolojik, immünohistokimyasal ve biyokimyasal analizler yapıldı. Histopatolojik olarak Cd grubunda, Cd maruziyeti sonucu ciddi tübüler ve glomerüler hasar meydana geldi. İmmünohistokimyasal olarak Cd grubunda böbrek dokusunda caspas-3 ve Bax ekspresyonunda artış vardı. Biyokimyasal sonuçlara göre Cd grubunda katalaz, süperoksit dismutaz ve glutatyon peroksidaz antioksidan enzim seviyeleri azalırken, malondialdehit seviyeleri arttı. Ancak yukarıda belirtilen Cd'nin neden olduğu değişikliklerin çoğu, Perga + Cd grubunda perga tedavisiyle azaldı. Sonuç olarak, perga takviyesi böbrek dokusunda apoptozun inhibisyonu yoluyla Cd kaynaklı böbrek hasarını hafifletebilir.

Anahtar Kelimeler

• Böbrek
• Histopatoloj
• Kadmium
• Perga
• Rat

Kaynakça

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