Histopathological and Immunohistochemical Investigation on Effects of Boric Acid Used in Treatment of Rats with Knee Osteoartritis on Kidney Tissues

Yıl 2024, , 145 - 151, 28.11.2024

Kübra Asena Terim Kapakin , İsmail Bolat , Esra Manavoğlu Kirman , Gülşah Gündoğdu , Koksal Gundogdu , Fatma Demirkaya Miloğlu , Seymanur Yılmaz Tascı

https://doi.org/10.36483/vanvetj.1477410

Öz

Osteoarthritis (OA) is a disease that often occurs in the knee joints and is characterized by disruption of cartilage homeostasis. Due to the systemic inflammation it creates, it affects not only the joint area, but also many tissues and organs. In this study, the damage caused by systemic inflammatory reactions due to OA in kidney tissue and the protective effect of boric acid were investigated. Wistar albino rats were used in the study. An experimental knee OA model was created by intraarticular injection of monosodium iodoacetate (MIA) in rats. It was formed from 4 groups as Control, OA, OA + 4 mg Boric Acid, OA + 10 mg Boric Acid to work. At the end of the study, kidney tissues were taken from the rats and TNF-α, IL-1β, NOS2 and MMP13 analyzes were performed by histopathological and immunohistochemical methods. Histopathological examinations revealed severe degenerative and necrotic changes in tubular epithelial cells in the OA groups, and these changes decreased in the boric acid-administered group depending on the dose. In immunohistochemical analyzes, it was determined that systemic inflammatory reactions occurring in OA application decreased in a dose-dependent manner with boric acid application. In conclusion; It was determined that kidney tissues were damaged due to systemic inflammatory reactions in rats with OA and boric acid had a protective effect against this damage.

Anahtar Kelimeler

Boric acid, Histopathology, Immunohistochemistry, Kidney, Osteoarthritis

Diz Osteoartritli Sıçanların Tedavisinde Kullanılan Borik Asitin Böbrek Dokuları Üzerindeki Etkilerinin Histopatolojik ve İmmünohistokimyasal Olarak Araştırılması

Öz

Osteoartrit (OA) sıklıkla diz eklemlerinde ortaya çıkan ve kıkırdak homeostazının bozulması ile karakterize bir hastalıktır. Yarattığı sistemik enflamasyon nedeniyle sadece eklem bölgesini değil, birçok doku ve organı da etkilemektedir. Bu çalışmada, OA'ya bağlı sistemik enflamatuar reaksiyonların böbrek dokusunda oluşturduğu hasar ve borik asidin koruyucu etkisi araştırılmıştır. Çalışmada Wistar albino sıçanlar kullanıldı. Sıçanlarda monosodyum iyodoasetatın (MIA) intraartiküler enjeksiyonu ile deneysel diz OA modeli oluşturuldu. Çalışma; Kontrol, OA, OA + 4 mg Borik Asit, OA + 10 mg Borik Asit olmak üzere 4 gruptan oluşturuldu. Çalışma sonunda sıçanlardan böbrek dokuları alınarak histopatolojik ve immünohistokimyasal yöntemlerle TNF-α, IL-1β, NOS2 ve MMP13 analizleri yapıldı. Histopatolojik incelemelerde OA gruplarında tübüler epitel hücrelerinde şiddetli dejeneratif ve nekrotik değişiklikler görülmüş, bu değişiklikler borik asit uygulanan grupta doza bağlı olarak azalmıştır. İmmünohistokimyasal analizlerde ise OA uygulamasında ortaya çıkan sistemik enflamatuar reaksiyonların borik asit uygulaması ile doza bağımlı bir şekilde azaldığı tespit edildi. Sonuç olarak; OA'li sıçanlarda sistemik enflamatuar reaksiyonlar nedeniyle böbrek dokularının hasar gördüğü ve borik asidin bu hasara karşı koruyucu etkisi olduğu tespit edildi.

Anahtar Kelimeler

Borik asit, Histopatoloji, İmmünohistokimya, Böbrek, Osteoartrit.

Kaynakça

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