Research on Hematology, Inflammatory and Antimicrobial Peptide Levels according to Clinical Scoring in Calves with Bovine Respiratory Disease (BRD)

Öz

Bovine respiratory disease (BRD) is a significant and costly disease in cattle, characterized by various infections with distinct causes and clinical signs. This study focuses on investigating some hematological and inflammatory parameters, as well as cathelicidin antimicrobial peptide (CAMP) parameters, in calves with BRD. Forty-two calves were allocated to three groups based on clinical scoring: Group I (n=10, healthy, score 0), Group II (n=16, BRD, score 3), and Group III (n=16, BRD, score 4). Some hematological (WBC, NEU, LYM, NLR, and PLT), inflammatory (IL-1β, TNF-α, NF-ĸB, and IL-10), and CAMP parameters were evaluated in all groups. Group III had significantly higher WBC, NEU, and NLR concentrations than Group I, while concentrations of PLT in Group II were higher than Group I (p<0.05). NF-ĸB, TNF-α, and CAMP levels were enhanced in Group III compared to Group I, and CAMP levels were higher in Group II than in Group I p<0.05). Strong positive correlations were found between NEU and WBC and NLR. Weak positive correlations existed between WBC, NEU, IL-10, and CAMP, as well as between LYM and IL-1β and NF-ĸB and CAMP. In conclusion, the most severe inflammation was observed in Group III, aligning with clinical scoring in BRD-affected calves. CAMP was identified as a reliable marker for inflammation assessment. Additionally, NLR, being low-cost and easily measurable, showed promise as an inflammation indicator.

Anahtar Kelimeler

• Anti-inflammatory
• Bovine Respiratory Disease
• Calf
• Cathelicidin antimicrobial peptide
• Neutrophil-Lymphocyte Ratio
• Pro-inflammatory.

Sığır Solunum Yolu Hastalıklı (BRD) Buzağılarda Klinik Skorlamaya Göre Hematoloji, İnflamatuar ve Antimikrobiyal Peptid Düzeylerinin Araştırılması

Öz

Sığır solunum yolu hastalığı (BRD), sığırlarda farklı nedenleri ve klinik belirtileri olan çeşitli enfeksiyonlarla karakterize önemli ve maliyetli bir hastalıktır. Bu çalışma, BRD'li buzağılarda bazı hematolojik ve inflamatuvar parametrelerin yanısıra katelisidin antimikrobiyal peptid (CAMP) parametrelerinin araştırılmasına odaklanmıştır. 42 buzağı klinik skorlamaya gore üç gruba ayrılmıştır: Grup I (n=10, sağlıklı, skor 0), Grup II (n=16, BRD, skor 3) ve Grup III (n=16, BRD, skor 4). Bazı hematolojik (WBC, NEU, LYM, NLR ve PLT), inflamatuvar (IL-1β, TNF-α, NF-ĸBve IL-10) ve CAMP parametreleri değerlendirilmiştir. Grup III'te WBC, NEU ve NLR konsantrasyonları Grup I'e göre istatistiksel olarak yüksekken, Grup II'de PLT konsantrasyonları Grup I'e göre istatistiksel olarak daha yüksekti (p<0.05). NF-ĸB, TNF-α ve CAMP düzeyleri Grup III'te Grup I'e kıyasla artarken, CAMP düzeyleri Grup II'de Grup I'e kıyasla daha yüksekti (p<0.05). NEU ile WBC ve NLR arasında güçlü pozitif korelasyonlar bulundu. WBC, NEU, IL-10 ve CAMP arasında ve ayrıca LYM ile IL-1β ve NF-ĸB ile CAMP arasında zayıf pozitif korelasyonlar mevcuttu. Sonuç olarak, BRD'den etkilenen buzağılarda klinik skorlama ile uyumlu olarak en şiddetli inflamasyon Grup III'te gözlenmiştir. CAMP, inflamasyon değerlendirmesi için güvenilir bir belirteç olarak tanımlanmıştır. Ayrıca, düşük maliyetli ve kolay ölçülebilir NLR, bir yangı göstergesi olarak umut vaat etmektedir.

Anahtar Kelimeler

• Anti-inflamatuar
• Sığır Solunum Hastalığı
• Buzağı
• Cathelicidin antimikrobiyal peptid
• Nötrofil-Lenfosit Oranı
• Pro-inflamatuar.

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