Ankara’nın Polatlı Yöresindeki Keçilerde Neospora caninum’un Seroprevelansı

Öz

Bu çalışmada Ankara ili Polatlı ilçesinde yetiştirilen keçilerde apicomplexan bir protozoon parazit olan Neospora caninum’un seroprevalansının araştırılması amaçlanmıştır. Bu amaçla Polatlı ilçesinde yetiştirilen 150 keçinin vena jugularisinden usulüne uygun şekilde kan örnekleri toplanmıştır. Toplanan kan örneklerinden elde edilen serumlar ticari ELISA kiti kullanılarak analiz edilmiştir. Yapılan serolojik analizler sonucunda, incelenen 150 kan serumu örneğinin 9’unda (%6) N. caninum varlığı yönünden seropozitiflik tespit edilmiştir. Seropozitiflik belirlenen örneklerin tamamının bir yaş ve üzeri dişi keçilere ait olduğu saptanmıştır. Irklara göre değerlendirildiğinde, seropozitiflik oranı Ankara keçilerinde %8.3 (n=7), Kıl keçilerinde ise %3 (n=2) olarak belirlenmiştir. Ancak elde edilen veriler doğrultusunda ırklar arasında N. caninum seropozitifliği bakımından istatistiksel olarak anlamlı bir fark bulunmamıştır (p>0.05). Yaş ve cinsiyete göre karşılaştırıldığında ise N. caninum seropozitifliği açısından istatistiki olarak anlamlı bir fark bulunmuştur (p<0.05). Bu çalışmada Ankara’nın Polatlı yöresindeki keçilerde N. caninum’un varlığı serolojik olarak ilk kez belirlenmiştir. Yörede ortaya çıkacak olan keçi abortlarında N. caninum’un da göz önüne alınması, bu parazite yönelik koruma ve kontrol tedbirlerinin arttırılması gerektiği düşünülmektedir.

Anahtar Kelimeler

• ELISA
• Keçi
• Neospora caninum
• Seroprevalans.

Proje Numarası

2020/095

The Seroprevalence of Neospora caninum in Goats in Polatlı Region of Ankara

Öz

The aim of this study was to investigate the seroprevalence of N. caninum, an apicomplexan protozoan parasite, in goats raised in Polatlı district of Ankara province. For this purpose, blood samples were collected from the jugular vein of 150 goats in Polatlı district. The seras obtained from the collected blood samples were analysed using a commercial ELISA kit. As a result of serological analyses 9 out of 150 blood serum samples examined (6%) were found to be seropositivity for the presence of N. caninum. It was determined that all of the seropositivity samples belonged to female goats aged one year and above. When evaluated according to breeds, the positivity rate was determined as 8.3% (n=7) in Angora goats and 3% (n=2) in Hair goats. However, according to the obtained data, no statistically significant difference was found between breeds in terms of N. caninum seropositivity (p>0.05). When compared according to age and gender, a statistically significant difference was found in terms of N. caninum seropositivity (p<0.05). In this study, N. caninum was detected serologically for the first time in goats of Polatlı region of Ankara. It is thought that N. caninum should be taken into consideration in goat abortions that may occur in the region and that protection and control measures against this parasite should be increased.

Anahtar Kelimeler

• ELISA
• Goat
• Neospora caninum
• Seroprevalence.

Proje Numarası

2020/095

Kaynakça

1. Abo-Shehada MN, Abu-Halaweh MM (2010). Flock-level seroprevalence of, and risk factors for, Neospora caninum among sheep and goats in northern Jordan. Prev Vet Med, 93 (1), 25-32.
2. Al-Majali AM, Jawasreh KI, Talafha HA et al. (2008). Neosporosis in sheep ve different breeds of goats from Southern Jordan: prevalance and risk factors analysis. American J of Animal Vet Sci, 3 (2), 47-52.
3. Atelge M, Karatepe M, Yıldırım A (2022). Seroprevalance of Neospora caninum in goats from Korkuteli district of Antalya. Turkiye Parazitol Derg, 46 (3), 180-183.
4. Aydın L (2013). Sığırlarda Görülen Parazit Hastalıkları. İçinde M. A. Özcel (Edt), Veteriner hekimlikte parazit hastalıkları (Volume: 1, pp: 65-70). Türkiye Parazitoloji Derneği Yayını. İzmir.
5. Beki FHK, Azizi H, Kojouri G et al. (2023). Seroprevalence of neosporosis in goats and farm dogs at the central Zagros region (Iran). Iran Vet J, 19 (3), 41-44.
6. Cayvaz M, Karatepe M (2011). Seroprevalence of Neospora caninum in goats in Niğde province. Kafkas Univ Vet Fak Derg, 17 (6), 935-939.
7. Çelik BA, Çelik ÖY, İrak K et al. (2022). Oxidant/Antioxidant status and certain trace elements relationship in hair goats naturally infected by Neospora caninum. J Hellenic Vet Med Soc, 73 (3), 4417-4424.
8. Dubey JP (2003). Review of Neospora caninum and neosporosis in animals. Korean J Parasitol, 1 (41), 1-16.

9. Dubey JP, Aciand HM, Hamir AN (1992). Neospora caninum (Apicomplexa) in stilborn goat. J Parasitol, 78 (3), 532-534.
10. Dubey JP, Buxton D, Wouda W (2006). Pathogenesis of bovine neosporosis. J Comp Pathol, 134, 267–89.
11. Dubey JP, Lindsay DSA (1996). Review of Neospora caninum and neosporosis. Vet Parasitol, 67 (1-2), 1-59.
12. Dubey JP, Schares G (2011). Neosporosis in animals the last five years. Vet Parasitol, 180 (1-2), 90-108.
13. Dumanlı N, Aktaş M (2010). Toxoplasmatidae. In: N. Dumanlı, ve Z. Karaer (Edt), Veteriner Protozooloji (Volume: 1, pp. 119-135). Medisan Yayın Evi, Ankara, Türkiye.
14. Dyer RM, Jenkins MC, Kwok OCH et al. (2000). Serologic survey of Neospora caninum infection in a closed dairy cattle herd in Maryland: risk of serologic reactivity by production groups. Vet Parasitol, 90 (3), 171-181.
15. Eser M, Varol K (2022). Seroprevalence of Neospora caninum and Toxoplasma gondii in Honamlı goats in Burdur province. Kocatepe Vet J, 15 (2), 144-150.
16. Fereig RM, Wareth G, Abdelbaky HH et al. (2022). Seroprevalence of specific antibodies to Toxoplasma gondii, Neospora caninum, and Brucella spp. in sheep and hoats in Egypt. Animals, 12, 3327.
17. Georgieva DA, Prelezov PN, Koinarski VTS (2006). Neospora caninum and neosporosis in animals-A Review. Bulg J Vet Med, 9, 126.
18. Gharekhani J, Esmaeilnejad B, Rezaei H et al. (2016). Prevalence of anti-Neospora caninum antibodies in Iranian goats. Ann Parasitol, 62 (2), 111-114.
19. Ghattof HH, Faraj AA (2015). Seroprevalence of Neospora caninum in goats in Wasit province Iraq. Int J Curr Microbiol Appl Sci, 4 (7), 182-191.
20. Goodswen SJ, Kennedy PJ, Ellis JT (2013). A review of the infection, genetics, and evolution of Neospora caninum: From the past to the present. Infect Genet Evol, 13, 133-150.
21. Gökpınar S (2022). Seroprevalence of Neospora caninum in Bristle goats in Kırıkkale region. In: Proceedings of International Korkut Ata Scientific Researches Conference, Osmaniye, Turkey.
22. Hu XH, Xie SC, Liang QL et al. (2022). Seroprevalence and risk factors of Toxoplasma gondii and Neospora caninum infection in black goats in Yunnan Province, Southwestern China. Front Vet Sci, 9, 975238.
23. King JS, Slapeta J, Jenkins DJ et al. (2010). Australian dingoes are definitive hosts of Neospora caninum. Int J Parasitol, 40 (8), 945-950.
24. McAllister MM (2022). Neosporosis in Animals, MSD Manual Veterinary Manual, https://www.msdvetmanual.com/generalized-conditions/neosporosis/neosporosis-in-animals. McAllister MM, Dubey JP, Lindsay DS et al. (1998). Rapid communication: dogs are definitive hosts of Neospora caninum. Int J Parasitol, 28, 1473-1478.
25. Mesquita LP, Costa RC, Nogueria CI et al. (2018). Placental lesions associated with abortion and stillbirth in goats naturally infected by Neospora caninum. Pesq Vet Bras, 38 (3), 444-449.
26. Moore DP, De Yaniz MG, Odeon AC et al. (2007). Serological evidence of Neospora caninum infections in goats from La Rioja province, Argentina. Small Rumin Res, 73, 256-258.
27. Naguleswaran A, Hemphill A, Rajapakse RPVJ et al. (2004). Elaboration of crude antigen ELISA for serodiagnosis of caprine neosporosis: validation of the test by detection of Neospora caninum-spesific antibodies in goats from Sri Lanka. Vet Parasitol, 126 (3), 257-262.
28. Nasir A, Ashraf M, Khan MS et al. (2012). Prevalence of Neospora caninum antibodies in sheep and goats in Pakistan. J Parasitol, 98 (1), 213-215.
29. Özdamar D, Karatepe B, Yıldırım A (2021). Investigation of anti-Neospora caninum antibodies in goats in Mesudiye district of Ordu using ELISA. Kocatepe Vet J, 14 (1), 1-6.
30. Porto WJN, Regidor-Cerrillo J, Kim PCP et al. (2016). Experimental caprine neosporosis: the influence of gestational stage on the outcome of incection. Vet Res, 47 (1), 29-39.
31. Rinaldi L, Fusco G, Musella V et al. (2005). Neospora caninum in pastured cattle: determination of climatic, environmental, farm management and individual animal risk factors using remote sensing and geographical information systems. Vet Parasitol, 128 (3-4), 219-230.
32. Rodrigues AA, Reis SS, da Silva Moraes E et al. (2021). Seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in goats of Maranh˜ ao State, Brazil. Vet Parasitol: Reg Stud Rep, 26, 100634.
33. Sadiq MB, Muhamad AS, Hamdan SA et al. (2023). Seroprevalence and factors associated with Toxoplasma gondii, Neospora caninum, and Besnoitia besnoiti infections in cattle and goats in Selangor, Malaysia. Animals, 13, 948.
34. Toolan DP (2003). Neospora caninum abortion in cattle a clinical perspective. Irish Vet J, 56, 404-410.
35. Toy A, Oğuz B (2023). Seroprevalence of Neospora caninum in goats in Gevas district of Van. MJAVL, 13 (1), 59-66.
36. Topazio JP, Weber A, Camillo G et al. (2014). Seroprevalence and risk factors for Neospora caninum in goats in Santa Catarina state, Brazil. Braz J Vet Parasitol, 23 (3), 360-366.
37. Udonsom R, Supanta J, Tanglakmankhong O et al. (2021). Toxoplasma gondii and Neospora caninum prevalence and risk factors on goat farms in Kanchanaburi province, Thailand. Vet Integr Sci, 19 (1), 65-74.
38. Utuk AE, Eski F (2017). Detection of anti-Neospora caninum antibodies in a goat flock in Kilis province. Inter J Vet Sci, 6 (2), 114-117.
39. Utuk AE, Eski F (2019). Investigation of anti-Neospora caninum antibodies and disease-related risk factors in goats. Med Weter, 75 (11), 678-683.
40. Utuk AE, Simsek S, Piskin FC et al. (2011). Detection of Neospora caninum IgG antibodies in goats in Elazig, Erzurum and Kırsehir provinces of Turkey. Isr J Vet Med, 66 (4), 157-160.
41. Varaschin MS, Hirsch C, Wouters F et al. (2012). Congenital neosporosis in goats from the state of Minas Gerais, Brazil. Korean J Parasitol, 50 (1), 63-67.
42. Villagra-Blanco R, Wagner H, Dolz G et al. (2017). First report on the seroprevalence of Neospora caninum in goats from the federal state of hesse, Germany. Berl Munch Tierarztl Wochenschr, 130, 517-522.
43. Von Blumröder D, Stambusch R, Labohm R et al. (2006 Potenzielle risikofaktoren für den serologischen nachweis von Neospora-caninum-infektionen in Rinderherden in Rheinland-Pfalz. Tierarztl Prax, 34 (3), 141-147.
44. Yi XL, Yang WH, Zheng HL et al. (2024). Seroprevalence and molecular detection of Toxoplasma gondii and Neospora caninum in beef cattle and goats in Hunan province, China. Parasit Vectors, 17, 195.