Examining the Impact of 4-Nonylphenol on G-361 Malignant Melanoma Cell Proliferation and Apoptosis Activation

Yıl 2025, Cilt: 36 Sayı: 2, 110 - 114, 27.07.2025

Esra Bilici , Cevdet Uğuz , Korhan Altunbaş

https://doi.org/10.36483/vanvetj.1654843

Öz

Nonylphenol (NP) is among the most prevalent endocrine-disrupting compounds found in the environment. Although the carcinogenic properties of endocrine disruptors have been well investigated, their possible anticancer effects remain comparatively underexplored. This study investigated the cytotoxic and pro-apoptotic effects of NP on G-361 human malignant melanoma cell line. The major objective of the stuyd was to assess anticancer effects of the endocrine-disrupting compound NP on G-361 melanoma cells. The MTT assay was used to determine cell viability, and quantitative real-time PCR was performed to analyze the expression levels of apoptosis-associated genes, including Caspase-3, Bcl-2, Bax, P53, P21, and β-actin. The results indicated a significant decrease in cell viability following treatment with 100 μg/ml NP. Notably, NP exerted a dose-dependent cytotoxic effect, suggesting its possible role in the modulation of apoptotic pathways in melanoma cells. These results require further investigation of different pathways by which NP exerts its effects to gain a deeper insight into its molecular mechanism.

Anahtar Kelimeler

P53 , Melanoma , Nonylphenol , Proliferation

Etik Beyan

Afyon Kocatepe University Animal Experiments Local Ethics Committee gave ethics committee permission for this study with the number 49533702/79 and dated 01/07/2019.

Destekleyen Kurum

This research was supported by Afyon Kocatepe University Scientific Research Projects Coordination Office with the project number “19.SAĞ.BİL.13”.

Proje Numarası

“19.SAĞ.BİL.13”

Teşekkür

This study is derived from publicly defended PhD thesis of the Esra BİLİCİ named author.

G-361 Malignant Melanoma Hücrelerinde 4-Nonilfenolün Hücre Proliferasyonu ve Apoptoz Aktivasyonu Üzerindeki Etkisinin Araştırılması

Öz

Nonilfenol (NP), çevrede bulunan en yaygın endokrin bozucu bileşikler arasındadır. Endokrin bozucuların kanserojen özellikleri iyi araştırılmış olsa da olası antikanser etkileri nispeten yeterince araştırılmamıştır. Bu çalışma, NP'nin G-361 insan malign melanom hücre hattı üzerindeki sitotoksik ve proapoptotik etkilerini araştırmıştır. Çalışmanın temel amacı, endokrin bozucu bileşik NP'nin G-361 melanom hücreleri üzerindeki antikanser etkilerini değerlendirmekti. Hücre canlılığını belirlemek için MTT testi kullanıldı ve Caspase-3, Bcl-2, Bax, P53, P21 ve β-actin dahil olmak üzere apoptozla ilişkili genlerin ifade düzeylerini analiz etmek için kantitatif gerçek zamanlı PCR gerçekleştirildi. Sonuçlar, 100 μg/ml NP ile tedaviden sonra hücre canlılığında önemli bir azalma olduğunu gösterdi. Özellikle, NP doz bağımlı bir sitotoksik etki gösterdi ve bu da melanom hücrelerinde apoptotik yolların modülasyonunda olası bir rolü olduğunu düşündürdü. Bu sonuçlar, NP'nin moleküler mekanizmasına daha derin bir bakış açısı kazanmak için etkilerini gösterdiği farklı yolların daha fazla araştırılmasını gerektiriyor.

Anahtar Kelimeler

P53 , Melanoma , Nonilfenol , Proliferasyon

Proje Numarası

“19.SAĞ.BİL.13”

Kaynakça

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