Investigation of the Effect of Borago officinalis on Bax/Bcl-2/Caspase-3 Pathways Against Spleen Toxicity Induced by Lead Acetate in Rats

Öz

Lead is a highly toxic heavy metal and an environmental pollutant. Lead exposure causes damage and dysfunctions in organs. In tissues exposed to lead, ROS increase, resulting in oxidative stress, inflammation and apoptosis. In this study, we aimed to investigate the protective effects of Borago officinalis (BO) against lead, whose toxic effects are well-established. In this study, 40 Sprague-Dawley rats were used. The rats were randomly divided into 5 groups. The groups were determined as control, BO100, Pb (20 mg/kg), Pb+BO50 and Pb+BO100. After the experimental procedures were completed, the tissues were transported in cold chain and stored in -80 deep freezer until the experiments were performed. Keap-1 level was determined in spleen tissue using Western blot method. At the same time, IL-1β, IL-10, NF-ĸB, Bax, Bcl-2, Caspase-3 levels were determined by RT-PCR method. BO administration stimulated Keap-1 level in relation to oxidative stress. While BO suppressed IL-1β and NF-ĸB levels, it stimulated IL-10 level, which are anti-inflammatory markers. BO also suppressed Bax and Kaspas-3 levels, while significantly stimulating Bcl-2 level. With these effects, it was observed that BO had anti-oxidant, anti-inflammatory, anti-apoptotic effects in the spleen tissue of rats treated with lead acetate.

Anahtar Kelimeler

• Apoptosis
• Borago officinalis
• Inflammation
• Lead acetate
• Oxidative stress.

Ratlarda Kurşun Asetat ile İndüklenen Dalak Toksisitesine Karşı Borago officinalis'in Bax/Bcl-2/Kaspaz-3 yolakları üzerine Etkisinin İncelenmesi

Öz

Kurşun oldukça toksik bir ağır metal olup ve çevresel bir kirleticidir. Kurşun maruziyeti organlarda hasara ve fonksiyon bozukluklarına neden olmaktadır. Kurşuna maruz kalan dokularda ROS artışı buna bağlı, oksidatif stres, inflamasyon ve apoptosis şekillenmektedir. Biz bu çalışmada etkileri bilinen kurşuna karşı Borago officinalis (BO)’nun koruyucu etkilerini araştırmayı amaçladık. Bu çalışmada, 40 adet Sprague- Dawley cinsi ratlar kullanıldı. Ratlar rastgele 5 gruba ayrıldı. Gruplar kontrol, BO100, Pb (20 mg/kg), Pb+BO50 ve Pb+BO100 olarak belirlendi. Deney uygulamaları tamamlandıktan sonra, dokular soğuk zincirde taşınarak, deneylerin yapılıncaya kadar -80 derin dondurucuda saklandı. Dalak dokusunda Western Blot yöntemi kullanılarak Keap-1 düzeyi belirlendi. Aynı zamanda RT-PCR yöntemi kullanılarak IL-1β, IL-10, NF-ĸB, Bax, Bcl-2, Kaspaz-3 düzeyleri belirlendi. BO uygulaması oksidatif stresle ilgili olarak Keap-1 düzeyini uyardı. BO, IL-1β ve NF-ĸB düzeyini baskılarken, anti-inflamatuar markırlar olan IL-10 düzeyini uyardığı belirlendi. BO aynı zamanda Bax ve Kaspas-3 seviyesini baskılarken, Bcl-2 seviyesini önemli ölçüde uyarmaktadır. BO bu etkileriyle kurşun asetat uygulanan ratların dalak dokusunda anti-oksidan, anti-inflamatuar, anti-apoptotik etkilere sahip olduğu gözlendi.

Anahtar Kelimeler

• Apoptosis
• Borago officinalis
• İnflamasyon
• Kurşun asetat
• Oksidatif stres.

Kaynakça

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