Modulatory Role of Hypericum perforatum on Oxidative Stress and Histopathological Changes in Ethanol-Exposed Rat Ovaries

Yıl 2025, Cilt: 36 Sayı: 3, 169 - 175, 30.11.2025

Gülşah Yıldız Deniz , Esra Laloğlu

https://doi.org/10.36483/vanvetj.1691968

Öz

In this study, the possible protective effect of Hypericum perforatum extract (HPE) against ethanol induced ovarian damage was investigated. Rats were allocated into four groups: Group I; Control, Group II (HPE); Rats treated with 200 mg/kg b.w. Group III (Alcoholic); Rats treated with 2.5 g/kg b.w. Group IV (Alcohol+HPE); Alcoholic rats treated with 200 mg/kg b.w. Thiobarbituric acid reactive substances (TBARS), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), levels were measured in ovarian tissues to determine the antioxidative effects of HPE. The histology of the ovarian was examined using hematoxylin & eosin, periodic acid Schiff, masson trichrome and amyloid staining methods. Mechanistically, the ovary of ethanol treated rats exhibited a significant increase in the levels of TBARS and a significant decrease in the levels of GPx, CAT and SOD. We concluded that HPE has a protective effect on ovarian tissue. Interestingly, oral supplementation of ethanol treated rats with HPE significantly abolished collagen deposition, abrogated oxidative stress, and hence repaired the ovarian architecture nearly to the normal architecture observed in the control rats. Our study implicates that HPE has a protective potential against ethanol induced ovarian injury, which might be strongly associated with the induction of oxidative stress arising from ethanol consumption.

Anahtar Kelimeler

Ethanol , Hypericum perforatum , Ovarian tissue

Etik Beyan

All animal manipulations were performed according to the in conformity with the Institutional Ethical Committee for Animal Care and Use at Atatürk University (protocol number: (54826478-E.1900181803/24.06.2019) and the Guide for the Care and Use of Laboratory Animals.

Hypericum perforatum'un Etanole Maruz Kalan Sıçan Yumurtalıklarında Oksidatif Stres ve Histopatolojik Değişiklikler Üzerindeki Düzenleyici Rolü

Öz

Bu çalışmada, Hypericum perforatum ekstraktının (HPE) etanol kaynaklı over hasarına karşı olası koruyucu etkisi araştırıldı. Sıçanlar dört gruba ayrıldı: Grup I; kontrol, Grup II (HPE); 200 mg/kg vücut ağırlığı ile tedavi edilen sıçanlar, Grup III (Alkollü); 2.5 g/kg vücut ağırlığı ile tedavi edilen sıçanlar, Grup IV (Alkol+HPE); 200 mg/kg vücut ağırlığı ile tedavi edilen alkollü sıçanlar. HPE'nin antioksidan etkilerini belirlemek için over dokularında tiyobarbitürik asit reaktif maddeler (TBARS), katalaz (CAT), glutatyon peroksidaz (GPx) ve süperoksit dismutaz (SOD) düzeyleri ölçüldü. Overin histolojisi hematoksilen & eozin, periyodik asit Schiff, masson trikrom ve amiloid boyama yöntemleri kullanılarak incelendi. Mekanik olarak, etanol ile tedavi edilen sıçanların yumurtalıkları TBARS seviyelerinde önemli bir artış ve GPx, CAT ve SOD seviyelerinde önemli bir azalma gösterdi. HPE'nin yumurtalık dokusu üzerinde koruyucu bir etkiye sahip olduğu sonucuna vardık. İlginç bir şekilde, etanol ile tedavi edilen sıçanlara HPE ile oral takviye, kolajen birikimini önemli ölçüde ortadan kaldırdı, oksidatif stresi ortadan kaldırdı ve dolayısıyla yumurtalık yapısını kontrol sıçanlarında gözlemlenen normal yapıya yakın bir şekilde onardı. Çalışmamız, HPE'nin etanol kaynaklı yumurtalık hasarına karşı koruyucu bir potansiyele sahip olduğunu ve bunun etanol tüketiminden kaynaklanan oksidatif stresin indüklenmesiyle güçlü bir şekilde ilişkili olabileceğini ima etmektedir.

Anahtar Kelimeler

Etanol , Hypericum perforatum , Ovaryum dokusu

Kaynakça

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