Investigation of Biofilm Formation Abilities of Escherichia coli, Klebsiella pneumoniae, and Salmonella enterica Isolates from Broiler Chicken Flocks

Yıl 2025, Cilt: 36 Sayı: 3, 181 - 186, 30.11.2025

Seyyide Sarıçam İnce

https://doi.org/10.36483/vanvetj.1712871

Öz

Bacteria use biofilm formation as a critical survival strategy to increase their resistance to environmental stress factors and sanitation procedures, which means that it also plays a key role in the persistence of foodborne pathogens in poultry environments. This study investigated the biofilm formation abilities of Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), and Salmonella enterica (S. enterica) isolates obtained from broiler chicken flocks. A total of 90 isolates were tested for biofilm formation ability, comprising 30 isolates from each species. Biofilm formation ability of the isolates was quantitatively examined by the microtiter plate test. The analysis indicated that all isolates (100.0%, 90/90) were biofilm producers, of which 95.6% (86/90) and 4.4% (4/90) were weak and moderate biofilm producers, respectively. No isolates were strong biofilm producers. All E. coli isolates (100.0%, 30/30) were weak biofilm producers. The prevalence of weak biofilm producers (93.3%, 28/30) and moderate biofilm producers (6.7%, 2/30) was similar for both K. pneumoniae and S. enterica isolates. The mean optical density of K. pneumoniae isolates (0.094) was higher than those of the E. coli and S. enterica isolates (0.079 and 0.090, respectively). To the best of knowledge, this study is the first study to investigate biofilm formation ability of E. coli and K. pneumoniae isolates originated from broiler chicken flocks in Türkiye. It was revealed a high prevalence of biofilm formation among the analyzed enteric pathogens in broiler chicken flocks. High biofilm-forming potential increases bacterial persistence in poultry environments, thereby complicating sanitation measures and increasing the risk of foodborne transmission. The present study emphasizes the need for improved control strategies against biofilm-forming bacteria in poultry production systems to reduce the threat to public health.

Anahtar Kelimeler

Biofilm , E. coli , K. pneumoniae , Microtiter plate method , Poultry , S. enterica.

Broyler Tavuk Sürülerinden İzole Edilen Escherichia coli, Klebsiella pneumoniae ve Salmonella enterica İzolatlarının Biyofilm Oluşturma Kapasitelerinin Araştırılması

Öz

Bakteriler, çevresel stres faktörlerine ve sanitasyon uygulamalarına karşı dirençlerini artırmak amacıyla biyofilm oluşumunu hayatta kalma stratejisi olarak kullanmaktadır. Bu durum, biyofilm oluşumunun, kanatlı hayvan yetiştiriciliğinde gıda kaynaklı patojenlerin kalıcılığında kritik bir rol oynamasına neden olmaktadır. Bu çalışmada, broyler tavuk sürülerinden elde edilen Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae) ve Salmonella enterica (S. enterica) izolatlarının biyofilm oluşturma kapasiteleri araştırılmıştır. Her türden 30’ar adet olmak üzere toplam 90 adet izolat, biyofilm oluşturma kapasiteleri açısından değerlendirilmiştir. İzolatların biyofilm oluşturma kapasiteleri, mikrotitre plak testi ile kantitatif olarak incelenmiştir. Yapılan analizler, tüm izolatların (%100,0, 90/90) biyofilm oluşturduğunu göstermiştir. Bu izolatların %95,6’sı (86/90) zayıf, %4,4’ü (4/90) ise orta düzeyde biyofilm üreticisi olarak sınıflandırılmıştır. Güçlü biyofilm üreticisi izolat tespit edilmemiştir. Tüm E. coli izolatları (%100,0, 30/30) zayıf biyofilm üreticisi olarak sınıflandırılmıştır. K. pneumoniae ve S. enterica izolatlarında zayıf (%93,3, 28/30) ve orta düzeyde (%6,7, 2/30) biyofilm üreticisi olma oranları benzer bulunmuştur. K. pneumoniae izolatlarının ortalama optik yoğunluğu (0,094), E. coli ve S. enterica izolatlarına (sırasıyla 0,079 ve 0,090) göre daha yüksek bulunmuştur. Bilinen kadarıyla bu çalışma, Türkiye'deki etlik piliç sürülerinden elde edilen E. coli ve K. pneumoniae izolatlarının biyofilm oluşturma kapasitesini araştıran ilk çalışmadır. Broyler tavuk sürülerinde analiz edilen enterik patojenler arasında biyofilm oluşumunun yüksek yaygınlıkta olduğu ortaya konmuştur. Yüksek biyofilm oluşturma potansiyeli, bakterilerin kümes ortamlarında kalıcılığını artırarak hijyen uygulamalarını zorlaştırmakta ve gıda kaynaklı bulaşma riskini artırmaktadır. Bu çalışma, halk sağlığına yönelik tehditlerin azaltılması amacıyla, kanatlı üretim sistemlerinde biyofilm oluşturan bakterileri hedefleyen gelişmiş kontrol stratejilerinin geliştirilmesi gerektiğini göstermektedir.

Anahtar Kelimeler

Biyofilm , E. coli , Kanatlı , K. pneumoniae , Mikrotitre plak yöntemi

Kaynakça

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