Comparison of CLIA and ELFA Progesterone Assay Methods for Evaluating Pregnancy in Kilis Goats

Öz

Early pregnancy diagnosis is needed for small ruminants. Effective methods for early pregnancy detection are of great importance. Identifying pregnancy enhances rebreeding and is crucial for controlled breeding programs. However, various methods exist for diagnosing pregnancy in small ruminants. The choice of methods is influenced by equipment availability, the number of days post-insemination and the required accuracy level. Measurement of progesterone concentrations is used in pregnancy diagnosis in small ruminants. The detection of progesterone (P4) assays can facilitate the diagnosis of pregnancy approximately three weeks post-insemination. This study aimed to evaluate and compare the accuracy of two progesterone assays (CLIA and ELFA) for the early diagnosis of pregnancy (at day 21 post-insemination) in Kilis goats, using transrectal ultrasonography at day 50 as the reference standard. Kilis goats (n: 150) were synchronized with a 10-day intravaginal sponge. Artificial insemination applications were intracervically performed with frozen-thawed semen straws. Blood samples were collected 21 days post-insemination to assess progesterone levels for pregnancy diagnosis. The pregnancy rate and average progesterone level determined by the CLIA method were found to be higher than those determined by the ELFA method. Additionally, in early pregnancy determination, the number of truly pregnant animals was significantly higher, with sensitivity, specificity, and accuracy. The CLIA method is a precise and dependable approach for measuring plasma progesterone levels. In the study, it was concluded that the CLIA progesterone assay method evaluated for early pregnancy detection in Kilis goats was superior.

Anahtar Kelimeler

• CLIA
• ELFA
• Goat
• Pregnancy
• Progesterone levels

Etik Beyan

The Hatay Mustafa Kemal University Scientific Ethical Committee approved the experiments (2022/08-01).

Kilis Keçilerinde Gebeliğin Değerlendirilmesinde CLIA ve ELFA Progesteron Analiz Yöntemlerinin Karşılaştırılması

Öz

Küçük ruminantlarda erken gebelik tanısı gereklidir. Gebeliğin erken tespiti için etkili yöntemler büyük önem taşır. Gebeliğin belirlenmesi yeniden üremeyi artırır ve kontrollü üreme programları için çok önemlidir. Küçük ruminantlarda gebeliğin teşhisi için çeşitli yöntemler mevcut olsa da yöntem seçimi ekipman mevcudiyetine üreme sonrası gün sayısına ve gereken doğruluk düzeyine bağlıdır. Küçük ruminantlarda gebelik teşhisinde progesteron konsantrasyonlarının ölçümü kullanılır. Progesteron (P4) analizlerinin tespiti üreme sonrası yaklaşık üç hafta içinde gebelik teşhisini kolaylaştırabilir. Bu makale, CLIA ve ELFA yöntemleri ile gebelik teşhisinin hassasiyetini değerlendirmeyi önermektedir. Kilis keçileri (n:150) 10 günlük intravajinal süngerle senkronize edildi. Suni tohumlama uygulamaları dondurulmuş-çözülmüş semen payetleriyle intraservikal olarak gerçekleştirildi. Gebelik teşhisi için progesteron seviyelerini değerlendirmek amacıyla suni tohumlamadan 21 gün sonra kan örnekleri toplandı. CLIA yöntemi ile belirlenen gebelik oranı ve ortalama progesteron seviyesi ELFA yöntemi ile belirlenenlerden daha yüksek bulunmuştur. Ayrıca erken gebelik tespitinde gerçekten gebe hayvan sayısı duyarlılık, özgüllük ve doğruluk açısından önemli ölçüde daha yüksekti. CLIA yöntemi plazma progesteron seviyelerini ölçmek için hassas ve güvenilir bir yaklaşımdır. Çalışmada Kilis keçilerinde erken gebelik tespiti için değerlendirilen CLIA progesteron testi yönteminin üstün olduğu sonucuna varılmıştır.

Anahtar Kelimeler

• CLIA
• ELFA
• Gebelik
• Keçi
• Progesteron seviyeleri

Kaynakça

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