The Effect of Orally Administered Collagen on Testosterone Hormone and Sperm Count in Streptozotocin-Applied Rats

Yıl 2026, Cilt: 37 Sayı: 1, 18 - 21, 29.03.2026

Saadet Belhan , Ahmet Ufuk Kömüroğlu

https://doi.org/10.36483/vanvetj.1781364

https://izlik.org/JA89HH83GM

Öz

Diabetes mellitus is a common metabolic disorder characterized by chronic hyperglycemia resulting from impaired insulin secretion or action. Collagen, the most abundant structural protein in the body, plays a critical role in tissues such as skin, bone, cartilage, blood vessels, and testis. This study aimed to investigate the effects of oral collagen supplementation on serum testosterone levels and sperm count in diabetic rats. A total of 32 male Wistar albino rats were randomly assigned into four groups: Control, Diabetes mellitus, Diabetes mellitus+Collagen, and Collagen. Diabetes mellitus was induced via a single intraperitoneal injection of streptozotocin (55 mg/kg), while collagen peptide was administered daily by gastric gavage at a dose of 600 mg/kg for 28 days. Serum testosterone levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA). The results demonstrated a significant decrease in testosterone (p<0.05) and sperm count (p<0.001) in the Diabetes mellitus group, whereas the Diabetes mellitus+Collagen group exhibited partial improvement in both parameters. In the Collagen-only group, testosterone levels and sperm count were comparable to the Control group, indicating a limited effect of collagen under normal physiological conditions. These findings suggest that collagen may partially mitigate diabetes-induced testicular dysfunction by supporting the structural integrity of testicular tissue and promoting spermatogenesis. Overall, the results indicate that collagen supplementation could support testicular function under diabetic conditions, but further studies with larger sample sizes and longer durations are warranted to better elucidate its therapeutic potential.

Anahtar Kelimeler

Collagen , Diabetes mellitus , Rat , Sperm , Testosterone.

Streptozotosin Uygulanan Ratlarda Oral Yolla Verilen Kolajenin Testosteron Hormonu ve Sperm Sayısı Üzerindeki Etkisi

https://izlik.org/JA89HH83GM

Öz

Diyabetes mellitus, insülin salgısının veya etkisinin bozulmasından kaynaklanan kronik hiperglisemi ile karakterize yaygın bir metabolik bozukluktur. Vücutta en bol bulunan yapısal protein olan kolajen, deri, kemik, kıkırdak, kan damarları ve testis gibi dokularda kritik bir rol oynar. Bu çalışma, oral kolajen takviyesinin diyabetik sıçanlarda serum testosteron düzeyleri ve sperm sayısı üzerindeki etkilerini araştırmayı amaçlamıştır. Toplam 32 erkek Wistar albino sıçan, rastgele dört gruba ayrılmıştır: Kontrol, Diyabetes mellitus, Diyabetes mellitus+Kolajen ve Kolajen. Diyabetes mellitus, tek bir intraperitoneal streptozotosin enjeksiyonu (55 mg/kg) ile indüklenmiş, kolajen peptidi ise 28 gün boyunca günlük olarak gastrik gavaj yoluyla 600 mg/kg dozunda uygulanmıştır. Serum testosteron düzeyleri ELISA yöntemi ile ölçülmüştür. Sonuçlar, Diyabetes mellitus grubunda testosteron (p<0.05) ve sperm sayısında (p<0.001) anlamlı bir azalma olduğunu göstermiştir; buna karşın Diyabetes mellitus+Kolajen grubunda her iki parametrede kısmi bir iyileşme gözlenmiştir. Sadece Kolajen verilen grupta, testosteron düzeyleri ve sperm sayısı Kontrol grubu ile karşılaştırılabilir düzeyde bulunmuş ve normal fizyolojik koşullar altında kolajenin sınırlı bir etkisi olduğu görülmüştür. Bu bulgular, kolajenin testis dokusunun yapısal bütünlüğünü destekleyerek ve spermatogenezi teşvik ederek diyabete bağlı testiküler disfonksiyonu kısmen azaltabileceğini göstermektedir. Genel olarak, sonuçlar kolajen takviyesinin diyabetik koşullar altında testis fonksiyonunu destekleyebileceğini göstermektedir; ancak terapötik potansiyelini daha iyi anlamak için daha büyük örneklem büyüklükleri ve daha uzun süreli çalışmalar gerekmektedir.

Anahtar Kelimeler

Kolajen , Rat , Sperm , Şeker hastalığı , Testosteron.

Kaynakça

• Abdel-Wahab BA, El-Shoura EAM, Habeeb MS et al. (2025). Piperazine ferulate impact on diabetes-induced testicular dysfunction: Unveiling genetic insights, MAPK/ERK/JNK pathways, and TGF-β signaling. Naunyn Schmiedebergs Arch Pharmacol, 398(6), 6719–6737.
• AlTamimi JZ, AlFaris NA, Aljabryn DH et al. (2021). Ellagic acid improved diabetes mellitus-induced testicular damage and sperm abnormalities by activation of Nrf2. Saudi J Biol Sci, 28(8), 4300–4310.
• Andlib N, Sajad M, Kumar R, Thakur SC (2023). Abnormalities in sex hormones and sexual dysfunction in males with diabetes mellitus: A mechanistic insight. Acta Histochem, 125, 151974.
• Bondarenko LB, Shayakhmetova GM, Matvienko AV, Kovalenko VM (2012). Diabetes-mediated changes in rat type I collagen and spermatogenesis indices. Rom J Diabetes Nutr Metab Dis, 19(3), 245-254.
• Chen ZF, Shen YF, Gao DW et al. (2025). Metabolic pathways and male fertility: Exploring the role of Sertoli cells in energy homeostasis and spermatogenesis. Am J Physiol Endocrinol Metab, 329(2), E160–E178.
• Cheng CY, Wong EWP, Yan HHN, Mruk DD (2010). Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: New insights and advances. Mol Cell Endocrinol, 315(1–2), 49–56.
• Faheem H, Alawadhi R, Basha EH et al. (2025). Ameliorating immune-dependent inflammation and apoptosis by targeting TLR4/MYD88/NF-κB pathway by celastrol mitigates the diabetic reproductive dysfunction. Physiol Genomics, 57(3), 103–114.
• França LR, Avelar GF, Almeida FFL (2005). Spermatogenesis and sperm transit through the epididymis in mammals with emphasis on pigs. Theriogenology, 63(2), 300–318.
• Gregorič N, Šikonja J, Janež A et al. (2025). Semaglutide improved sperm morphology in obese men with type 2 diabetes mellitus and functional hypogonadism. Diabetes Obes Metab, 27, 519–528.
• Hadley MA, Dym M (1987). Immunocytochemistry of extracellular matrix in the lamina propria of the rat testis: Electron microscopic localization. Biol Reprod, 37(5), 1283–1289.
• Hasan BF, Hilal JA, Alwan NA (2023). Effect of oral administration of Collagen-α® on reproductive activity and growth efficiency of mature male rabbit. Iran J Ichthyol, Special Issue, 85–89.
• Huang R, Chen J, Guo B et al. (2024). Diabetes-induced male infertility: Potential mechanisms and treatment options. Mol Med, 30, 11.
• Imani M, Talebi AR, Fesahat F et al. (2021). Sperm parameters, DNA integrity, and protamine expression in patients with type II diabetes mellitus. J Obstet Gynaecol, 41(3), 439–446.
• Jena L, Kaur P, Singh T et al. (2024). Gene expression analysis in T2DM and its associated microvascular diabetic complications: Focus on risk factor and RAAS pathway. Mol Neurobiol, 61, 8656–8667.
• Jin T, Li F, Wei W et al. (2025). SDF2L1 downregulation mediates high glucose-caused Schwann cell dysfunction by inhibiting nuclear import of TFEB and CREB via KPNA3. Exp Neurol, 390, 115273.
• Kahsai TZ, Enders GC, Gunwar S et al. (1997). Seminiferous tubule basement membrane: Composition and organization of type IV collagen chains, and the linkage of alpha3(IV) and alpha5(IV) chains. J Biol Chem, 272(27), 17023–17032.
• Khalil ASM, Giribabu N, Yelumalai S et al. (2021). Myristic acid defends against testicular oxidative stress, inflammation, apoptosis: Restoration of spermatogenesis, steroidogenesis in diabetic rats. Life Sci, 278, 119605.
• Khordad E, Nikravesh MR, Jalali M, Fazel AR, Sankian M (2020). Diabetes up-regulated collagen IV and laminin α5 genes in mRNA and protein levels in seminiferous tubules of C57BL/6 adult mice. Cell Mol Biol (Noisy-le-Grand), 66(5), 162–168.
• Kiani M, Mehranjani MS, Shariatzadeh MA (2025). Myoinositol improves sperm parameters in diabetic rats by reducing oxidative stress and regulating apoptosis-related genes. J Mol Histol, 56, 165.
• Kumar V, Jain P, Rathore K, Ahmed Z (2016). Biological evaluation of Pupalia lappacea for antidiabetic, antiadipogenic, and hypolipidemic activity both in vitro and in vivo. Scientifica, 2016, 1–10.
• Lotti F, Maggi M (2023). Effects of diabetes mellitus on sperm quality and fertility outcomes: Clinical evidence. Andrology, 11(3), 399–416.
• Lovic D, Piperidou A, Zografou I et al. (2020). The growing epidemic of diabetes mellitus. Curr Vasc Pharmacol, 18(2), 104–109.
• Maranduca I, Serban G (2022). Effective dose of streptozotocin to induce diabetes mellitus in albino Wistar rats. Farmacia, 70(4), 448–455.
• Shoulders MD, Raines RT (2009). Collagen structure and stability. Annu Rev Biochem, 78, 929–958.
• Siu MKY, Cheng CY (2008). Extracellular matrix and its role in spermatogenesis. In: Cheng CY (ed) Molecular mechanisms in spermatogenesis (pp 74–91). Springer.
• Siu MK, Lee WM, Cheng CY (2003). The interplay of collagen IV, tumor necrosis factor-alpha, gelatinase B (matrix metalloprotease-9), and tissue inhibitor of metalloproteases-1 in the basal lamina regulates Sertoli cell-tight junction dynamics in the rat testis. Endocrinology, 144(1), 371–387.
• Skinner MK (2005). Regulation of primordial follicle assembly and development. Hum Reprod Update, 11(5), 461–471.
• Soetan OA, Ajao FO, Ajayi AF (2024). Erythritol attenuates testicular dysfunction in diabetic rat via suppression of oxidative stress, inflammation and apoptosis. Biochem Biophys Res Commun, 690, 149254.
• Sonmez M, Turk G, Yüce A (2005). The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats. Theriogenoly, 63(6), 2063–2072.
• Tsilibary EC (2003). Microvascular basement membranes in diabetes mellitus. J Pathol, 200(4), 537–546.
• Xu R, Wang F, Zhang Z et al. (2023). Diabetes-induced autophagy dysregulation engenders testicular impairment via oxidative stress. Oxid Med Cell Longev, 2023, 4365895.
• Xu Y, Hu P, Chen W et al. (2025). Testicular fibrosis pathology, diagnosis, pathogenesis, and treatment: A perspective on related diseases. Andrology, 13(6), 1322–1332.
• Vijayan DK, Raman SP, Dara PK et al. (2022). In vivo anti-lipidemic and antioxidant potential of collagen peptides obtained from great hammerhead shark skin waste. J Food Sci Technol, 59(3), 1140–115.