Detection of Microsatellite Polymorphisms in Van Cats on Some Phenotype Characteristics

Öz

Using molecular genetic markers, it is possible to determine the desired genotypes while preserving the genetics of endangered species, thereby increasing or decreasing genetic diversity in populations. The Van cat is one of the endangered species living in the Lake Van region. Microsatellite markers are commonly used to estimate genetic diversity and genetic differentiation in a population. The aim of this study was to characterize the genetic structure and determine the genetic differentiation of the protected Van cat population based on the phenotypes of eye color, coat length and three points on the head. For this purpose, 25 male and 41 female cats were selected and four microsatellite loci (FCA 176, 478, 547, 688) were selected from the whole genome of the domestic cat. Based on the results of the statistical analysis, it was found that the influence of the 4 microsatellites on gender was not significant (p0.05). In addition, loci FCA176, 478, 547 and 688 had no effect on eye color and distribution. Although coat length and locus FCA176 were not statistically significant, it had a value of p=0.0534, which is very close to the p-value of 0.05. An important result was that the association between the three-point birthmark on the head and the FCA478 marker was highly significant (p≤0.05).

Anahtar Kelimeler

• Genetic polymorphisms
• Genetic resources
• Microsatellite markers
• Van cats

Destekleyen Kurum

This study was financially supported by the Van Yüzüncü Yıl University Scientific Research Projects Directorate under project number 2008-ZF-069

Proje Numarası

2008-ZF-069

Etik Beyan

This study was conducted with the approval document of the Local Ethics Committee for Animal Experiments of Yüzüncü Yıl University (YÜHADYEK) dated 09.02.2009 with the number B.30.2.YYÜ.0.0506.00/300-0053. Thus, no invasive procedures were performed on the arms and no ani¬mal experiments were performed according to the legal definitions in Europe (Subject 5f of Article 1, Chapter I of Directive 2010/63/eu of the European Parliament and of the Council). Furthermore, we declare that animal welfare and safety were priorities in this study and that the Helsinki principle and protocol were carefully followed.

Van Kedilerinde Mikrosatellit Polimorizmlerinin Bazı Fenotip Karakteristiklerinde Saptanması

Öz

Moleküler genetik belirteçler kullanılarak, nesli tükenme tehlikesi altında olan türlerin genetik yapısı korunurken istenilen genotiplerin belirlenmesi ve böylece popülasyonlarda genetik çeşitliliğin artırılması veya azaltılması mümkün olmaktadır. Van kedisi, Van Gölü havzasında yaşayan ve koruma altındaki türlerden biridir. Mikrosatellit belirteçler, bir popülasyondaki genetik çeşitliliği ve genetik farklılaşmayı tahmin etmek amacıyla yaygın olarak kullanılmaktadır. Bu çalışmanın amacı, Van kedisi koruma popülasyonunun genetik yapısını karakterize etmek ve göz rengi, tüy uzunluğu ve baş üzerinde bulunan üçlü benek fenotiplerine dayalı olarak genetik farklılaşmayı belirlemektir. Bu amaçla, 25 erkek ve 41 dişi kedi seçilmiş olup evcil kedinin tüm genomunda yer alan dört mikrosatellit lokusu (FCA176, 478, 547, 688) incelenmiştir. İstatistiksel analiz sonuçlarına göre, dört mikrosatellitin cinsiyet üzerine etkisi önemsiz bulunmuştur (p≥0.05). Ayrıca FCA176, 478, 547 ve 688 lokuslarının göz rengi ve dağılımı üzerinde herhangi bir etkisinin olmadığı belirlenmiştir. Tüy uzunluğu ile FCA176 lokusu arasındaki ilişki istatistiksel olarak anlamlı bulunmamış olmakla birlikte p=0.0534 değeri, 0.05 anlamlılık düzeyine oldukça yakın çıkmıştır. Çalışmanın önemli bir sonucu ise baş üzerinde bulunan üçlü benek ile FCA478 belirteci arasındaki ilişkinin yüksek derecede anlamlı olarak bulunmuştur (p≤0.05).

Anahtar Kelimeler

• Genetik kaynaklar
• Genetik polimorfizm
• Mikrosatellit belirteçler
• Van kedileri

Destekleyen Kurum

This study was financially supported by the Van Yüzüncü Yıl University Scientific Research Projects Directorate under project number 2008-ZF-069

Proje Numarası

2008-ZF-069

Etik Beyan

This study was conducted with the approval document of the Local Ethics Committee for Animal Experiments of Yüzüncü Yıl University (YÜHADYEK) dated 09.02.2009 with the number B.30.2.YYÜ.0.0506.00/300-0053. Thus, no invasive procedures were performed on the arms and no ani¬mal experiments were performed according to the legal definitions in Europe (Subject 5f of Article 1, Chapter I of Directive 2010/63/eu of the European Parliament and of the Council). Furthermore, we declare that animal welfare and safety were priorities in this study and that the Helsinki principle and protocol were carefully followed.

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