Origanum vulgare Essential Oil Demonstrates In Vivo and In Silico Activity Against a Mouse Nematode Syphacia obvelata (Rudolphi, 1802)

Öz

Oxyurid nematode infections are common in animals and often recurrent, necessitating the development of new anthelmintic agents. Origanum vulgare L. subsp. hirtum essential oil (OEO) was evaluated for its nematocidal effects against the Syphacia obvelata nematode in naturally infected mice. Mice received oral OEO at 300 and 600 mg/kg doses, with egg counts monitored via the perianal tape method. Gas chromatography identified carvacrol (84.0%) as the dominant compound, while in silico docking revealed β-caryophyllene exhibited strong binding affinities to β-tubulin and carnitine palmitoyltransferase 2, key nematode drug targets. OEO treatment significantly reduced parasite egg counts by up to 98% at 7 days post-treatment, with sustained effects at 14 days. These findings suggest that OEO, particularly β-caryophyllene, possesses promising anthelmintic activity against oxyurid nematodes, as a preliminary study. Further studies are warranted to explore its therapeutic potential and mechanisms of action.

Anahtar Kelimeler

• Origanum vulgare
• Anthelmintic properties
• Essential oils
• Molecular docking
• Nematocidal activity

Etik Beyan

This study was conducted with the permission of Bursa Uludağ University, Animal Experiments Local Ethics Committee on 11.07.2017 with the number 2017-10/07.

Teşekkür

We are grateful to our esteemed and deceased Professor, Dr Hulusi Malyer, who helped to obtain Origanum essential oil from Eskişehir Anadolu University. We also thank Assoc. Prof. Dr. Nurhayat Dalkıran for assistance with statistical analysis, and Dr. Nurcihan Güven for for assistance in the laboratory.

Origanum vulgare Esansiyel Yağının Fare Nematodu Syphacia obvelata'ya (Rudolphi, 1802) Karşı İn-Vivo ve İn-Siliko Etkisi

Öz

Oksyurid nematod enfeksiyonları hayvanlarda yaygındır ve sıklıkla tekrarlar. Bu nedenle yeni anthelmintik ajanların geliştirilmesi gerekmektedir. Origanum vulgare L. subsp. hirtum uçucu yağı (OEO), Syphacia obvelata nematodu ile doğal enfekte farelerde, nematosidal etkiler açısından değerlendirilmiştir. Farelere 300 ve 600 mg/kg dozlarında oral OEO verilmiş ve yumurta sayıları perianal bant yöntemi ile izlenmiştir. Gaz kromatografisi, karvakrolü (%84.0) baskın bileşik olarak tanımlarken, in-siliko yerleştirme, β-karyofilenin β-tubulin ve karnitin palmitoil transferaz 2'ye, yani nematod ilaçlarının temel hedeflerine güçlü bağlanma eğilimi gösterdiğini ortaya koymuştur. OEO tedavisi, tedaviden sonraki 7. günde parazit yumurta sayısını %98'e kadar önemli ölçüde azaltmış ve etkilerin 14. günde devam etmesini sağlamıştır. Bu bulgular, OEO'nun, özellikle β-karyofilenin, oksyurid nematodlara karşı umut verici bir anthelmintik aktiviteye sahip olduğunu bir ön çalışma olarak göstermektedir. Terapötik potansiyelini ve etki mekanizmalarını araştırmak için daha fazla çalışmaya ihtiyaç vardır.

Anahtar Kelimeler

• Origanum vulgare
• Esansiyel yağ
• Moleküler yerleştirme
• Nematosidal aktivite
• Anthelmintik özellik

Etik Beyan

Bu çalışma, Bursa Uludağ Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’ndan 11.07.2017 tarihinde 2017-10/07 sayılı izin alınarak yapılmıştır.

Teşekkür

Origanum vulgare uçucu yağının Eskişehir Anadolu Üniversitesinden elde edilmesini sağlayan merhum Prof.Dr. Hulusi Malyer’e, yağın içerik tayininin yapılmasında laboratuvardaki yardımlarından dolayı Dr. Nurcihan Güven’e ve istatistik analizlerin yapılmasında Prof.Dr. Nurhayat Dalkıran’a teşekkür ederiz.

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