Bütilparaben Uygulamasını Sıçan Beyin ve Serumunda Oksidatif Stres Belirteçleri Üzerine Etkileri

Öz

Parabenler, özellikle ilaç, kozmetik ve gıda ürünleri olmak üzere çeşitli alanlarda koruyucu olarak kullanılmaktadır. Son zamanlarda yapılan çalışmalar, parabenlerin biyolojik sistemlerdeki oksidatif stres mekanizmalarını etkileyebileceğini ve hücresel hasara neden olabileceğini göstermektedir. Çalışmamızda, sıçanlarda beyin dokusu, serum ve eritrositlerdeki oksidatif stres parametreleri üzerinde bütilparaben (BP) uygulamasının etkilerini değerlendirerek BP’nin olası biyolojik etkilerini daha iyi anlamayı amaçladık. Bu amaçla, 4 farklı gruba ayrılan 28 Wistar Albino sıçana oral gavaj yoluyla 0, 100, 200, 400 mg/kg/gün dozlarında BP verilmiştir. Sıçanların beyin, serum ve eritrositlerinde katalaz (CAT), glutatyon (GSH), malondialdehit (MDA), total antioksidan kapasite (TAK), total oksidan kapasite (TOK) ve oksidatif stres indeksi (OSİ) gibi oksidatif stres parametreleri ölçülmüştür. Elde edilen sonuçlara göre, BP ile tedavi edilen gruplarda kontrol grubuna kıyasla beyin dokusunda MDA düzeylerinin önemli ölçüde arttığı gözlemlenmiştir (p<0.05). Ancak, eritrositlerdeki GSH düzeyleri ve CAT aktivitesi ile serumdaki MDA, TAS, TOS ve OSI düzeyleri açısından gruplar arasında önemli bir fark gözlemlenmemiştir (p>0.05). Bu bulgular, BP'nin lipid peroksidasyonunu artırarak oksidatif stresi etkilediğini, ancak genel oksidatif dengede büyük değişiklikler yaratmadığını, özellikle de BP'nin beyin bölgelerinde tespit edildiği ve beynin lipid peroksidasyonuna duyarlı olduğu göz önüne alındığında, kanıtlamıştır. Bu çalışma, BP'nin oksidatif stres üzerindeki etkilerini daha ayrıntılı olarak anlamak için yapılacak daha ileri araştırmalara katkıda bulunmayı amaçlamaktadır.

Anahtar Kelimeler

• Beyin
• Bütilparaben
• MDA
• Oksidatif stres

The Effects of Butylparaben Administration on Oxidative Stress Markers in Rat Brain and Serum

Öz

Parabens are used for preservative purposes in various fields, especially in pharmaceutical, cosmetic and food products. Recent studies suggest that parabens may affect oxidative stress mechanisms in biological systems and may cause cellular damage. This experiment was created to better understand the possible biological effects of butylparaben (BP) by evaluating the effects of BP administration on oxidative stress parameters in brain tissue, serum and erythrocytes in rats. For this purpose, 28 Wistar Albino rats divided into 4 different groups were administered BP at doses of 0, 100, 200, 400 mg/kg/day by oral gavage. Oxidative stress parameters such as total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), glutathione (GSH) and malondialdehyde (MDA) and catalase (CAT) were measured in the brain, serum and erythrocytes of rats. The findings revealed a statistically notable increase in MDA concentrations within the brain tissue of the BP-treated groups evaluated to the control group (p<0.05). Nonetheless, the groups did not show any statistically considerable variations in erythrocyte GSH levels, CAT activity, or in serum MDA, TOS, TAS, and OSI parameters (p>0.05). These findings provided proof that BP affects oxidative stress by increasing lipid peroxidation but does not produce large changes in the overall oxidative balance, especially since BP was detected in brain regions and the brain is sensitive to lipid peroxidation. This study aims to contribute to further research to understand the effects of BP on oxidative stress in more detail.

Anahtar Kelimeler

• Brain
• Butylparaben
• MDA
• Oxidative stress

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