Investigation of the Effects of Carvacrol on Oxytetracycline-Induced Kidney Damage

Öz

The main objective of this study was to assess the antioxidant capacity and the protective impact of carvacrol (CRV) against oxytetracycline (OXI)-mediated nephrotoxicity in albino rats. CRV, a naturally occurring phenolic compound found in various plant species, has significant antioxidant capacity, the ability to mitigate inflammatory processes, and tumor proliferation inhibiting properties. The study evaluated the efficacy of CRV in reducing oxidative stress-induced kidney damage in albino rats. Findings showed that CRV administration significantly reduced the kidney damage relative to the control group. Evaluation of the enzymatic antioxidant profile indicated a significant decrease in the activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) enzymes and GSH levels in the OXI-treated group compared to the CRV group. Furthermore, CRV treatment decreased malondialdehyde (MDA), a key biomarker of lipid peroxidation. The protective properties of CRV were confirmed by histological examination of the kidney. CRV treatment markedly suppressed pro-inflammatory molecules, specifically diminishing the expression of interleukin-1 beta (IL-1β) and mitigating nuclear factor kappa-B (NF-κB) activation. Furthermore, CRV administration attenuated OXI-induced increases in the proapoptotic proteins Bax and Caspase-3, while increasing the transcriptional activity of the antiapoptotic gene B-cell lymphoma-2 (Bcl-2). CRV diminished OXI-induced kidney tissue damage by decreasing Kidney Injury Molecule-1 (KIM-1) and increasing Aquaporin-2 (AQP-2) levels. The study results indicate that CRV administration can alleviate tissue damage in the kidney caused by OXI toxicity.

Anahtar Kelimeler

• Albino rat
• antioxidant activity
• carvacrol
• kidney function
• lipid peroxidation
• oxytetracycline

Oksitetrasiklin Kaynaklı Böbrek Hasarı Üzerine Karvakrolün Etkilerinin Araştırılması

Öz

Bu çalışmanın amacı, albino sıçanlarda oksitetrasiklin (OXI) kaynaklı nefrotoksisiteye karşı karvakrolün (CRV) antioksidan potansiyelini ve koruyucu rolünü araştırmaktır. Çeşitli bitki türlerinde doğal olarak bulunan fenolik bir bileşik olan CRV’nin önemli antioksidan, anti-inflamatuar ve antikanser özellikleri vardır. Çalışmada, albino sıçanlarda oksidatif stres kaynaklı böbrek hasarını azaltmada CRV'nin etkinliği değerlendirilmiştir. Bulgular, CRV uygulamasının böbrek hasarını kontrol grubuna kıyasla önemli ölçüde azalttığını göstermiştir. OXI uygulanan grupta, CRV uygulanan gruba kıyasla süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ile katalaz (CAT) da dâhil antioksidan etkinliği olan enzimlerin aktivitelerinde ve GSH seviyelerinde önemli bir azalma gözlenmiştir. Ayrıca, CRV tedavisi, lipid peroksidasyonunun önemli bir biyobelirteci olan malondialdehit (MDA) düzeyini düşürmüştür. CRV'nin koruyucu özellikleri, böbreğin histolojik incelemesiyle doğrulanmıştır. CRV tedavisi, nükleer faktör kappa-B (NF-κB) ile interlökin-1 beta (IL-1β) gibi inflamasyon medyatörleri önemli ölçüde azalttı. Ayrıca, CRV uygulaması, proapoptotik proteinler Bax ve Kaspaz-3'te OXI kaynaklı artışları azaltırken, antiapoptotik belirteç B hücreli lenfoma-2 (Bcl-2) ekspresyonunu artırdı. CRV, Böbrek Hasarı Molekülü-1'i (KIM-1) azaltarak ve Aquaporin-2 (AQP-2) seviyelerini artırarak OXI kaynaklı böbrek doku hasarını azalttı. Çalışma sonuçları, CRV uygulamasının OXI toksisitesinden kaynaklanan böbrek doku hasarını hafifletebileceğini göstermektedir.

Anahtar Kelimeler

• Albino sıçan
• antioksidan aktivite
• böbrek fonksiyonu
• karvakrol
• lipit peroksidasyonu
• oksitetrasiklin.

Kaynakça

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