The Effect of a Highly Saturated Fat Diet and Intermittent Fasting Diet on Experimental Colon Cancer Development and Some Serum Inflammation Markers in Rats, 1 Adiponectin and Lipid Metabolism

Öz

In this study, the effects of two-days food restriction per week (intermittent feeding) on the serum adiponectin and lipid profiles in rats subjected to high fat diet and exposed to Dimetilhidrazin (DMH), a potent colon carcinogen, were investigated. The Wistar albino rats were divided into two groups as experimental and control. After two weeks period of pre-feeding with high fat diet for adaptation and adjustment, the both groups were fed with the same diet for a course of 10 weeks. Experimental group was deprived of food twice a week only. Both groups received two doses of Dimethylhydrazine (DMH), 25 and 125 mg/kg, a chemical colonotropic carcinogen, at the beginning of the experiment. At the end of the study, adiponectin, triglyceride, cholesterol, VLDL and HDL were measured. In the experimental group, adiponectin, cholesterol and VLDL levels were found to be higher than control group, though not statistically significant, but triglyceride levels were significantly lower than the control group (p<0.05). However, HDL levels were lower than the control group insignificantly. As a result, this study suggest that it could be possible to modulate obesity, which is a chronic inflammation state and trigger of many metabolic abnormalities, and to prevent some metabolic pathologies by just 2 days intermittent fasting regime without stopping high fat diet feeding

Anahtar Kelimeler

• Highly fat diet, Adiponectin, Trigliserid, Obesity, Rat

Sıçanlarda Yüksek Oranda Doymuş Yağlı Diyet ile Aralıklı Beslemenin Deneysel Kolon Gelişimi ve Bazı Serum İnflamasyon Markırları Üzerine Etkisi 1 Adiponektin ve Lipid Metabolizması

Öz

Bu çalışmada yüksek oranda yağlı diyetle beslenen ve kolonotropik kanserojen maddeye maruz bırakılan ratlarda, haftada sadece 2 gün tam gıda kısıtlamasının (modifiye aralıklı besleme) serum adiponektin ve lipit metabolizması üzerine etkileri incelendi. Wistar albino ratlar her iki grupta 15 rat olacak şekilde deney (aralıklı besleme) ve kontrol olmak üzere iki gruba ayrıldı. Her iki grupta da yüksek yağlı diyetle 2 hafta ön besleme uygulaması sonrasında 10 deneme haftası boyunca yüksek yağlı beslemeye devam edildi. Deney grubu haftada sadece 2 gün tamamen yemden uzak tutuldu. Her iki gruba da deneme başında kolonotropik kimyasal karsinojen olan Dimetilhidrazin (DMH) 2 doz halinde 25 ve 125 mg/kg subkutan enjeksiyon şeklinde yapıldı. Çalışma sonunda serumda adiponektin, trigliserit, kolesterol, Vldl ve Hdl parametrelerine bakıldı. Deney grubunda adiponektin, kolesterol ve Vldl değerleri kontrol grubuna göre yüksek, trigliserit (p

Anahtar Kelimeler

• Yüksek Yağlı Diyet, Adiponektin, Trigliserit Obezite, Rat

Kaynakça

1. Belkacemı L, Selselet-Attou G, Bulur N, Louchamı K, Sener A, Malaısse WJ (2011). Intermittent fasting modulation of the diabetic syndromein sand rats. III. Post-mortem investigations. Int J Mol Med, 27, 95-102,
2. Bordone L, Guarente L (2005). Calorie restriction, SIRT1 and metabolism: understanding longevity. Nat Rev Mol Cell Biol, 6, 298–305.
3. Day SD, Enos RT, McClellan JL, Steiner JL, Velazquez KT, Murphy EA (2013). Linking inflammation to tumorigenesis in a mouse model of high-fat-diet-enhanced colon cancer. Cytokine, 64 (1), 454-462.
4. Faloia EG, Michetti M, de Robertis M P, Luconi G, Furlani MB (2012). Inflammation as a link between obesity and metabolic syndrome. J Nutr Met, Vol 2012, ID 476380, 7 pages.
5. Fontana L, Klein S (2007). Aging, adiposity, and calorie restriction. JAMA , 297, 986–994.
6. Gregor MF, Hotamisligil GS (2011). Inflammatory mechanisms in obesity. Annu Rev Immunol, 29, 415-45.
7. Hotamisligil GS, P Arner JF, Caro RL, Atkinson BM (1995). Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance. J Clin Invest, 95 (5), 2409–2415.
8. Hsieh EA, Chai CM, Hellerstein MK (2005). Effects of caloric restriction on cell proliferation in several tissues in mice: role of intermittent feeding. Am J Physiol Endocrinol Metab, 288, E965–E972.

9. Jemal A, Siegel R, Xu J, Ward E (2010). Cancer statistics. CA Cancer J Clin, 60, 277-300.
10. Li L, Wang Z, Zuo Z (2013). Chronic ıntermittent fasting ımproves cognitive functions and brain structures in mice. PLoS ONE 8(6): e66069.
11. Li S, Shin HJ, Ding EL, van Dam RM (2009). Adiponectin levels and risk of type 2 diabetes: a systematic review and meta-analysis. JAMA, 302, 179-188.
12. Luft VC, Schmidt MI, Pankow JS, et al (2013). Chronic inflammation role in the obesity-diabetes association: a case-cohort study. Diabetol Metab Syndr, 27, 5, 1,31.
13. Maeda N, Shimomura I, Kishida K, et al (2002). Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med, 8731-737.
14. Matsubara M, Maruoka S, Katayose S ( 2002). Inverse relationship between plasma adiponectin and leptin concentration normal-weight and obese women. Eur J Endocrinol, 147, 173-180.
15. Meier U, Gressner AM (2004). Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem, 50, 1511-1525.
16. Park S, Park NY, Valacchi G, Lim Y (2012). Calorie restriction with a highfat diet effectively attenuated ınflammatory response and oxidative stress-related markers in obese tissues of the high diet fed rats. Mediat Inflamm, Vol 2012, Article ID: 984643.
17. Radhakishun N, Blokhuis C, Vliet MV, et al (2014). Intermittent fasting during Ramadan causes a transient increase in total, LDL, and HDL cholesterols and hs-CRP in ethnic obese adolescents. Eur J Pediatr, 173, 1103–1106.
18. Rao CV, Hirose Y, Indranie C, Reddy BS (2001). Modulation of experimental colon tumorigenesis by types and amounts of dietary fatty acids. Cancer Res. 61 (5), 1927-1933.
19. Sadiya A, Ahmed S, Siddieg HH, Babas IJ, Carlsson M (2011). Effect of Ramadan fasting on metabolic markers, body composition, and dietary intake in Emiratis of Ajman (UAE) with metabolic syndrome. Diabetes Metab Syndr Obes, 4, 409–416.
20. Salehi M, Neghab M (2007). Effects of fasting and a medium calorie balanced diet during the holy month Ramadan on weight, BMI and some blood parameters of overweight males. Pak J Biol Sci, 10, 6, 968– 971.
21. Stienstra R, Duval C, Müller M, Kersten S (2007). Obesity, and Inflammation. PPAR Res. Article ID: 95974.
22. Syrenicz A, Garanty-Bogacka B, Syrenicz M, Gebala A, Walczak M (2006). Low grade systemic inflammation and the risk of type 2 diabetes in obese children and adolescents. Neuro Endocrinol Lett, 27 (4), 453–458.
23. Varady KA, Bhutani S, Church EC, Klempel MC (2009). Short-term modified alternate-day fasting: a novel dietary strategy for weight loss and cardioprotection in obese adults. Am J ClinNutr, 90, 1138–1143
24. Wan R, Ahmet I, Brown M, et al (2010). Cardioprotective effect of intermittent fasting is associated with an elevation of adiponectin levels in rats. J Nutr Biochem. 21 (5), 413-417.
25. Yang Y, Qin J, Ke B, Zhang J, Shi L, Li Q ( 2013). Effect of Linguizhugan decoction on hyperlipidemia rats with intermittent fasting. J Tradit Chin Med, 33 (2), 250-252.