The Effect of Octyl-Cyanoacrylate in Wound Healing at Closing the Experimentally Formed Skin Incision in Rabbits

Öz

In this study, it was aimed to evaluate the effect of tissue adhesive octyl-cyanoacrylate (OCA) at wound healing in closing the incisional skin wounds in rabbits. 24 healthy rabbits were used as study material. Two parallel full-thickness skin incisions with 6 cm length were performed under aseptic conditions on the left and right sides of each experimental rabbit dorsum and immediately subcutaneous connective tissue of all wounds were sutured by simple continuous suture pattern with 3/0 chromic gut. The right side skin incision (control group) sutured by seven simple sutures with 3/0 silk. The skin incisions at the left (OCA group) were closed with OCA tissue adhesive after approximate the wound edges with forceps and fingers. Six rabbits were anesthetized on days 3, 7, 14 and 21 after surgery. Skin wounds were removed both from the OCA and suture applied wounds which was 3 cm width and 6 cm length. They were used for histopathologic evaluation, tensile strength and hydroxyproline measurement. It was macroscopically observed that the inflammatory reaction and scab formation in the OCA group was less and the scar formation was similar and better in some regions compared to the control group.  It was also microscopically determined in the OCA group that the inflammatory reaction on postoperative days of 3 and 7 was less formed and the collagen formation was less on postoperative days of 14 and 21 compared to the control group. The wound tensile strength didn’t statistically different between the groups. It was determined that the hydroxyproline levels in OCA group were higher on postoperative days of 3 and 7 and there was no difference on postoperative days of 14 and 21. In conclusion, OCA tissue adhesive could be used in closing the incisional skin wounds in the small animal together with the subcutaneous suture.

Anahtar Kelimeler

• Octyl-cyanoacrylate,Wound healing,Rabbit

Tavşanlarda Deneysel Olarak Oluşturulan Deri Ensizyonlarının Kapatılmasında Oktil-Siyanoakrilatın Yara İyileşmesi Üzerine Etkisi

Öz

Bu çalışmada tavşanlarda oluşturulan ensizyonel deri yaralarının kapatılmasında oktil-siyanoakrilat (OCA) doku yapıştırıcısının yara iyileşmesine etkilerinin araştırılması amaçlandı. Çalışmanın materyalini 24 adet sağlıklı tavşan oluşturdu. Tavşanların bel omurları çizgisinin iki yanında ve birbirine paralel 6 cm uzunluğunda tam kalınlıkta deri ensizyonları oluşturuldu. Tüm yaraların deri altı bağ dokusuna 3/0 krome katgüt iplik ile basit sürekli dikiş uygulandı. Sağ taraftaki deri ensizyonları (kontrol grubu), 3/0 ipek iplikle basit ayrı dikiş uygulanarak kapatıldı. Sol taraftaki deri ensizyonları ise (çalışma grubu), yara dudakları forseps veya parmaklarla karşı karşıya getirildikten sonra OCA doku yapıştırıcısı ile kapatıldı. Postoperatif 3, 7, 14 ve 21. günlerde 6’şar adet tavşan anestezi edilerek, histopatolojik değerlendirme, gerilim direnci ve hidroksiprolin ölçümünde kullanılmak üzere hem OCA hem de dikiş uygulanan yara hattını içine alacak şekilde 3 cm genişliğinde ve 6 cm uzunluğunda dokular alındı. Makroskobik olarak çalışma grubunda kontrol grubuna göre yangısal reaksiyonun ve kabuk oluşumunun daha az, skar oluşumunun ise bazı bölgelerde benzer ve bazı bölgelerde ise daha iyi olduğu gözlendi. Mikroskobik olarak çalışma grubunda kontrol grubuna göre postoperatif 3. ve 7. günlerde yangısal reaksiyonun daha az şekillendiği ve postoperatif 14. ve 21. günlerde kollajen yapının daha az olduğu belirlendi. Yara gerilim direncinin gruplar arası istatistiksel değerlendirmede farklılık arz etmediği belirlendi. Hidroksiprolin düzeylerinin çalışma grubunda kontrol grubuna göre postoperatif 3. ve 7. günlerde yüksek olduğu, 14. ve 21. günlerde ise farklılık arz etmediği saptandı. Sonuç olarak; küçük hayvanlarda ensizyonel deri yaralarının kapatılmasında OCA doku yapıştırıcısının deri altı dikişi ile birlikte hareketsiz bölgelerde kullanılabileceği kanısına varıldı. 

Anahtar Kelimeler

• Oktil-siyanoakrilat,Yara iyileşmesi,Tavşan

Kaynakça

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