Identification of Glycoproteins in Mucous Cells of Epidermis and Gill of Tinca tinca Linnaeus, 1758 (Cypriniformes: Cyprinidae)

Öz

The present article reports of the glycoproteins in the mucous cells of gill of the Tinca tinca. As material, twenty five uninfected Tinca tinca were used. The gills were rapidly excised and fixed by immersion in 10% buffered formalin for light microscopic studies. The samples were routinely processed and embedded in parafin. Histochemical techniques were performed for the density and differentiation of carbohydrate moieties. Histochemical analysis showed that the gills mucous content included acidic (AB pH 2.5 +), neutral (PAS +), neutral or acid-rich (PAS/AB pH 2.5 +), strong acid sulphated (AF +) and strong sulphated (AF/AB pH 2.5 +) glycoproteins (GPs).

Anahtar Kelimeler

• Histochemistry,Gill,Tinca tinca

Kadife Balığının (Tinca tinca) Linnaeus, 1758 (Cypriniformes: Cyprinidae) Solungaç Epitel Mukus Hücrelerinde Glikoproteinlerin Belirlenmesi

Öz

Bu çalışmada kadife balığı (Tinca tinca) solungaç epitelindeki mukus hücrelerinde glikoproteinlerin belirlenmesi rapor edilmiştir. Materyal olarak 25 adet kadife balığı kullanıldı. Işık mikroskobik incelemeler için solungaçlar %10’luk formaldehit içerinde tespit edildi. Örnekler rutin doku takibinden geçirilip parafinde bloklandı. Parafin bloklardan karbonhidrat bileşenlerinin yoğunluğunu ve farklılığını belirlemek için histokimyasal teknikler uygulandı. Solungaç mukus içeriğinin asidik (AB pH 2.5 +), nötral (PAS +), nötral-asidik (PAS/AB pH 2.5 +), güçlü asidik sülfatlı (AF +) ve güçlü sülfatlı (AF/AB pH 2.5 +) glikoprotein olduğu gözlendi.

Anahtar Kelimeler

• Histokimya,Solungaç,Tinca tinca

Kaynakça

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