Confocal Images of The Colocalization and Upregulation of Vasotocin and Galanin After Water Deprivation in Roosters

Öz

The aim of this study was to investigate the effects of dehydration on galanin and vasotocin colocalization and galanin expression in the hypothalamo-neurohypophysial system. Adult (age: 22 to 35 weeks) roosters were used and deprived of drinking water for 48 hours in this study. The effects of dehydration were examined by measuring plasma osmolality, plasma levels of potassium, sodium and plasma vasotocin concentration. Double staining immunohistochemistry combined with confocal laser microscopy was utilized to determine the effects of dehydration on hypothalamic magnocellular neurons in the supraoptic and paraventricular nucleus and to verify the extend of colocalization. The results indicate that 48 hours water deprivation caused a moderate dehydration and significantly elevation of plasma osmolality, plasma sodium and vasotocin concentration. Furthermore, the colocalization of galanin and vasotocin was imaged in response to water deprivation only in supraoptic nucleus of hypothalamus. In this study, a close functional link between galanin and vasotocin neurons was found in all dehydrated animals. The simultaneous upregulation and the colocalization of these peptides in response to water deprivation may indicate a site specific control of vasotocin by galanin.

Anahtar Kelimeler

• Colocalization,Galanin,Vasotocin,Water Deprivation

Kaynakça

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