Evaluation of intercellular adhesion molecule-1 (ICAM-1), Tumor necrosis factor α (TNF-α), Interleukins (IL-6, IL-8) and C-reactive protein (CRP) levels in neonatal calves with presumed septicemia

Öz

In this study; intercellular molecule-1 (ICAM-1), Tumor necrosis factor α (TNF-α), Interleukin-6 (IL-6), Interleukin-8 (IL-8) and C-reactive protein (CRP) levels were evaluated in neonatal calves with presumed septicemia. The material of this study consisted of a total of 30 calves. Study group consisted of 20 septicemic calves and 10 clinically healthy calves and with same breed and weight and their ages were between 1 and 10 days. The statistical analysis indicated that leukocyte (WBC), red blood cell (RBC), hematocrit (Hct) hemoglobin (Hb) and mean corpuscular haemoglobin concentration (MCHC) values in neonatal calves with presumed septicemia were higher than those of the control group (P<0.05), whereas PLT values of neonatal calves with septicemia were lower than those of the control group (P<0.05). The levels of ICAM-1, TNF-α, IL-6, IL-8 and CRP values in septicemic calves were higher than those of the control group (P<0.05). As a result, it was concluded that analyzes of TNF-α, IL-6, IL-8 and particularly ICAM-1 concentrations may be considered in evaluating the severity and prognosis of patients with septicemia, as well as monitoring the efficacy of the treatment of the disease.

Anahtar Kelimeler

• Calf
• Septicemia
• Acute Phase Proteins
• Cytokines
• ICAM

Septisemi Şüpheli Yenidoğan Buzağılarda Hücrelerarası Adhezyon Molekül-1 (ICAM-1), Tümör nekroz faktörü α (TNF-α), İnterlökinler (IL-6, IL-8) ve C-reaktif protein (CRP) Seviyelerinin Değerlendirilmesi

Öz

Bu çalışmada; septisemi şüpheli yenidoğan buzağılarda hücreler arası molekül-1 (ICAM-1), Tümör nekroz faktörü α (TNF-α), İnterlökin-6 (IL-6), İnterlökin-8 (IL-8) ve C-reaktif protein (CRP) düzeyleri değerlendirildi. Bu çalışmanın materyalini aynı cins ve kiloya sahip ve yaşları 1-10 gün arasında değişen 20 septisemik buzağı ile 10 sağlıklı buzağı olmak üzere toplam 30 buzağı oluşturdu. İstatistiksel analizde, septisemili buzağıların lökosit (WBC), Eritrosit (RBC), hematokrit (Hct) hemoglobin (Hb) ve ortalama korpüsküler hemoglobin konsantrasyonu (MCHC) değerleri kontrol grubunun aynı parametrelerine göre yüksek tespit edilirken (P <0.05), septisemili yenidoğan buzağıların PLT değerleri kontrol grubunun PLT değerlerine göre düşük (P <0.05) saptandı. Septisemik buzağılarda ICAM-1, TNF-α, IL-6, IL-8 ve CRP değerleri kontrol grubunun aynı değerlerine göre anlamlı düzeyde  tespit edildi (P<0.05).

Sonuç olarak, TNF-α, IL-6, IL-8 ve özellikle ICAM-1 konsantrasyonlarının analizlerinin septisemili vakalarda hastalığın şiddetini ve prognozunu değerlendirmede yararlı parametreler olabileceği, bununla birlikte bu parametrelerin  tedavi etkinliğinin takibinde göz önünde bulundurulması gereken parametreler olacağı kanısına varıldı.

Anahtar Kelimeler

• Buzağı
• Septisemi
• Akut Faz Proteini
• Sitokin
• ICAM

Kaynakça

1. Adams JL, Semrad SD, and Czuprynski CJ (1990). Administration of bacterial lipopolysaccharide elicits circulating tumor necrosis factor-alpha in neonatal calves. J Clin Microbiol, 28(5): 998-1001.
2. Akgul, O., Kozat, S., Ozkan, C., Kaya, A., Akgul, Y. (2019). Evaluation of acute phase protein levels and some cytokine levels in pneumonic calves. Med. Weter, 75(3), 152-157.
3. Austgulen R, Arntzen KJ, Haereid PE, Aag S, Dollner H (1997). Infections in neonates delivered at term are associated with increased serum levels of ICAM‐1 and E‐selectin. Acta Paediatrica, 86(3): 274-280.
4. Ay M, Gürbilek M, and Vatansev H (1998). Acute phase proteins. J Gen Intern Med, 8(3): 125-32.
5. Barlow JZ, Huntley GW (2000). Developmentally regulated expression of Thy‐1 in structures of the mouse sensory‐motor system. J Comp Neurol, 421(2), 215-233.
6. Barton MH, Collates C (1999). Tumor necrosis factor and ınterleukin‐6 activity and endotoxin concentration in peritoneal fluid and blood of horses with acute abdominal disease. J Vet Intern Med, 13(5): 457-464.
7. Basoglu A, Sen I, Sevinc M, Simsek A (2004). Serum concentrations of tumor necrosis factor‐a in neonatal calves with presumed septicemia. J Vet Intern Med, 18(2): 238-241.
8. Bayraktar M, Bayraktar N, Bayındır Y, Durmaz R (2005). Brusellozlu hastalarda serum C-reaktif protein, demir ve ferritin düzeylerinin tanı ve izlemdeki değeri. Antibiyotik ve Kemoterapi Derneği, 19(2): 61-3.

9. Bone RC, Balk RA, Cerra FB, Dellinger RP, Fein AM, Knaus WA, Sibbald WJ (1992). Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Chest, 101(6): 1644-1655.
10. Bozukluhan K, Merhan O, Büyük F, Çelebi Ö, Gökçe G (2016). Detection of the levels of some acute phase proteins in cattle with brucellosis. Vet J Ankara Univ, 63: 13-16.
11. Camcıoğlu Y, Aytaç E (2007). Immunopathogenesis of sepsis, Turkish Journal of Intensive Care, 5, 1, 81-85.
12. Caswell JL, Middleton DM, Sorden SD, Gordon JR (1998). Expression of the neutrophil chemoattractant interleukin-8 in the lesions of bovine pneumonic pasteurellosis. Vet Pathol, 35(2), 124-131.
13. Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H (2012). Acute phase proteins in ruminants, J Proteomics, 75, 14, 4207-4231.
14. Cowley HC, Heney D, Gearing AJ, Hemingway I, Webster NR (1994). Increased circulating adhesion molecule concentrations in patients with the systemic inflammatory response syndrome: a prospective cohort study. Crit Care Med, 22(4): 651-657.
15. Çakıroğlu D, Meral Y, Pekmezci D, Onuk EE, Gökalp G (2010). Yeni doğan buzağılarda çeşitli hematolojik ve biyokimyasal parametreler ile kolostral immun globulinler arasındaki ilişkinin belirlenmesi, Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 24, 1, 43-46.
16. Çitil M, Gökçe E (2013). Neonatal septisemi, Turkiye Klinikleri J Vet Sci, 4, 1, 62-70.
17. Dandona P, Nix D, Wilson MF, Aljada A, Love J, Assicot M, Bohuon C L A UD E (1994). Procalcitonin increase after endotoxin injection in normal subjects. J Clin Endocrinol Metab, 79(6), 1605-1608.
18. De Bont E, Martens A, Van Raan J, Samson G, Fetter W, Okken A, De Leij L (1993). Tumor necrosis factor-α, interleukin-1β, and interleukin-6 plasma levels in neonatal sepsis. Pediatric Res, 33(4), 380-383.
19. Deveci U, Ayaz S, Ayaz A, Elevli M (2002). Sepsisli çocuklarda serum interselüler adezyon molekülü-1 düzeyleri. Çocuk Sağlığı ve Hastalıkları Dergisi, 45, 162-168.
20. Dinarello CA (1991). The proinflammatory cytokines interleukin-l and tumor necrosis factor and treatment of the septic shock syndrome. J Infect Dis, 163(6), 1177-1184.
21. Doellner H, Arntzen KJ, Haereid PE, Aag S, Austgulen R (1998). Interleukin-6 concentrations in neonates evaluated for sepsis. J Pediatr, 132(2), 295-299.
22. Endo S, Inada K, Kasai T, Takakuwa T, Yamada Y, Koike S, Yoshida M (1995). Levels of soluble adhesion molecules and cytokines in patients with septic multiple organ failure. J Inflamm 1995-96; 46, 212-219.
23. Ercan N, Tuzcu N, Başbuğ O, Gök K, Işıdan H, ve Oğrak YZ (2014). The evaluation of important biomarkers in healthy cattle. Kafkas Univ Vet Fak Derg, 20(5): 749-755.
24. Fischer S, Bauerfeind R, Czerny CP, Neumann S (2016). Serum interleukin-6 as a prognostic marker in neonatal calf diarrhea. J Dairy Sci, 99(8), 6563-71.
25. Gerros TC, Semrad SD and Proctor RA (1995). Alterations in clinical, hematological and metabolic variables in bovine neonatal endotoxemia. Canadian Journal of Veterinary Research, 59(1): 34.
26. Gökçe Hİ, Bozukluhan K (2009). Important acute phase proteins in farm animals and their use in Veterinary Medicine. Dicle Univ.Journal of Vet Fac, 1, 1, 1-14.
27. Hack CE, De Groot ER, Felt-Bersma RJ, Nuijens JH, Van Schijndel RS, Eerenberg-Belmer AJ and Aarden LA (1989). Increased plasma levels of interleukin-6 in sepsis. Blood, 74(5): 1704-1710.
28. Hirao Y, Kanda T, Aso Y, Mitsuhashi M, Kobayashi I (2000). Interleukin-8—an early marker for bacterial infection. Laboratory Medicine, 31(1), 39-44.
29. Horadagoda NU, Hodgson JC, Moon GM, Wijewardana TG, Eckersall PD (2002). Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B: 2 endotoxin and the role of tumor necrosis factor-α. Res Vet Sci, 72(3), 194-200.
30. Karadal AE (2009). SIRS ve sepsis hastalarında deksmedetomidin ve propofolün immün sistem üzerine etkileri, Çukurova Üniversitesi Tıp Fakültesi Anestezi ve Reaniamsyon Anabilim Dalı, Uzmanlık Tezi, Adana.
31. Kayal S, Jaïs JP, Aguini N, Chaudiere J, Labrousse J (1998). Elevated circulating E-selectin, intercellular adhesion molecule 1, and von Willebrand factor in patients with acute infection. Am J Respir Crit Care Med, 157(3), 776-784.
32. Kozat S, Tuncay I (2018). Prevalance of Rotavirus, Coronavirus, Cryptosprodium spp., Escherichia coli K 99, and Giardia lamblia pathogens in neonatal calves with diarrheic in Siirt Region. Van Vet J, 29 (1), 17-22.
33. Kozat S (2018). Hypothermia in newborn calves. Ist Vet Sci, 2(1), 30-37.
34. Kozat S, Ozkan C (2018). Intercellular adhesion molecule-1 (ICAM-1) and C-Reactive Protein (CRP) levels in calves naturally infected with Mycoplasma bovis. Int J Vet and Animal Res, 1(2), 34-37.
35. Lee WC, Hsiao HC, Wu YL, Lin JH, Lee YP, Fung HP, Chu RM (2003). Serum C-reactive protein in dairy herds. Can J Vet Res, 67(2), 102.
36. Lofstedt J, Dohoo IR, Duizer G (1999). Model to predict septicemia in diarrheic calves. J Vet Intern Med, 13(2), 81-88.
37. MacKay RJ, Merritt AM, Zertuche JM, Whittington M, Skelley LA (1991). Tumor necrosis factor activity in the circulation of horses given endotoxin. Am J Vet Res, 52(4), 533-538.
38. Mackay CR, Imhof BA (1993). Cell adhesion in the immune system. Immunol Today, 14(3), 99-102.
39. Martin H, Olander B, Norman M (2001). Reactive hyperemia and interleukin 6, interleukin 8, and tumor necrosis factor-α in the diagnosis of early-onset neonatal sepsis. Pediatrics, 108(4), 61-6.
40. Mehr SS, Doyle LW, Rice GE, Vervaart P, Henschke P (2001). Interleukin-6 and interleukin-8 in newborn bacterial infection. Am J Perinatol, 18(06), 313-324.
41. Michaels FH, Banks KL (1988). Contribution of various host factors to resistance to experimentally induced bacterial endotoxemia in calves. Am J Vet Res, 49(4), 557-562.
42. Molina EC (2005). Serum interferon-gamma and interleukins-6 and-8 during infection with Fasciola gigantica in cattle and buffaloes. Am J Vet Res, 6(2), 135-139.
43. Morris DD, Crowe N, Moore JN (1990). Correlation of clinical and laboratory data with serum tumor necrosis factor activity in horses with experimentally induced endotoxemia. Am J Vet Res, 51(12): 1935-1940.
44. Morris DD, Moore JN, Crowe N, Moldawer LL (1992). Effect of experimentally induced endotoxemia on serum interleukin-6 activity in horses. Am J Vet Res, 53(5), 753-756.
45. Murtaugh MP, Baarsch MJ, Zhou Y, Scamurra RW, Lin G (1996). Inflammatory cytokines in animal health and disease. Vet Immunol Immunopathol, 54(1-4), 45-55.
46. Nakajima Y, Mikami O, Yoshioka M, Motoi Y, Ito T, Ishikawa Y, Yasukawa K (1997). Elevated levels of tumor necrosis factor-α,(TNF-α) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis. Res Vet Sci, 62(3), 297-298.
47. Natanson C, Eichenholz PW, Danner RL, Eichacker PQ, Hoffman WD, Kuo GC and Parrillo JE (1989). Endotoxin and tumor necrosis factor challenges in dogs simulate the cardiovascular profile of human septic shock. J Exp Med, 169(3), 823-832.
48. Okusawa S, Gelfand JA, Ikejima T, Connolly RJ, Dinarello C (1988). Interleukin 1 induces a shock-like state in rabbits. Synergism with tumor necrosis factor and the effect of cyclooxygenase inhibition. J Clin Invest, 81(4), 1162.
49. Petersen HH, Nielsen JP, Heegaard PMH (2004). Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35(2): 163-187.
50. Pohlman TH, Stanness KA, Beatty PG, Ochs HD, Harlan JM (1986). An endothelial cell surface factor (s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism. J Immunol, 136(12): 4548-4553.
51. Rothstein JL, Schreiber H (1988). Synergy between tumor necrosis factor and bacterial products causes hemorrhagic necrosis and lethal shock in normal mice. Proc Natl Acad Sci U S A, 85(2), 607-11.
52. Sessler CN, Windsor AC, Schwartz M, Watson L, Fisher BJ, Sugerman HJ, Fowler AA (1995). Circulating ICAM-1 is increased in septic shock. Am J Respir Crit Care Med, 151(5): 1420-1427.
53. Steel DM , Whitehead AS (1994). The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein, Immunol Today, 15, 2, 81-88.
54. Terekeci MH, Şahan B, Top C (2008). Hücre adezyon molekülleri. Nobel Medıcus, 4(1), 4-10.
55. Waage S, Brandtzaeg P, Halstensen A, Kierulf P, Espevik T (1989). The complex pattern of cytokines in serum from patients with meningococcal septic shock. Association between interleukin 6, interleukin 1, and fatal outcome. J Exper Med, 169(1): 333-338.
56. Wakabayashi G, Gelfand JA, Jung WK, Connolly RJ, Burke JF, Dinarello CA (1991). Staphylococcus epidermidis induces complement activation, tumor necrosis factor and interleukin-1, a shock-like state and tissue injury in rabbits without endotoxemia. Comparison to Escherichia coli. J Clin Invest, 87(6): 1925.
57. Weinberg JR, Wright DJM, Guz A (1988). Interleukin-1 and tumour necrosis factor cause hypotension in the conscious rabbit. Clin Sci, 75(3): 251-255.
58. Whalen MJ, Doughty LA, Carlos TM, Wisniewski SR, Kochanek PM adn Carcillo JA (2000). Intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 are increased in the plasma of children with sepsis-induced multiple organ failure. Crit Care Med, 28(7): 2600-2607.