Rat Böbrek Dokusunda Kurşunun Neden Olduğu Oksidatif Strese Karşı Kitosanın Koruyucu etkisi

Yıl 2019, , 187 - 191, 22.11.2019

Ugur Özdek , Hasan Toz Ahmet Ufuk Kömüroğlu , Leyla Mis , Zübeyir Huyut , Yeter Değer

https://doi.org/10.36483/vanvetj.629310

Cited By: 5

Öz

Kitosan antioksidan ve
şelatör özelliğe sahip doğal bir polimerdir. Bu çalışmada kurşun toksikosyonu
oluşturulmuş ratların böbrek dokusu kurşun (Pb), molandialdehit (MDA),
8-hidroksi deoksiguanozin (8-OHdG), glutatyon (GSH), seroloplazmin
konsantrasyonu ve katalaz aktivitesi üzerine kitosanın etkisi araştırıldı. Her
grupta sekiz adet olacak şekilde 28 adet erkek Wistar albino rat, kontrol (C),
kurşun grubu (Pb grubu), kurşun+kitosan (Pb+CS grubu) ve kitosan (CS grubu)
olmak üzere dört gruba ayrıldı. Kurşun grubuna 5 gün, 50 mg/kg kurşun asetat
intraperitonel (ip) ve kitosan gruplarına (CS+Pb ve CS grupları) 28 gün boyunca
200 mg/kg kitosan gavaj yoluyla uygulandı. Çalışma sonunda, kurşun, MDA,
8-OHdG, seruloplazmin, GSH konsantrasyonu ve katalaz aktivitesi böbrek
dokusunda ölçüldü. Kontrol grubu ile karşılaştırıldığında, Pb uygulanan
gruplarda böbrek dokusunda Pb, MDA, 8-OHdG ve seruloplazmin seviyesi arttı, GSH
seviyesi ile katalaz aktivitesi ise azaldı (p<0.05). Kurşun ile birlikte
kitosan verilmesi böbrek dokusunda Pb, MDA ve seruloplazmin seviyelerini
azalttı, CAT aktivitesini arttırdı (p<0.05). GSH ve 8-OHdG seviyelerinde önemli
değişiklik olmadı (p>0.05). Elde edilen sonuçlar, kitosanın, kurşun
uygulaması oluşan oksidatif streseten böbreği koruduğunu göstermektedir.Her
grupta sekiz adet olacak şekilde 28 adet male Wistar albino rat control (C),
lead group (Pb group), lead+ chitosan (Pb+CS group) ve chitosan (CS group)
olmak üzere dört gruba ayrıldı. Lead group were administered 50 mg/kg lead
acetate intraperitoneal (ip) for 5 days and chitosan groups (CS+Pb and CS
groups) were adminestered 200 mg/kg chitosan for 28 days via gavage. At the end
the study, lead, MDA, 8-OHdG, seruloplazmin, GSH konsantrasyonu ve katalaz
aktivitesi böbrek dokusunda ölçüldü.

Kontrol
grubu ile karşılaştırıldığında, Pb uygulanan gruplarda böbrek dokusunda Pb,
MDA, 8-OHdG ve seruloplazmin seviyesi arttı, GSH seviyesi ile katalaz
aktivitesi ise azaldı (p<0.05). Kurşun ile birlikte kitosan verilmesi böbrek
dokusunda Pb, MDA ve seruloplazmin seviyelerini azalttı, CAT aktivitesini
arttırdı (p<0.05). GSH ve 8-OHdG seviyelerinde önemli değişiklik olmadı
(p>0.05).

Elde
edilen sonuçlar, kitosanın, kurşun uygulaması oluşan oksidatif streseten
böbreği koruduğunu göstermektedir.

Anahtar Kelimeler

Kitosan, Kurşun, Böbrek, Oksidatif stres

Destekleyen Kurum

VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ BAŞKANLIĞI

Proje Numarası

2015-SBE-YL049

Teşekkür

Desteklerineden dolayı VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ BAŞKANLIĞI'na teşekkür ederiz.

Protective Effect of Chitosan Against Lead-Induced Oxidative Stress in Rat Kidney

Öz

                        Chitosan is a natural polymer with antioxidant and chelating
properties. This study investigated the effects of chitosan on lead (Pb),
malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), glutathione (GSH),
ceruloplasmin concentrations and catalase (CAT) activity in the kidney tissue
of the rats exposed to lead. 28 male Wistar albino rats were divided into four
groups of eight each: control (C), lead group (Pb group), lead+ chitosan (Pb+CS
group), and chitosan (CS group). The lead group was administered 50 mg/kg lead
acetate intraperitoneally (ip) for 5 days, and the chitosan groups (CS+Pb and
CS groups) were administered 200 mg/kg chitosan for 28 days via gavage. At the
end of the study, Pb, MDA, 8-OHdG, ceruloplasmin, GSH concentrations and
catalase activity were measured in the kidney tissue. In the Pb-treated groups
when compared with the control group, Pb, MDA, 8-OHdG, ceruloplasmin
concentrations were significantly increased, and GSH concentration and catalase
activity were significantly decreased (p<0.05). Coadministration of chitosan
with lead significantly decreased Pb, MDA, and ceruloplasmin levels and
significantly increased CAT activity in the kidney tissue (p<0.05). There
were no significant changes in GSH and 8-OHDG levels (p>0.05). The obtained
results show that chitosan protects the kidney against lead-induced oxidative
stress.

Anahtar Kelimeler

Chitosan, Lead, Kidney, Oxidative stress

Proje Numarası

2015-SBE-YL049

Kaynakça

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