Inovatif Gastroentero-Dermatoloji Kapsamında Muhtelif Yöntem Geliştirme I: Lactobacillus plantarum ve Lactobacillus paracasei ile Probiyotik Eneması Atopik Dermatitli Köpeklerde Anti-Pruritik Etkinlik Sağlar Mı?

Öz

İnsanlarda önemli bir dermatolojik problem olan atopik dermatit (Ad) köpekler içinde benzer durum teşkil etmektedir. Ad’ li hastalarda sızıntılı bağırsak ilişkili intestinal permeabilite artışı ve bağırsak mikrobiyatasının bozulmasının hastalıkta rol oynadığı bilinmesine rağmen hala bu konuda gerek tanı gerekse sağaltım anlamında bilinmeyenler mevcuttur. Bu çalışmada Ad ile bağırsak mikrobiyatası arasındaki ilişki baz alınarak; 1) en önde gelen semptom olan kaşıntının sağaltımında Lactobacillus plantarum ve Lactbacillus paracasei ile probiyotik enemasının kullanılıp kullanılamayacağının belirlenmesi, 2) rol model sağaltım için umut vadeden yeni, güncel probiyoterapi seçeneklerinden birisinin oral uygulamadaki emilimdeki sorunların aksine rektal enema, diğer adıyla proktolizis, yolu ile uygulanmasının değerlendirilmesi amaçlanmıştır. Çalışmamıza baskın klinik bulgu olarak kaşıntısı bulunan toplamda 12 farklı yaş, ırk ve her iki cinsiyetteki Ad’li köpek dahil edilmiştir. Daha önce sağaltım geçmişi olmayan, Favrot kriterleri ve atopi ile uyumlu klinik bulgulara eşlik eden alerjen-spesifik IgE düzeyinde artış şekillenmiş olgular (sağaltım öncesi), CADESI-04 skorları ve klinik bulgular eşliğinde belirlendikten sonra rektal enema yolu ile nutrasötiklerle desteklenmiş Lactobacillus plantarum ve Lactbacillus paracasei ile probiyotik eneması foley kateteri ya da rektal kateter vasıtası ile rektumdan 10-15 cm ileriye uygulandı. Ad’ li ve rektal enema ile probiyoterapi uygulanan 12 olguda kaşıntı 0 ila 10. günler arasında belirgin şekilde kesildi. Sonuç olarak antipruritik laktik asit bakterileri içerisinde değerlendirilebilecek olan L. plantarum ve L. paracasei suşlarının, bu araştırmada önerilen ve uygulanan dozda/sürede, atopik dermatitli köpeklerde hem klinik iyileşme hem de kaşıntının giderilmesi amacıyla kullanılabileceği görülmüştür.

Anahtar Kelimeler

• atopik dermatit
• kaşıntı
• köpek
• probiyoterapi

Development of Various Methods in Innovative Gastroentero-Dermatology I: Does Probiotic Enema with Lactobacillus plantarum and Lactobacillus paracasei Provide Anti-Pruritic Efficacy in Dogs with Atopic Dermatitis?

Öz

Atopic dermatitis (Ad), an important dermatological problem for humans, similar to dogs. Although it is known that leaky gut-related increase in intestinal permeability and deterioration of the intestinal microbiota play a role in the disease, there are still unknowns regarding in term of both diagnosis and treatment. In this study, based on the relationship between atopic dermatitis and intestinal microbiota; 1) determination of whether Lactobacillus plantarum and Lactbacillus paracasei and probiotic enema can be used in the treatment of itching, which is the leading symptom, 2) aimed to evaluate the administration of one of the new, up-to-date probiotherapy options that are promising for role model treatment by rectal enema, also known as proctolysis, in contrast to problems of absorption in oral administration. In our study, a total of 12 dogs with Ad as different ages, breeds, and both sexes were included. Cases which had no previous treatment history, showed clinical findings compatible with Favrot criteria and atopy accompanying with increasing allergen-specific IgE level, were determined in the light of allergen-specific IgE levels (before treatment), CADESI-04 scores and clinical findings, and then rectal enema with nutraceuticals. Probiotic enema with Lactobacillus plantarum and Lactbacillus paracasei was applied 10-15 cm forward from the rectum via foley catheter or rectal catheter. Pruritus of 12 dogs with Ad applied probiotherapy by rectal enema were ceased significantly between 0 and 10 days. In conclusion, it has been observed that L. plantarum and L. paracasei strains, which can be evaluated in antipruritic lactic acid bacteria, can be used for both clinical improvement and relief of itching in dogs with atopic dermatitis at the recommended and applied dose/time in this study.

Anahtar Kelimeler

• atopic dermatitis
• dog
• probiotherapy
• pruritis

Kaynakça

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