In Vitro Ortamda Kalsiyum Klorürün Osteoblast Benzeri Hücreler Üzerine Etkisi

Öz

Bu çalışmada in vitro ortamda kalsiyum klorürün osteoblast benzeri hücrelerin proliferasyonu üzerine cinsiyet bağımlı olarak etkisi olup olmadğının belirlenmesi ve osteoblast benzeri hücrelerin proliferasyonlarını etkileyen en uygun konsantrasyonun belirlenmesi amaçlanmıştır. Her iki cinsiyetten 90- 95 günlük ratların kemik ilikleri çalışmanın biyolojik materyalini oluşturmuştur. Altı farklı kalsiyum klorür konsantrasyonu test edildi ve 24 ve 48 saat sonra osteoblast benzeri hücrelerin sayısal değerleri tespit edildi. 24 saat sonunda erkekler ve dişiler arasındaki ortalama değerlerin test sonuçları, 0.25 mM ve 1.8 mM kalsiyum klorür konsantrasyonları istatistiki olarak anlamlı derecede farklılık göstermiştir(P<0.05). 48 saat sonraki sonuçlar, çoğu CaCl2 konsantrasyonunda önemli farklılıklar olmadığını gösterdi. Erkek osteoblast benzeri hücrelerin, dişi osteoblast benzeri hücrelere kıyasla farklı kalsiyum klorür konsantrasyonları için daha yüksek bir afinite gösterdiği belirlendi. 0.25 mM'lik kalsiyum klorür konsantrasyonu osteoblast benzeri hücrenin proliferasyonunu en olumlu şekilde etkileyen konsantrasyon olarak belirlenmiştir. Bu konsantrasyonda proliferasyon kontrol değerine oranla %26.6 daha fazla olduğu belirlenmiştir.

Anahtar Kelimeler

• Kemik iliği
• kalsiyum klorür
• hücre proliferasyonu
• osteoblast

Influence of Calcium Chloride on Osteoblast Like Cells of Both Sexes in Rats in In Vitro Conditions

Öz

The aim of the study was to determine whether calcium chloride affects the proliferation of osteoblast like cells in a sex-dependent manner, as well as to determine the most effective concentration on proliferation of osteoblast like cells, in in vitro conditions. Bone marrow was used as biological material from young adult rats, both sexes, aged 90-95 days. Six different concentrations of calcium chloride were tested, determining the numerical representation of osteoblast like cells after 24 and 48 hours. Test results of mean values between males and females after 24 hours, indicate significant differences with a probability of P<0.05 at calcium chloride concentrations of: 0.25 mM and 1.8 mM. Results after 48 hours showed that there were no significant differences at most CaCl2 concentrations. It was found that male osteoblast like cells show a higher affinity for different calcium chloride concentrations when compared to the female osteoblast like cells. The calcium chloride concentration of 0.25 mM affected the proliferation of osteoblast like cells most favorably, which is 26.6% higher than the control values.

Anahtar Kelimeler

• osteoblast
• bone marrow
• Calcium chloride
• Cell proliferation

Kaynakça

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